Photosynthetic response and transcriptomic profiling provide insights  into the alkali tolerance of clone halophyte Leymus chinensis

Wang, H.; Xiang, Yun; Li, L.H.; Bhanbhro, Nadeem; Yang, Chen; Zhang, Z.

doi:10.32615/ps.2020.027

Cited by 7 publications

(7 citation statements)

References 48 publications

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“…Massive accumulation of surplus electrons in the electron transport chain due to a decrease of electron transfer rates causes electron leakage and then accelerates the degree of breakage of PSII reaction center under salt stress ( Zhang et al, 2017 ; Hou et al, 2018 ). In addition, Wang et al (2020) have clarified that compared with salt stress, alkali stress decreased the photosynthetic electron transport rate and stomatal conductance, and also reduced the aerenchyma amount, and then influenced the CO 2 influx into mesophyll cells of Leymus chinensis . Drought stress directly makes the stomata closed, resulting in a reduction of CO 2 absorption and transportation of non-structural carbon (NSC), which is an essential element of the photosynthetic system ( Steppe et al, 2015 ; Hajiboland et al, 2017 ).…”

Section: Introductionmentioning

confidence: 98%

Uniform Water Potential Induced by Salt, Alkali, and Drought Stresses Has Different Impacts on the Seedling of Hordeum jubatum: From Growth, Photosynthesis, and Chlorophyll Fluorescence

et al. 2021

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Hordeum jubatum is a halophyte ornamental plant wildly distributed in the Northeast of China, where the low water potential induced by various abiotic stresses is a major factor limiting plant growth and development. However, little is known about the comparative effects of salt, alkali, and drought stresses at uniform water potential on the plants. In the present study, the growth, gas exchange parameters, photosynthetic pigments, and chlorophyll fluorescence in the seedlings of H. jubatum under three low water potentials were measured. The results showed that the growth and photosynthetic parameters under these stresses were all decreased except for carotenoid (Car) with the increasing of stress concentration, and alkali stress caused the most damaging effects on the seedlings. The decreased net photosynthetic rate (Pn), stomatal conductance (Gs), and intercellular CO2 concentrations (Ci) values under salt stress were mainly attributed to stomatal factors, while non-stomatal factors were dominate under drought and alkali stresses. The reduced chlorophyll and slightly increased Car contents occurred under these stresses, and most significant changed under alkali stress. In addition, the maximum photochemical efficiency (Fv/Fm), actual photochemical efficiency (ΦPSII), and photochemical quenching coefficient (qP) under the stresses were all decreased, indicating that salt, alkali, and drought stresses all increased susceptibility of PSII to photoinhibition, reduced the photosynthetic activity by the declined absorption of light for photochemistry, and increased PSII active reaction centers. Moreover, the non-photochemical quenching coefficient (NPQ) of alkali stress was different from salt and drought stresses, showing that the high pH of alkali stress caused more damaging effects on the photoprotection mechanism depending on the xanthophyll cycle. The above results suggest that the H. jubatum has stronger tolerance of salt than drought and alkali stresses, and the negative effects of alkali stress on the growth and photosynthetic performance of this species was most serious.

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Section: Introductionmentioning

confidence: 98%

Uniform Water Potential Induced by Salt, Alkali, and Drought Stresses Has Different Impacts on the Seedling of Hordeum jubatum: From Growth, Photosynthesis, and Chlorophyll Fluorescence

et al. 2021

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“…The application of organic acids immediately lowers the soil pH ( Rukshana et al., 2014 ), and plants, such as grape hybrid rootstocks, seabuckthorn, Chloris virgata , wheat and grapevine, can mitigate the damage caused by alkali stress through the secretion of organic acids ( Chen et al., 2009 ; Yang et al., 2010 ; Guo et al., 2018 ; Xiang et al., 2019 ; Li et al., 2020 ). Our previous study revealed that alkali stress strongly inhibits photosynthesis and growth of L. chinensis , and it disturbed the ion balance ( Wang et al., 2020 ). Here, our results indicated that L. chinensis extensively secreted organic acids, phenolic acids, free fatty acids and other substances containing a -COOH or phosphate group after expose to alkali stress.…”

Section: Discussionmentioning

confidence: 99%

Carboxylic acid accumulation and secretion contribute to the alkali-stress tolerance of halophyte Leymus chinensis

Wang,

Zhao,

Sun

et al. 2024

Front. Plant Sci.

