Photothrombosis combined with thrombin injection establishes a rat model of cerebral venous sinus thrombosis

Chen, Chuanxiang; Wang, Qing; Gao, Yuyuan; Lu, Zhenyu; Cui, Xubo; Zheng, Tao; Liu, Y.; Li, X.; He, Xin; Zhang, X.; Li, Tingting

doi:10.1016/j.neuroscience.2015.08.020

Cited by 17 publications

(22 citation statements)

References 43 publications

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“…Moreover, the toxicity of ferric chloride itself could corrode the cerebral cortex, resulting in false positives. The pathophysiological process of thrombosis induced by the photochemical method was more in accordance with the clinical data, but arterial thrombosis could also be induced in the irradiation area [51].…”

Section: Discussionsupporting

confidence: 71%

“…Despite the various modeling methods, each model explained the pathophysiology of CVST from its respective perspectives. However, most models use invasive surgery, which can lead to iatrogenic injury of the brain parenchyma [7,10,12]. In addition, with uncontrollable spontaneous recanalization and few positive ndings, further research on therapies is limited [13].…”

Section: Introductionmentioning

confidence: 99%

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A Novel Rat Model for Cerebral Venous Sinus Thrombosis Matched Clinical Syndrome: Combination of Clinical Cases and Experimental Validation

Mu¹,

Lin²,

Xu³

et al. 2021

Preprint

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BackgroundCerebral venous sinus thrombosis (CVST) is a rare neurovascular disorder with highly variable manifestations and clinical course. To investigate its pathophysiology, an animal model with a properly matched clinic is needed. MethodsThis study consisted of clinical and animal studies. In the clinical section, eligible patients with CVST and healthy controls’ detailed data were recorded between January 1, 2016 and May 31, 2021. For the animal section, a novel rat model of CVST was established by inserting a water-swellable rubber into the superior sagittal sinus and applying imaging, histopathological, serological and behavioral tests to investigate the corresponding pathophysiological changes. A total of 19 CVST patients and 25 healthy controls were enrolled, and the data demonstrated the typical characteristics of venous infarction with elevated intracranial pressure, vasogenic edema, and secondary hemorrhage. A total of 80 rats were randomly divided into two groups: sham group (n = 40) and model group (n = 40). ResultsThe imaging results showed consistent characteristics of brain injury and venous morphology in the animal models and CVST patients. Histological and serological findings further illustrated the predominance of vasogenic edema as pathological change, while behavioral tests revealed anxiety and depression in model rats. ConclusionsWe established a novel CVST rat model with reproducible surgical methods and stabilization of pathological changes, and compared it to retrospective CVST patient data, proving that the model could almost reproduce the clinical syndromes. This model provides reliable support for the study of CVST pathophysiology and the evaluation of therapies.

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Section: Discussionsupporting

confidence: 71%

Section: Introductionmentioning

confidence: 99%

A Novel Rat Model for Cerebral Venous Sinus Thrombosis Matched Clinical Syndrome: Combination of Clinical Cases and Experimental Validation

Mu¹,

Lin²,

Xu³

et al. 2021

Preprint

View full text Add to dashboard Cite

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“…Only 1 patient achieved complete recanalization of the venous sinus; however, the majority of patients had a favorable prognosis, despite the low recanalization rate of venous sinus. This may be explained by the compensation of cortical venous flow and neuronal function restoration, which has been demonstrated in a novel CVST animal model ( 43 ). Therefore, systemic anticoagulant therapy is still an optimal option for treating CVST patients who present with superior neurological status.…”

Section: Discussionmentioning

confidence: 94%

Stent retriever thrombectomy combined with local thrombolytic therapy for cerebral venous sinus thrombosis: A case report

