Photothermal stress triggered by near-infrared-irradiated carbon nanotubes up-regulates osteogenesis and mineral deposition in tooth-extracted sockets

Kajiya, Hiroshi; Katsumata, Yuri; Sasaki, Minako; Tsutsumi, Takashi; Kawaguchi, Minoru; Fukushima, Tadao

doi:10.3109/02656736.2015.1041430

Cited by 21 publications

(15 citation statements)

References 44 publications

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“…The results of cell viability experiments in the present study indicated that 45°C thermal treatment in vitro does not harm MC3T3 cells seriously, and stimulates ALP activity. Compared with the effects of osteogenesis in the previous study [42], those in the present study seemed to be weak. This is likely to be attributable to differences in temperature and the frequency of treatments between them.…”

Section: Discussioncontrasting

confidence: 99%

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Heat-stimuli-enhanced osteogenesis using clinically available biomaterials

et al. 2017

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A recent study reported that heat stress stimulates osteogenesis in an in vivo rat model using alginate gel and magnetite cationic liposomes. However, for clinical use, the efficacy for promoting osteogenesis needs to be investigated using clinically approved materials, and preferably with animals larger than rats. The aim of this study was to evaluate multiple heat stimuli-triggered osteogenesis in rat tibial defect models using already clinically applicable materials (Resovist® and REGENOS®) and determine the efficacy also in the rabbit. Fifty-eight rats and 10 rabbits were divided into two groups, respectively, with or without hyperthermia treatment at 45°C for 15 min. (hyperthermia; 20 rats once a week, 8 rats three times a week, 5 rabbits once a week, control; 30 rats and 5 rabbits). Micro-CT assessment at 4 weeks revealed that a significantly stimulated osteogenesis was observed in the once a week group of both rats and rabbits as compared to the control group (p = 0.018 and 0.036, respectively). In contrast, the three times a week group did not show enhanced osteogenesis. Histological examination and image analysis showed consistent results in which the area of mineralized bone formation in the once a week hyperthermia group was significantly increased compared with that in the control group at four weeks (rat; p = 0.026, rabbit; p = 0.031). Newly formed bone was observed in the grafted materials from the periphery toward the center, and more osteoclasts were found in the once a week group. Heat stress also induced enhanced alkaline phosphatase expression in cultured osteoblastic cells, MC3T3, in vitro (p = 0.03). On the other hand, heat stress had no obvious effects on chondrogenic differentiation using ATDC5 cells. Our study demonstrates that heat-stimuli with clinically applicable novel heating materials can promote significant osteogenesis, and may thus be a promising treatment option for diseases associated with bone defects.

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Section: Discussioncontrasting

confidence: 99%

“…On the other hand, Kajiya et al showed that everyday thermal treatment at 42°C for 10min. increased the activity of ALP in MC3T3 cells in a time-dependent manner compared with the non-thermal stress control [42]. Chen et al demonstrated the upregulated osteoblastic differentiation from MSCs with mild heat shock (41°C) [19].…”

Section: Discussionmentioning

confidence: 99%

Heat-stimuli-enhanced osteogenesis using clinically available biomaterials

et al. 2017

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“…4A), which suggested that 6 h at 5% 1 HZ stretch is the ideal condition to stimulate the osteogenic differentiation of BMSCs. Similar optimal force was also identified in photothermal stress-induced osteogenesis (37). These results will form the theoretical guidance for application during orthodontic tooth movement.…”

Section: Discussionsupporting

confidence: 67%

Expression of HIF‑1α in cycling stretch‑induced osteogenic differentiation of bone mesenchymal stem cells