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Leymus chinensis is a dominant halophytic grass in alkalized grasslands of Northeast China. To explore the alkali-tolerance mechanism of L. chinensis, we applied a widely targeted metabolomic approach to analyze metabolic responses of its root exudates, root tissues and leaves under alkali-stress conditions. L. chinensis extensively secreted organic acids, phenolic acids, free fatty acids and other substances having -COOH or phosphate groups when grown under alkali-stress conditions. The buffering capacity of these secreted substances promoted pH regulation in the rhizosphere during responses to alkali stress. L. chinensis leaves exhibited enhanced accumulations of free fatty acids, lipids, amino acids, organic acids, phenolic acids and alkaloids, which play important roles in maintaining cell membrane stability, regulating osmotic pressure and providing substrates for the alkali-stress responses of roots. The accumulations of numerous flavonoids, saccharides and alcohols were extensively enhanced in the roots of L. chinensis, but rarely enhanced in the leaves, under alkali-stress conditions. Enhanced accumulations of flavonoids, saccharides and alcohols increased the removal of reactive oxygen species and alleviated oxygen damage caused by alkali stress. In this study, we revealed the metabolic response mechanisms of L. chinensis under alkali-stress conditions, emphasizing important roles for the accumulation and secretion of organic acids, amino acids, fatty acids and other substances in alkali tolerance.

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“…Plants can respond to salt and alkali stress by accumulating small-molecule osmotic solutes in order to reduce stress damage [6]. Previous research has revealed that when plants are subjected to salt and alkali stress, the concentrations of soluble sugars [14,15,21], organic acids [12,13,15,23,24], amino acids [6,25], and betaine [21] increase, which are the main solutes to resist stress. However, the solutes accumulated by plants in response to salt and alkali stress change depending on different plant species and stress concentrations.…”

Section: Changes In Organic Solutes Under Salt and Alkali Stressmentioning

confidence: 99%

“…As a result, it is one of the most suitable grass species for salinealkali land improvement [1,12]. Currently, researchers are investigating the response of L. chinensis to salt and alkali stress using transcriptome and proteome approaches [13,14], while the physiological response of L. chinensis to salt and alkali stress is currently being investigated only through multiple physiological experiments to study the accumulation of specific metabolites under salt and alkali stress. L. chinensis enhances salt tolerance predominantly by accumulating proline, betaine, and soluble sugar, and alkali tolerance primarily by accumulating organic acids (citric acid, malic acid, succinic acid, acetic acid, and oxalic acid) [12,15].…”

Section: Introductionmentioning

confidence: 99%

Differences in Organic Solute and Metabolites of Leymus chinensis in Response to Different Intensities of Salt and Alkali Stress

Yan

Shi

et al. 2023

Plants

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To explore differences in the physiological metabolic response mechanisms of grassland perennial plants to different intensities of salt–alkali stress, we employed GC-MS to identify the metabolome of perennial rhizome-saline-tolerant Leymus chinensis (L. chinensis). L. chinensis reduced stress damage by accumulating osmotic solutes during salt–alkali stress, although the types of accumulated solutes varied with stress and concentration gradients. Soluble sugars increased only under mild salt–alkali stress. Under salt and mild alkali stress, amino acids increased. Under severe salt–alkali stress, organic acids increased. Betaine increased as a typical osmolute under salt–alkali stress. Metabolic analysis identified 20 metabolites, including 4 amino acids, 6 sugars, and 10 organic acids. The majority of them increased in response to stress. Under mild salt stress, the metabolites included glycine and proline. Under mild alkali stress, they primarily consisted of sugars such as isomaltose and lactulose, whereas under severe salt–alkali stress, they primarily consisted of organic acids such as citric acid and isocitric acid. Pathway analysis showed that six pathways were affected. Glycine, serine, and threonine metabolism was affected under mild salt stress. Alanine, aspartate, and glutamate metabolism and butanota metabolism were affected under mild alkali stress, while energy metabolism pathways, such as the TCA cycle and glyoxylate and dicarboxylate metabolism, were affected under severe salt–alkali stress. The results indicate the importance of betaine in stress resistance and the significance of organic acid in severe salt stress, and they also demonstrate that energy supply was one of the key mechanisms in response to severe salt–alkali stress.

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Photosynthetic response and transcriptomic profiling provide insights into the alkali tolerance of clone halophyte Leymus chinensis

Cited by 7 publications

References 48 publications

Uniform Water Potential Induced by Salt, Alkali, and Drought Stresses Has Different Impacts on the Seedling of Hordeum jubatum: From Growth, Photosynthesis, and Chlorophyll Fluorescence

Uniform Water Potential Induced by Salt, Alkali, and Drought Stresses Has Different Impacts on the Seedling of Hordeum jubatum: From Growth, Photosynthesis, and Chlorophyll Fluorescence

Carboxylic acid accumulation and secretion contribute to the alkali-stress tolerance of halophyte Leymus chinensis

Differences in Organic Solute and Metabolites of Leymus chinensis in Response to Different Intensities of Salt and Alkali Stress

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