Phylogenetic Analyses of Proteins Coordinating G2 Size Control in Fission Yeast

Nagy, Zsófia; Medgyes-Horváth, Anna; Szalay, Csilla; Sipiczki, Matthias; Sveiczer, Ákos

doi:10.3311/ppch.14051

Cited by 3 publications

(4 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3 a). Duplication of WEE1 genes is not restricted to legumes and plants but has already been documented in metazoans and fungi 20 . Using only two plant sequences, Sorrel et al 4 found that the catalytic domain was conserved across animals and plants and that the two plants sequences grouped together.…”

Section: Introductionmentioning

confidence: 98%

Structural conservation of WEE1 and its role in cell cycle regulation in plants

Détain

Redecker

Leborgne-Castel

et al. 2021

Sci Rep

View full text Add to dashboard Cite

The WEE1 kinase is ubiquitous in plant development and negatively regulates the cell cycle through phosphorylations. However, analogies with the control of the human cell cycle by tyrosine- (Tyr-) phosphorylation of cyclin-dependent kinases (CDKs) are sometimes questioned. In this in silico study, we assessed the structural conservation of the WEE1 protein in the plant kingdom with a particular focus on agronomically valuable plants, the legume crops. We analyzed the phylogenetic distribution of amino-acid sequences among a large number of plants by Bayesian analysis that highlighted the general conservation of WEE1 proteins. A detailed sequence analysis confirmed the catalytic potential of WEE1 proteins in plants. However, some substitutions of an arginine and a glutamate at the entrance of the catalytic pocket, illustrated by 3D structure predictions, challenged the specificity of this protein toward the substrate and Tyr-phosphorylation compared to the human WEE1. The structural differences, which could be responsible for the loss of specificity between human and plants, are highlighted and suggest the involvement of plant WEE1 in more cell regulation processes.

show abstract

Section: Introductionmentioning

confidence: 98%

Structural conservation of WEE1 and its role in cell cycle regulation in plants

Détain

Redecker

Leborgne-Castel

et al. 2021

Sci Rep

View full text Add to dashboard Cite

show abstract

“…As a consequence, mitotic onset might happen only if the cell has reached a critical length. It is also noteworthy that some of these upstream regulators (like Wee1 and Cdc25) of Cdk in G2 phase are evolutionarily conserved, whereas others (like Pom1 and Cdr2) are present only in fission yeasts (Nagy, Medgyes‐Horváth, Szalay, Sipiczki, & Sveiczer, 2019; Sorrell et al, 2002). This may probably be a consequence of the special geometry (cylindrical form with constant diameter) of these yeast cells, which is common in prokaryotes, but very rare in eukaryotes.…”

Section: Introductionmentioning

confidence: 99%

Strongly oversized fission yeast cells lack any size control and tend to grow linearly rather than bilinearly

Nagy

Medgyes-Horváth

Vörös

et al. 2020

Yeast

Self Cite

View full text Add to dashboard Cite

During the mitotic cycle, the rod‐shaped fission yeast cells grow only at their tips. The newly born cells grow first unipolarly at their old end, but later in the cycle, the ‘new end take‐off’ event occurs, resulting in bipolar growth. Photographs were taken of several steady‐state and induction synchronous cultures of different cell cycle mutants of fission yeast, generally larger than wild type. Length measurements of many individual cells were performed from birth to division. For all the measured growth patterns, three different functions (linear, bilinear and exponential) were fitted, and the most adequate one was chosen by using specific statistical criteria, considering the altering parameter numbers. Although the growth patterns were heterogeneous in all the cultures studied, we could find some tendencies. In cultures with sufficiently wide size distribution, cells large enough at birth tend to grow linearly, whereas the other cells generally tend to grow bilinearly. We have found that among bilinearly growing cells, the larger they are at birth, the rate change point during their bilinear pattern occurs earlier in the cycle. This shifting near to the beginning of the cycle might finally cause a linear pattern, if the cells are even larger. In all of the steady‐state cultures studied, a size control mechanism operates to maintain homeostasis. By contrast, strongly oversized cells of induction synchronous cultures lack any sizer, and their cycle rather behaves like an adder. We could determine the critical cell size for both the G1 and G2 size controls, where these mechanisms become cryptic. TAKE AWAY Most individual fission yeast cells in steady‐state cultures grow bilinearly. In strongly oversized fission yeast cells, linear growth dominates over bilinear. Above birth length thresholds, both the G1 and G2 size controls become cryptic.

show abstract

“…Analysing the wee1-50 mutant is interesting, as cells of this mutant are small and have a generation time similar to WT, but have quite different distribution of cell cycle phases compared to WT [44,45]. The protein encoded by the wee1 gene is a highly conserved cell cycle regulator among eukaryotes [46,47]. The pom1 gene encodes an upstream regulator of the Wee1 protein, which is localized in the cell cortex with a decreasing spatial gradient from the poles to the middle of the cell [14,48].…”

Section: Growth Patterns In Different Cell Culturesmentioning

confidence: 99%

“…Moreover, a large number of cells are worth being involved in the analyses. It is noteworthy that the Pom1 protein is not conserved at all, but is unique to the genus Schizosaccharomyces [47].…”

Section: Growth Patterns In Different Cell Culturesmentioning

confidence: 99%

Cell Length Growth in the Fission Yeast Cell Cycle: Is It (Bi)linear or (Bi)exponential?

et al. 2021

Self Cite

View full text Add to dashboard Cite

Fission yeast is commonly used as a model organism in eukaryotic cell growth studies. To describe the cells’ length growth patterns during the mitotic cycle, different models have been proposed previously as linear, exponential, bilinear and biexponential ones. The task of discriminating among these patterns is still challenging. Here, we have analyzed 298 individual cells altogether, namely from three different steady-state cultures (wild-type, wee1-50 mutant and pom1Δ mutant). We have concluded that in 190 cases (63.8%) the bilinear model was more adequate than either the linear or the exponential ones. These 190 cells were further examined by separately analyzing the linear segments of the best fitted bilinear models. Linear and exponential functions have been fitted to these growth segments to determine whether the previously fitted bilinear functions were really correct. The majority of these growth segments were found to be linear; nonetheless, a significant number of exponential ones were also detected. However, exponential ones occurred mainly in cases of rather short segments (<40 min), where there were not enough data for an accurate model fitting. By contrast, in long enough growth segments (≥40 min), linear patterns highly dominated over exponential ones, verifying that overall growth is probably bilinear.

show abstract

Phylogenetic Analyses of Proteins Coordinating G2 Size Control in Fission Yeast

Cited by 3 publications

References 41 publications

Structural conservation of WEE1 and its role in cell cycle regulation in plants

Structural conservation of WEE1 and its role in cell cycle regulation in plants

Strongly oversized fission yeast cells lack any size control and tend to grow linearly rather than bilinearly

Cell Length Growth in the Fission Yeast Cell Cycle: Is It (Bi)linear or (Bi)exponential?

Contact Info

Product

Resources

About