Photostability and Skin Penetration of Different E‐Resveratrol‐Loaded Supramolecular Structures

Abstract

It is desirable and challenging to prevent E-resveratrol (E-RSV) from photoisomerizing to its Z-configuration to preserve its biological and pharmacological activities. The aim of this research was to evaluate the photostability of E-RSV-loaded supramolecular structures and the skin penetration profile of chemically and physically stable nanoestructured formulations. Different supramolecular structures were developed to act as carriers for E-RSV, that is, liposomes, polymeric lipid-core nanocapsules and nanospheres and solid lipid nanoparticles. The degrees of photostability of these formulations were compared with that of an ethanolic solution of E-RSV. The skin penetration profiles of the stable formulations were obtained using vertical diffusion cells (protected from light and under UVA radiation) with porcine skin as the membrane, followed by tape stripping and separation of the viable epidermis and dermis in a heated water bath. Photoisomerization was significantly delayed by the association of resveratrol with the nanocarriers independently of the supramolecular structure. Liposomes were the particles capable of maintaining E-RSV concentration for the longest time. On the other hand, E-RSV-loaded liposomes reduced in size showing low physical stability under UVA radiation. In the dark, the skin penetration profiles were very similar, but under UVA radiation the E-RSV-loaded nanocarriers showed increasing amounts in the total epidermis.

Testing of resveratrol microemulsion photostability and protective effect against UV induced oxidative stress

“…UV radiation and temperature induce rapid transformation of trans-resveratrol into the cis-isomeric form, resulting in decreased biological activity (4). The trans-isoform of resveratrol should be protected until its delivery to the site of action in skin tissues.…”

Current application of phytocompound-based nanocosmeceuticals for beauty and skin therapy

“…252 Further modification of bioactive compounds along with the nanodelivery technologies can enhance skin bioavailability to increase protection. 145 Similarly, quercetin encapsulated into a solid lipid nanoparticle system showed increased skin retention of quercetin with greater antioxidant activity. 138,253,254 Genistein-loaded nanoemulsions were incorporated into hydrogels in the size range of 250 nm and enhanced delivery of the bioactive compound to the skin for a beauty-based therapy.…”

“…144 Further E-resveratrol greatly protects skin through the lipid-based nanoparticle system, under UV radiation. 145 In certain studies, some bioactive compounds, such as squalene, promote drug absorption to the hair follicles, for example, a squalene-enriched nanostructured lipid carrier with a particle size of 208-265 nm is used to treat hair follicles of individuals suffering from alopecia areata. 146 From these recent research studies, we confirm that nanostructured lipid carrier systems will open a gateway for phytobioactive compounds to enhance beauty and personal care.…”

“…Similarly, an individual phytocompound of resveratrol was constructed using lipid-derived nanocapsules at 207 nm that enhanced skin protection with higher bioavailability of those bioactive compounds under UV radiation. 145 Chitosan hydrogels combine nanocapsules with the active compounds of capsaicinoids and enhance skin protection by the control release of those compounds. [171][172][173] The same research group studied the effects of capsaicinoids 172 These studies confirm that phyto-derived bioactive compounds can be used for the construction of nanocapsules to apply in cosmeceutical sectors and beauty therapies.…”

V Congress of the Portuguese Society of Cosmetological Sciences - Proceedings

“…RES occurs as cis-(Z) and trans-(E) isomeric forms in nature, of which trans RES is the more photo and thermally stable, more common and the more biologically active form [4,5]. Fast trans-cis isomerization occurs after heating or ultraviolet irradiation exposure which limits RES efficacy [6,7]. This work aimed to clarify the interest of a novel synthetic resveratrol derivative (Resveratrol Glucoside Sulfate-RGS) for skin care, by conducting a preliminary study regarding its stability, safety and efficacy.…”