Phototoxicity of Mesoporous TiO 2 + Gd Microbeads With Theranostic Potential

Kononenko, Veno; Imani, Roghayeh; Repar, Neža; Benčina, Metka; Lorenzetti, Martina; Erman, Andreja; Drobne, Damjana; Iglič, Aleš

doi:10.1016/bs.abl.2017.06.002

Cited by 3 publications

(5 citation statements)

References 48 publications

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“…In vitro cytotoxicity of TiO 2 @xGd NBs added to MG-63 cells in the absence of irradiation was tested and the cell viability results confirmed that TiO 2 @xGd NBs are fully biocompatible for all tested concentrations [8,145]. Furthermore, in vitro test results showed that attachment to cell surfaces and internalization was greater for TiO 2 @xGd NBs in comparison with un-doped TiO 2 NBs (Fig.…”

Section: The Role Of Membrane Vesiculation and Encapsulation In Cancementioning

confidence: 66%

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The role of membrane vesiculation and encapsulation in cancer diagnosis and therapy

Drab

Mesarec

Imani

et al. 2019

Advances in Biomembranes and Lipid Self-Assembly

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Introduction 160 2. Extracellular vesicles as diagnostic biomarkers for cancer 161 2.1 The fluid mosaic model of the membrane 164 2.2 Anisotropic orientational ordering and topological defects 165 2.3 Theoretical modeling of membrane vesiculation 166 3. Biocompatible nanostructured materials for cancer diagnostic and therapy 173 3.1 Photoactive nanoparticles for cancer treatment applications 174 4. Nanoparticle encapsulation 185 4.1 Monte Carlo simulations of particle encapsulation 186 5. Conclusions 189 Acknowledgements 190 References 190

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Section: The Role Of Membrane Vesiculation and Encapsulation In Cancementioning

confidence: 66%

“…The TiO 2 MBs were synthesized via the solvothermal method; obtained MBs were monodispersed spheres with a diameter of 500 AE 50 nm and comparatively rough surfaces (Fig. 6) [145].…”

Section: The Role Of Membrane Vesiculation and Encapsulation In Cancementioning

confidence: 99%

The role of membrane vesiculation and encapsulation in cancer diagnosis and therapy

Drab

Mesarec

Imani

et al. 2019

Advances in Biomembranes and Lipid Self-Assembly

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“…The amount of cellular proteins, which is proportional to the cell number, was evaluated by the Coomassie Blue (CB) assay [20]. CB dye binds to all cellular proteins, making cellular proteins amount evaluation possible by spectrophotometric measurement.…”

Section: Resultsmentioning

confidence: 99%

“…The same type of NPs that disturbed the lysosomal membranes (Magnéli-A, Magnéli-C, and TiO 2 -A; Figure 3a) also increased the cellular amounts of acid organelles in fully supplemented medium (shown by the increased neutral red fluorescence; Figure 2e), which is a known consequence of loading lysosomes with non-degraded material [43]. It is known that TiO 2 NPs exhibits high photocatalytic activity, leading to ROS photogeneration and oxidative disruption of lysosomal membranes [20,21]. Magnéli phase NPs, on the other hand, have no such photocatalytic activity (Figure S1), and the mechanism of their lysosomal disruptions remains unclear.…”

Section: Discussionmentioning

confidence: 99%

“…Bulk TiO 2 is suitable for numerous commercial applications including its use as a white pigment in paints, papers, and plastics, as well as other uses in cosmetics, medicine, and in the food industry. The nanostructured forms of TiO 2 have several desirable properties that can be used in sensors, solar cells, medical implants, and photodynamic therapy [15,16,17,18,19,20,21]. TiO 2 is a semiconductor, but its electrical conductivity is enhanced when it is converted to sub-stoichiometric Magnéli phase TiO x [22].…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Cytotoxicity Evaluation of the Magnéli Phase Titanium Suboxides (TixO2x−1) on A549 Human Lung Cells

