Phototropic H2 production by a newly isolated strain of Rhodopseudomonas palustris

Abstract

Rhodopseudomonas palustris TN1 was isolated from Songkhla Lake, Thailand. It phototrophically generates H(2) from the predominant volatile fatty acids (VFAs) produced from microbial dark-fermentations of palm oil milling effluent; yields from 20 mM butyrate, acetate and propionate were 4.7, 2.5, and 1.7 mol H(2) mol VFA(-1) with light efficiencies of 1.8, 1, and 0.2%, respectively. Optimum conditions were pH 7 and 3000 lux, although production was reduced by only 33% at 1000 lux. CO(2) evolution never exceeded 9 mmol mol VFA(-1).

Breakdown of food waste by anaerobic fermentation and non-oxygen producing photosynthesis using a photosynthetic bacterium

“…The isolate NW16 removed a greater percentage of the heavy metals than the isolate KMS24. Furthermore, a strain of Rhodopseudomonas palustris, TN1 isolated from Songkhla Lake in Southern Thailand was found to be an effective producer of hydrogen gas (Suwansaard et al, 2009;Suwansaard et al, 2010) from palm oil mill effluent. Rhodopseudomonas palustris, TN1 was also shown to be able to grow on VFAs (Volatile Fatty Acids) and will also grow on added acetate, butyrate, and propionate as carbon sources.…”

“…R. palustris grew very well between 1-7 day and after 7 day on day 10 of the experiment the cells had not grown any more than they had on day 7. R. palustris can grew on acetate, butyrate and propionate present in anaerobic fermentative treated palm oil milling effluent (Suwansaard et al, 2010). This is an important result because breakdown of grease balls and waste fat deposits are a major environmental problem and accumulation of organic matter as grease balls not only slows down breakdown of the oil, fat and grease but other organic matter are bound up with the grease.…”

“…Rhodopseudomonas strains are also capable of hydrogen gas production both anaerobically in the dark and photosynthetically using organic compounds as a hydrogen source (Hillmer and Gest, 1977;Mangels et al, 1986;Suwansaard et al, 2009;Panwichian et al,. 2009;Suwansaard et al, 2010). R. palustris can produce hydrogen gas from palm oil milling effluent which has a very high oil and fatty acid content (VFAs-Volatile Fatty Acids) (Suwansaard et al, 2009).…”

“…R. palustris can produce hydrogen gas from palm oil milling effluent which has a very high oil and fatty acid content (VFAs-Volatile Fatty Acids) (Suwansaard et al, 2009). Furthermore, a strain of R. spalustris, TN1 isolated from Songkhla Lake in Southern Thailand was found to be an effective producer of hydrogen gas (Suwansaard et al, 2009;Suwansaard et al, 2010) from palm oil mill effluent. …”

Efeitos dos produtos de hidrólise de materiais lignocelulósicos sobre a produção de H2 por fermentação

“…Esse processo tem como vantagem a possibilidade de utilização de diferentes resíduos e efluentes contendo carboidratos como potenciais substratos e pode utilizar amplo espectro de luz, porém, como nos métodos supracitados, também necessita de iluminação constante (SUWANSAARD et al, 2010 ;KAWAGOSHI et al, 2010).…”