Liu

et al. 2019

Mol Med Report

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During orthodontic treatment, mechanical force is applied to the teeth, and following a series of complex metabolism changes, the position of the teeth in the alveolar bone change. This process is closely associated with primitive bone mesenchymal stem cells (BMSCs), which may differentiate into osteoblasts precursor cell. A hypoxic microenvironment may be caused by orthodontic mechanical forces between the alveolar bone and the root. Hypoxia-inducible factor 1α (HIF-1α) is a specific receptor that adapts to a hypoxic environment. The present study was designed to investigate whether HIF-1α was involved in the osteoblastic differentiation of BMSCs induced by cyclic tensile stress. During this process, HIF-1α mRNA and protein expression were detected using a reverse transcription-quantitative polymerase chain reaction and western blotting. It was revealed that alkaline phosphatase activity increased in a time-dependent manner in three different stretching strength groups, which indicates that cyclic stretch promotes the osteogenic differentiation of BMSCs. The optimal force stage of osteogenesis was an unexpected discovery, which will provide theoretical guidance for selecting the most suitable orthodontic force for tooth movement in clinical orthodontic treatment. Most importantly, all experiments revealed that HIF-1α mRNA and protein were significantly increased following stretching treatment in BMSCs. It was therefore concluded that HIF-1α may be involved in BMSCs modulating osteogenic metabolism during exposure to cyclic stretch and a hypoxic microenvironment, which may prove useful for the reconstruction of a jaw during orthodontic treatment.

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“…In the present study, severe hyperthermia alone significantly suppressed RUNX2 expression, which declined to near normal levels of control after combination with berberine. It is worth mentioning that thermal stress induces the expression of alkaline phosphatase, RUNX2 and Osx in mouse osteoblast MC3T3-E1 cells (19). Osx was upregulated in all groups.…”

Section: Discussionmentioning

confidence: 87%

“…Overall, the present study showed that hyperthermia may induce differentiation of osteosarcoma cells. Kajiya et al (19) reported that photothermal stress promotes mineral deposition in enhanced tooth-extracted sockets. On the other hand, it has been reported that berberine activates RUNX2 by p38 mitogen-activated protein kinase, leading to increased expression of osteogenic marker genes involving osteocalcin and osteopontin (20).…”

Section: Discussionmentioning

confidence: 99%

Berberine Chloride and Hyperthermia Promote Osterix Expression and Suppress Cell Cycle Genes in Osteosarcoma Cells

Nashiry¹,

Al-Khateeb²,

Froemming³

2018

YJMS

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Objective: To investigate the effects of berberine chloride and hyperthermia on the expression of Osterix (Osx), Runt-related transcription factor 2 (RUNX-2), receptor activator of nuclear factor kappa-B ligand (RANKL), cyclin-dependent kinase 2 (CDK2), CDK4, interleukin (IL)-6 and IL-11 genes. Methods: Osteosarcoma cells (MG-63 cells) were treated with three different hyperthermia conditions for 1 h: two groups with mild, two with moderate and two with severe hyperthermia (at 39, 43 and 45 °C, respectively). Berberine chloride (80 µg/ ml) was used for treating one group of mild, moderate and severe hyperthermia (combination). All treated groups were recovered at 37°C for 24 h. Another exposure to hyperthermia (1 h) and recovery at 37°C for 3 h were applied. Gene expression levels were then determined using RT 2 PCR profiler arrays. Results: The expression of Osx was highly upregulated in all groups except in the groups treated with severe hyperthermia and mild hyperthermia in combination with berberine chloride. Mild hyperthermia alone induced a slight increase in the expression of RUNX2, whereas severe hyperthermia significantly suppressed RUNX2 levels. Berberine alone significantly induces the up-regulation of RANKL expression. On the other hand, CDK2 mRNA was downregulated in all groups. CDK4 was equally regulated in mild hyperthermia compared to the control group. However, the expression was significantly downregulated in other groups, especially in severe hyperthermia. IL-6 was significantly upregulated in berberine group and all groups of combinations, whereas mild and moderate hyperthermia significantly induced the expression of IL-11 mRNA. Conclusions: Hyperthermia and berberine chloride can promote osteosarcoma cells differentiation and suppresses cell cycle genes.

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Photothermal stress triggered by near-infrared-irradiated carbon nanotubes up-regulates osteogenesis and mineral deposition in tooth-extracted sockets

Abstract: Our novel CNT-based PTS up-regulated osteogenesis via activation of heat shock-related molecules, resulting in promotion of mineral deposition in enhanced tooth-extracted sockets.

Cited by 21 publications

References 44 publications

Heat-stimuli-enhanced osteogenesis using clinically available biomaterials

Heat-stimuli-enhanced osteogenesis using clinically available biomaterials

Expression of HIF‑1α in cycling stretch‑induced osteogenic differentiation of bone mesenchymal stem cells

Berberine Chloride and Hyperthermia Promote Osterix Expression and Suppress Cell Cycle Genes in Osteosarcoma Cells

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