Photosystem I Protein Films at Electrode Surfaces for Solar Energy Conversion

LeBlanc, Gabriel; Gizzie, Evan A.; Yang, Siyuan; Cliffel, David E.; Jennings, G. Kane

doi:10.1021/la500129q

Cited by 66 publications

(73 citation statements)

References 64 publications

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“…11,12 In-depth summaries of progress in this field of RC-based biophotolectrodes, including parallel work using photosystem I (PSI) and photosystem II (PSII) RCs, have been published in recent years. 13−18 …”

Section: Introductionmentioning

confidence: 99%

Cytochrome c Provides an Electron-Funneling Antenna for Efficient Photocurrent Generation in a Reaction Center Biophotocathode

Friebe¹,

Millo²,

Swainsbury

et al. 2017

ACS Appl. Mater. Interfaces

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The high quantum efficiency of photosynthetic reaction centers (RCs) makes them attractive for bioelectronic and biophotovoltaic applications. However, much of the native RC efficiency is lost in communication between surface-bound RCs and electrode materials. The state-of-the-art biophotoelectrodes utilizing cytochrome c (cyt c) as a biological wiring agent have at best approached 32% retained RC quantum efficiency. However, bottlenecks in cyt c-mediated electron transfer have not yet been fully elucidated. In this work, protein film voltammetry in conjunction with photoelectrochemistry is used to show that cyt c acts as an electron-funneling antennae that shuttle electrons from a functionalized rough silver electrode to surface-immobilized RCs. The arrangement of the two proteins on the electrode surface is characterized, revealing that RCs attached directly to the electrode via hydrophobic interactions and that a film of six cyt c per RC electrostatically bound to the electrode. We show that the additional electrical connectivity within a film of cyt c improves the high turnover demands of surface-bound RCs. This results in larger photocurrent onset potentials, positively shifted half-wave reduction potentials, and higher photocurrent densities reaching 100 μA cm–2. These findings are fundamental for the optimization of bioelectronics that utilize the ubiquitous cyt c redox proteins as biological wires to exploit electrode-bound enzymes.

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Section: Introductionmentioning

confidence: 99%

Cytochrome c Provides an Electron-Funneling Antenna for Efficient Photocurrent Generation in a Reaction Center Biophotocathode

Friebe¹,

Millo²,

Swainsbury

et al. 2017

ACS Appl. Mater. Interfaces

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“…The unique features of natural photosynthesis, especially its high quantum yield, are a source of inspiration for artificial solar fuel systems . Various biophotoelectrochemical strategies have been reported, which share their aim to exploit the high quantum yield of light harvesting proteins, such as photosystems I and II (PSI and PSII), but differ in their application, which ranges from biophotovoltaics, optobioelectronics to fuel production and water splitting . For the production of solar fuels, both the use of platinum, organometalic catalysts and redox enzymes have been explored.…”

Section: Introductionmentioning

confidence: 99%

Exploring Step‐by‐Step Assembly of Nanoparticle:Cytochrome Biohybrid Photoanodes

et al. 2017

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Coupling light‐harvesting semiconducting nanoparticles (NPs) with redox enzymes has been shown to create artificial photosynthetic systems that hold promise for the synthesis of solar fuels. High quantum yields require efficient electron transfer from the nanoparticle to the redox protein, a property that can be difficult to control. Here, we have compared binding and electron transfer between dye‐sensitized TiO2 nanocrystals or CdS quantum dots and two decaheme cytochromes on photoanodes. The effect of NP surface chemistry was assessed by preparing NPs capped with amine or carboxylic acid functionalities. For the TiO2 nanocrystals, binding to the cytochromes was optimal when capped with a carboxylic acid ligand, whereas for the CdS QDs, better adhesion was observed for amine capped ligand shells. When using TiO2 nanocrystals, dye‐sensitized with a phosphonated bipyridine Ru(II) dye, photocurrents are observed that are dependent on the redox state of the decaheme, confirming that electrons are transferred from the TiO2 nanocrystals to the surface via the decaheme conduit. In contrast, when CdS NPs are used, photocurrents are not dependent on the redox state of the decaheme, consistent with a model in which electron transfer from CdS to the photoanode bypasses the decaheme protein. These results illustrate that although the organic shell of NPs nanoparticles crucially affects coupling with proteinaceous material, the coupling can be difficult to predict or engineer.

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“…Therefore, by changing sizes or shapes of metallic nanoparticles, it is possible to tune the position of the plasmon resonance to match the optical spectra of emitters [9,21,26]. Additionally, the geometry of the hybrid photosynthetic structure can be tested and optimized for achieving the required functionality [27][28][29][30][31]. Within the infinite variety of metallic nanostructures, in the context of photosynthetic hybrid devices, Silver Island Film (SIF) [25] seems to be close to the optimal choice.…”

Section: Introductionmentioning

confidence: 99%

Silver Island Film for Enhancing Light Harvesting in Natural Photosynthetic Proteins

Kowalska

Szalkowski

Sulowska

et al. 2020

IJMS

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The effects of combining naturally evolved photosynthetic pigment–protein complexes with inorganic functional materials, especially plasmonically active metallic nanostructures, have been a widely studied topic in the last few decades. Besides other applications, it seems to be reasonable using such hybrid systems for designing future biomimetic solar cells. In this paper, we describe selected results that point out to various aspects of the interactions between photosynthetic complexes and plasmonic excitations in Silver Island Films (SIFs). In addition to simple light-harvesting complexes, like peridinin-chlorophyll-protein (PCP) or the Fenna–Matthews–Olson (FMO) complex, we also discuss the properties of large, photosynthetic reaction centers (RCs) and Photosystem I (PSI)—both prokaryotic PSI core complexes and eukaryotic PSI supercomplexes with attached antenna clusters (PSI-LHCI)—deposited on SIF substrates.

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Photosystem I Protein Films at Electrode Surfaces for Solar Energy Conversion

Cited by 66 publications

References 64 publications

Cytochrome c Provides an Electron-Funneling Antenna for Efficient Photocurrent Generation in a Reaction Center Biophotocathode

Cytochrome c Provides an Electron-Funneling Antenna for Efficient Photocurrent Generation in a Reaction Center Biophotocathode

Exploring Step‐by‐Step Assembly of Nanoparticle:Cytochrome Biohybrid Photoanodes

Silver Island Film for Enhancing Light Harvesting in Natural Photosynthetic Proteins

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