Photosynthetic conversion of carbon dioxide to ethylene by the recombinant cyanobacterium, Synechococcus sp. PCC 7942, which harbors a gene for the ethylene-forming enzyme of Pseudomonas syringae

Sakai, Miho; Ogawa, Takahira; Matsuoka, Masayoshi; Fukuda, Hideo

doi:10.1016/s0922-338x(97)82004-1

Cited by 70 publications

(59 citation statements)

References 23 publications

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“…Similarly, the Synechococcus PCC7942 strain harboring the Pseudomonas syringae gene ( efe ) encoding the ethylene-forming enzyme (Fukuda et al, 1992; Sakai et al, 1997), managed to introduce short nucleotide insertions in efe to stop ethylene production and recover a healthy growth (Takahama et al, 2003). Another recombinant Synechococcus PCC7942 strain could introduce a missense mutation in the E. coli atoD gene (acetoacetyl-CoA transferase) to decrease isopropanol production (Kusakabe et al, 2013).…”

Section: Resultsmentioning

confidence: 99%

Comparative Genomics of DNA Recombination and Repair in Cyanobacteria: Biotechnological Implications

2016

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Cyanobacteria are fascinating photosynthetic prokaryotes that are regarded as the ancestors of the plant chloroplast; the purveyors of oxygen and biomass for the food chain; and promising cell factories for an environmentally friendly production of chemicals. In colonizing most waters and soils of our planet, cyanobacteria are inevitably challenged by environmental stresses that generate DNA damages. Furthermore, many strains engineered for biotechnological purposes can use DNA recombination to stop synthesizing the biotechnological product. Hence, it is important to study DNA recombination and repair in cyanobacteria for both basic and applied research. This review reports what is known in a few widely studied model cyanobacteria and what can be inferred by mining the sequenced genomes of morphologically and physiologically diverse strains. We show that cyanobacteria possess many E. coli-like DNA recombination and repair genes, and possibly other genes not yet identified. E. coli-homolog genes are unevenly distributed in cyanobacteria, in agreement with their wide genome diversity. Many genes are extremely well conserved in cyanobacteria (mutMS, radA, recA, recFO, recG, recN, ruvABC, ssb, and uvrABCD), even in small genomes, suggesting that they encode the core DNA repair process. In addition to these core genes, the marine Prochlorococcus and Synechococcus strains harbor recBCD (DNA recombination), umuCD (mutational DNA replication), as well as the key SOS genes lexA (regulation of the SOS system) and sulA (postponing of cell division until completion of DNA reparation). Hence, these strains could possess an E. coli-type SOS system. In contrast, several cyanobacteria endowed with larger genomes lack typical SOS genes. For examples, the two studied Gloeobacter strains lack alkB, lexA, and sulA; and Synechococcus PCC7942 has neither lexA nor recCD. Furthermore, the Synechocystis PCC6803 lexA product does not regulate DNA repair genes. Collectively, these findings indicate that not all cyanobacteria have an E. coli-type SOS system. Also interestingly, several cyanobacteria possess multiple copies of E. coli-like DNA repair genes, such as Acaryochloris marina MBIC11017 (2 alkB, 3 ogt, 7 recA, 3 recD, 2 ssb, 3 umuC, 4 umuD, and 8 xerC), Cyanothece ATCC51142 (2 lexA and 4 ruvC), and Nostoc PCC7120 (2 ssb and 3 xerC).

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Section: Resultsmentioning

confidence: 99%

Comparative Genomics of DNA Recombination and Repair in Cyanobacteria: Biotechnological Implications

2016

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“…21 Our findings were consistent with those reported by Sakai and coworkers, who also emphasized the importance of promoters. 19 According to the results of promoter screening, PcpcB was used for ethylene production in the following study.…”

Section: Promoter Screening For Higher Ethylene Productivitymentioning

confidence: 99%

Enhancing photosynthetic production of ethylene in genetically engineered Synechocystis sp. PCC 6803

Zhu

Xie

et al. 2015

Green Chem.

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Ethylene is widely used in the petrochemical industry and has traditionally been produced via the steam cracking of petroleum-based feedstock. The exploration of sustainable and carbon-neutral methods of producing ethylene from the renewable feedstock seems promising. The direct photosynthetic production of ethylene after the recycling of carbon dioxide shows great potential. In this study, continuous and stable ethylene production was achieved in Synechocystis sp. PCC 6803 by introducing a codonoptimized ethylene-forming enzyme (EFE) from Pseudomonas syringae pv. sesami and using 2-oxoglutarate (2-OG) as the substrate. Based on diverse promoter screening, PcpcB was proved to be a highly efficient promoter for ethylene production in cyanobacteria. The genes encoding 2-OG decarboxylase (OGDC) and succinic semialdehyde dehydrogenase (SSADH) in the tricarboxylic acid (TCA) cycle in Synechocystis sp. PCC 6803 were identified, and the TCA cycle was genetically modified by blocking these two enzymes with the simultaneous overexpression of EFE. Meanwhile, a gene encoding 2-OG permease (KgtP) from E. coli was introduced into the phaAB loci to increase the 2-OG supply. A peak volumetric production rate of 9.7 mL L −1 h −1 for ethylene was eventually achieved in the Synechocystis recombinant (XX110), with the genetic modification of the TCA cycle and heterologous expression of 2-oxoglutarate permease by the modified semi-continuous cultivation.

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“…Further, photosynthetic conversion of carbon dioxide to ethylene by recombinant cyanobacterium Synechococcus sp. PCC 7942 was reported by Sakai et al [67].…”

Section: Potentail Productsmentioning

confidence: 87%

“Green” Chemicals from Renewable Agricultural Biomass - A Mini Review

Xu¹,

Hanna²,

Isom³

2008

TOASJ

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Abstract:Recently, utilization of renewable resources to replace petroleum as a primary feedstock for liquid fuels, chemicals and materials has become a topic of interest around the world. It is intriguing due to rising oil prices, the negative effects of petroleum on the environment and the advantages of renewable resources, such as their abundance and sustainability. Herein, the possibilities for biobased chemicals prepared from renewable resources are reviewed. The most popular feedstocks for commodity and specialty chemicals are carbohydrates as they account for approximately 95% of the biomass produced annually. The conversion routes, including chemical and biological routes, direct extraction, and selected technical advancements are discussed. Examples of select biochemcials, their conversion pathways from biomass, and their derivatives and potential applications are indentified.

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Photosynthetic conversion of carbon dioxide to ethylene by the recombinant cyanobacterium, Synechococcus sp. PCC 7942, which harbors a gene for the ethylene-forming enzyme of Pseudomonas syringae

Cited by 70 publications

References 23 publications

Comparative Genomics of DNA Recombination and Repair in Cyanobacteria: Biotechnological Implications

Comparative Genomics of DNA Recombination and Repair in Cyanobacteria: Biotechnological Implications

Enhancing photosynthetic production of ethylene in genetically engineered Synechocystis sp. PCC 6803

“Green” Chemicals from Renewable Agricultural Biomass - A Mini Review

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