Chen

Wang

et al. 2017

Exp Ther Med

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The present study reports the effect of successful treatment of cerebral venous sinus thrombosis (CVST) with stent retriever thrombectomy combined with local thrombolytic therapy. Medical records of 29 patients were retrospectively analyzed following a diagnosis of CVST with magnetic resonance venography (MRV) or digital subtraction angiography (DSA). Systemic anticoagulation was the initial treatment in all patients following admission. In group A, stent retriever thrombectomy combined with local thrombolytic therapy was performed on 14 patients who met the criteria of endovascular therapy. Stent-assisted angioplasty was also performed when patients presented with venous sinus stenosis. A total of 15 patients in group B received systemic anticoagulant treatment. Subsequently, warfarin was administered orally for 3 to 12 months as a continuous anticoagulant therapy. International normalized ratio was monitored when patients were receiving anticoagulant therapy. Additionally, clinical presentation, decision to escalate therapy, recanalization, Glasgow Coma Scale, modified Rankin Scale (mRS) and the clinical outcome was assessed. A total of 14 patients (9 female patients, 5 male patients), with ages ranging from 17 to 57 years, met the criteria of endovascular therapy. The clinical symptoms of 12 patients had improved after receiving endovascular therapy and only 2 patients suffered from intracranial hemorrhage following the procedure. Complete recanalization of venous sinus was exhibited in 10/14 (71.4%) patients in group A when compared with 1/15 (6.7%) patients in group B. mRS were improved in the 12-month follow-up in groups A and B when compared with that at admission. In the present study, patients with acute CVST treated with stent retriever thrombectomy combined with local thrombolytic therapy had a favorable outcome. To conclude, the present study provides a treatment option in treating CVST, particularly for patients that present with evident cortical venous outflow stasis or deteriorate neurology, despite appropriate anticoagulant therapy.

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“…Moreover, the toxicity of ferric chloride itself can lead to corrosion of the cerebral cortex, resulting in false-positive identification of damage to the brain parenchyma. Although the pathophysiological process of thrombosis induced by the photochemical method was in greater accordance with the clinical data, it can also lead to arterial thrombosis in the irradiated area [ 37 ].…”

Section: Discussionmentioning

confidence: 89%

A novel rat model for cerebral venous sinus thrombosis: verification of similarity to human disease via clinical analysis and experimental validation

Lin

et al. 2022

J Transl Med

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Background Cerebral venous sinus thrombosis (CVST) is a rare neurovascular disorder with highly variable manifestations and clinical courses. Animal models properly matched to the clinical form of CVST are necessary for elucidating the pathophysiology of the disease. In this study, we aimed to establish a rat model that accurately recapitulates the clinical features of CVST in human patients. Methods This study consisted of a clinical analysis and animal experiments. Clinical data for two centres obtained between January 2016 and May 2021 were collected and analysed retrospectively. In addition, a Sprague–Dawley rat model of CVST was established by inserting a water-swellable rubber device into the superior sagittal sinus, following which imaging, histological, haematological, and behavioural tests were used to investigate pathophysiological changes. Principal component analysis and hierarchical clustering heatmaps were used to evaluate the similarity between the animal models and human patients. Results The imaging results revealed the possibility of vasogenic oedema in animal models. Haematological analysis indicated an inflammatory and hypercoagulable state. These findings were mostly matched with the retrospective clinical data. Pathological and serological tests further revealed brain parenchymal damage related to CVST in animal models. Conclusions We successfully established a stable and reproducible rat model of CVST. The high similarity between clinical patients and animal models was verified via cluster analysis. This model may be useful for the study of CVST pathophysiology and potential therapies.

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Photothrombosis combined with thrombin injection establishes a rat model of cerebral venous sinus thrombosis

Cited by 17 publications

References 43 publications

A Novel Rat Model for Cerebral Venous Sinus Thrombosis Matched Clinical Syndrome: Combination of Clinical Cases and Experimental Validation

A Novel Rat Model for Cerebral Venous Sinus Thrombosis Matched Clinical Syndrome: Combination of Clinical Cases and Experimental Validation

Stent retriever thrombectomy combined with local thrombolytic therapy for cerebral venous sinus thrombosis: A case report

A novel rat model for cerebral venous sinus thrombosis: verification of similarity to human disease via clinical analysis and experimental validation

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