Kononenko

Drobne

2019

IJMS

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The use of titanium suboxides, known as Magnéli phase TiOx, is expected to increase in the near future due to their desirable properties. In order to use Magnéli phase TiOx nanoparticles safely, it is necessary to know how nanoparticles interact with biological systems. In this study, the cytotoxicity of three different Magnéli TiOx nanoparticles was evaluated using human lung A549 cells and the results were compared with hazard data on two different TiO2 nanoparticles whose biological interactions have already been extensively studied. After A549 cells were exposed to nanoparticles, the metabolic activity was measured by the Resazurin assay, the amount of cellular proteins was measured by the Coomassie Blue assay, and lysosomal integrity was measured by the Neutral Red Uptake assay. In order to investigate possible modes of particle actions, intracellular Ca2+ level, reactive oxygen species (ROS) production, and photo-oxidative disruptions of lysosomal membranes were assessed. All experiments were performed in serum-containing and in serum-deprived cell culture mediums. In addition, the photocatalytic activity of Magnéli TiOx and TiO2 nanoparticles was measured. The results show that Magnéli TiOx nanoparticles increase intracellular Ca2+ but not ROS levels. In contrast, TiO2 nanoparticles increase ROS levels, resulting in a higher cytotoxicity. Although Magnéli TiOx nanoparticles showed a lower UV-A photocatalytic activity, the photo-stability of the lysosomal membranes was decreased by a greater extent, possibly due to particle accumulation inside lysosomes. We provide evidence that Magnéli TiOx nanoparticles have lower overall biological activity when compared with the two TiO2 formulations. However, some unique cellular interactions were detected and should be further studied in line with possible Magnéli TiOx application. We conclude that Magnéli phase nanoparticles could be considered as low toxic material same as other forms of titanium oxide particles.

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Nikotinski acetilholinski receptor kot farmakološka tarča pri pljučnem raku

Kononenko,

Bele,

Novak

et al. 2022

ABS

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Rak pljuč je zelo razširjena oblika raka z nizko stopnjo preživetja. Kajenje tobaka predstavlja glavni dejavnik tveganja za razvoj raka pljuč, saj v tobačnem dimu najdemo veliko rakotvornih snovi. Nikotin, ki sicer ni opredeljen kot rakotvoren, je glavna komponenta tobaka odgovorna za zasvojenost, poleg tega pa raziskave nakazujejo, da neodvisno od ostalih komponent tobaka v telesu sproži različne učinke, ki vplivajo na razvoj in napredovanje raka. Kot agonist nikotinskih acetilholinskih receptorjev (nAChR) nikotin spodbuja proliferacijo celic, preprečuje njihovo apoptozo, ter igra pomembno vlogo pri pospeševanju angiogeneze in zasevanju rakavih celic. Antagonisti nAChR, ki bi zavirali proliferacijo rakavih celic ter spodbujali njihovo apoptozo, predstavljajo velik terapevtski potencial. Trenutno je znanih le malo antagonistov nAChR, pri katerih je bila protirakava učinkovitost že raziskana, poleg tega pa po večini tudi niso dovolj selektivni ligandi za podvrste nAChR, ki se prekomerno izražajo v celicah pljučnega raka, zato je pričakovati škodljive stranske učinke. V izogib slednjim, se išče načine za ciljano dostavo antagonista nAChR do rakavih celic. Precej obetajo nanodostavni sistemi, ki omogočajo prednostni vnos aktivne učinkovine v celice raka. V našem članku predstavljamo najnovejše dosežke razvoja zdravil za zdravljenje pljučnega raka na osnovi antagonstov nAChR, dostavljenih na mesto delovanja s pomočjo nanodelcev.

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Phototoxicity of Mesoporous TiO 2 + Gd Microbeads With Theranostic Potential

Cited by 3 publications

References 48 publications

The role of membrane vesiculation and encapsulation in cancer diagnosis and therapy

The role of membrane vesiculation and encapsulation in cancer diagnosis and therapy

In Vitro Cytotoxicity Evaluation of the Magnéli Phase Titanium Suboxides (TixO2x−1) on A549 Human Lung Cells

Nikotinski acetilholinski receptor kot farmakološka tarča pri pljučnem raku

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