Phyllanthus maderaspatensis, a dietary supplement for the amelioration of adriamycin-induced toxicity and oxidative stress in mice

Abstract

The effect of ethanol extract of Phyllanthus maderaspatensis (PME), a popular south Indian dietary supplement, was studied for its chemoprotective property on adriamycin (ADR)-induced toxicity and oxidative stress in mice. Adriamycin toxicity was evaluated biochemically by measuring the serum concentration of lactate dehydrogenase (LDH) and creatinine phosphokinase (CPK). Genotoxicity was evaluated by measuring the frequency of micronucleated polychromatic erythrocytes (MNPCEs) in bone marrow cells. Oxidative stress in the heart tissue was estimated by measuring the glutathione (GSH) levels in the homogenate. The treatment of mice with different doses of PME (400 mg/kg and 600 mg/kg body weight, p.o.,) for 7 days before the administration of a single i.p. dose of ADR (15 mg/kg) exhibited significant protection in a dose-dependent manner. The results clearly indicate that PME has a protective effect against ADR-induced toxicity, as revealed by the decrease in the concentrations of LDH, CPK, and the frequency of MNPCEs. The increased levels of GSH are indicative of the antioxidant property of PME.

Antinociceptive, genotoxic and histopathological study of Heliopsis longipes S.F. Blake in mice

“…It has been reported that daunorubicin induces severe chromosome damage by measuring sister chromatid exchanges (SCE) and micronuclei (MN). For this reason, it is used as a positive control in experimental studies (Bommu et al, 2008: Paniagua-Pérez et al, 2009. In contrast, HLEE produced neither genotoxic nor cytotoxic damage in the present study.…”

Clastogenicity of Beta-Caryophyllene in Mouse

“…8) Since the chemical has also been reported to induce severe chromosome damage in mice by measuring sister chromatid exchanges (SCE) and micronuclei (MN), it is used as a positive agent in experimental studies. 9,10) The results obtained in the assay with three administrations of BC are presented in Tables 3 and 4. The first table shows that the level of micronuclei induced by BC with the two low doses tested along the study was similar to the one detected in the control animals; in contrast, as regards the high dose we found an increase in the rate of MNPE from 48 to 96 h of the experiment, although without statistical significance.…”

Oxidative stress on cardiotoxicity after treatment with single and multiple doses of doxorubicin

“…Earlier cardiac myocytes necrosis has been detected in cell cultures 7 h after DOX exposure (0.5 mg/mL) 37 and in Wistar rats, 48 h after DOX treatment (25 mg/kg body weight). 38 Other studies have shown myofibrillar disarrangement 38 and decreased cardiac hydrophilic antioxidant defense (glutathione, 39 catalase, 40 superoxide dismutase, and glutathione peroxidase 40,41 ), after the first, 39 second, 41 or third 40 day of DOX injection in rodents. Clinical study identified significant reduction of plasma total hydrophilic antioxidant defense (TRAP, peroxyl radical-trapping antioxidant) at 8 h after the first adriamycin infusion in patients.…”