Phylogenetic Analysis of Nematodes of the GenusPratylenchusUsing Nuclear 26S rDNA

Al-Banna, Luma; Williamson, Valerie M.; Gardner, Scott Lyell

doi:10.1006/mpev.1996.0381

Cited by 92 publications

(73 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…5 are aligned sequences of the D3 region of the LS 28S rDNA for P. arlingtoni, designated as 'arl', for P. convallariae as 'con', and for P. fallax as 'fal'. Also shown is the sequence for P. penetrans (Al-Banna et al, 1997) from the GenBank database that most nearly resembled our new sequences.…”

Section: Molecular Characterisationmentioning

confidence: 66%

See 1 more Smart Citation

Morphological and molecular characterisation of Pratylenchus arlingtoni n. sp., P.convallariae and P.fallax (Nematoda: Pratylenchidae)

Handoo¹,

Carta²,

Skantar³

2001

Nematol

View full text Add to dashboard Cite

Summary -Pratylenchus arlingtoni n. sp. from the rhizosphere of grasses Poa pratensis and Festuca arundinacea at Arlington National Cemetery, VA, USA is characterised by six to eight lines in the lateral eld, and pyriform to slightly overlapping pharyngeal glands. Morphological comparisons are made with lesion nematodes having similar morphometrics, six lateral lines, or crenate tail tips. Molecular sequences of the LS 28S rDNA were generated for the new species as well as P. fallax and P. convallariae. The new species differs by only 1% from identical sequences found in P. fallax and P. convallariae.

show abstract

Section: Molecular Characterisationmentioning

confidence: 66%

“…5). Positions are numbered where 1 corresponds to number 3324 of the Caenorhabditis elegans 28S rRNA gene (Ellis et al, 1986;Al-Banna et al, 1997). The number and position of nucleotide differences among the four taxa were noted.…”

Section: Alignmentmentioning

confidence: 99%

Morphological and molecular characterisation of Pratylenchus arlingtoni n. sp., P.convallariae and P.fallax (Nematoda: Pratylenchidae)

Handoo¹,

Carta²,

Skantar³

2001

Nematol

View full text Add to dashboard Cite

show abstract

“…However, existence of this new species creates grave doubt about the separation of Apratylenchoides and Pratylenchus as this species links the two genera with an aberrant orientation of the EGL that shifts from dorsal to ventral with a variable frequency. Cladistic parsimony analysis using molecular data showed many paraphyletic genera inside the Pratylenchidae, and Pratylenchus was not an exception (Al-Banna et al, 1997). Integration of the discrepancies between the monophyletic groups recognized by morphological and molecular data is the current challenge in nematode taxonomy, and the status of some generic characteristics should be subject to revision in the course of character examination that will properly reflect nematode phylogeny.…”

Section: Discussionmentioning

confidence: 99%

A new species of the genus Pratylenchus from chrysanthemum in Kyushu, western Japan (Nematoda: Pratylenchidae)

Mizukubo¹,

Sugimura²,

Uesugi³

2007

Jpn. J. Nematol.

View full text Add to dashboard Cite

A root-lesion nematode population collected from rhizospheres of Chrysanthemum morifolium in Kyushu Is., western Japan, is described as Pratylenchus kumamotoensis sp. n. The new species is characterized by its esophageal glandular lobe (EGL) inconsistently overlapping the intestine (dorsal, lateral and ventral overlap in varied frequencies), 2-3-annules in lip region, average spear length 14-15 µm, moderately distanced vulva (V = 76-77), and thin tail terminal cuticle (< 2 µm). The new species is distinguished from closely related P. coffeae, P. pseudocoffeae, P. loosi, P. jaehni, and Apratylenchoides homoglans. The new species was placed in the genus Pratylenchus because of the lack of clear differences with the genus Pratylenchus. However, an inconsistent overlap of the EGL in the new species creates grave doubt about the separation of Pratylenchus and

show abstract

“…The phylogenetic trees presented here are derived from alignments of the rDNA ITS and D3 sequences. These fast evolving regions have been found to provide phylogenetically informative data for closely related genera and species in a range of invertebrates, including nematodes (Adams et al, 1998;Al-Banna et al, 1997;Carta et al, 2001;Livaitis et al, 1994;Nguyen et al, 2001). However, it will be necessary to find other more polymorphic molecular markers to fully resolve the phylogenetic relationships between Panagrolaimus clades 1-3.…”

Section: Discussionmentioning

confidence: 99%

The anhydrobiotic potential and molecular phylogenetics of species and strains ofPanagrolaimus(Nematoda, Panagrolaimidae)

Shannon

Browne

Boyd

et al. 2005

Journal of Experimental Biology

View full text Add to dashboard Cite

SUMMARY Members of the genus Panagrolaimus are bacterial-feeding nematodes that occupy a diversity of niches ranging from Antarctic and temperate soils to terrestrial mosses. Some members of this genus are able to survive extreme desiccation by entering into a state of suspended animation known as anhydrobiosis. We have assembled a collection of Panagrolaimusspecies and strains and have investigated their anhydrobiotic phenotypes. Our data show that within the genus Panagrolaimus there is a continuum of strains ranging from those unable to survive exposure to low relative humidity(RH) without prior preconditioning at high RH (slow desiccation strategists),through strains that have limited ability to survive rapid desiccation but whose anhydrobiotic ability improves upon preconditioning, to strains such as P. superbus that can readily survive immediate exposure to severe desiccation (fast desiccation strategists). Using this panel of nematodes we investigated the effect of preincubation at high RH on the accumulation of trehalose and on the nematodes' anhydrobiotic potential. We found that there is a strong correlation between trehalose induction and anhydrobiotic survival in Panagrolaimus. Furthermore, the high trehalose levels observed in fully hydrated P. superbus (10% dry mass) suggest that constitutive expression of trehalose pre-adapts this fast dehydration strategist to combat desiccation. All the strains observed, regardless of survival rates, undertook both coiling and clumping, which has the effect of reducing surface area and slowing the rate of water loss during desiccation. Phylogenetic analyses were carried out to investigate whether the observed anhydrobiotic phenotypes were the result of convergent evolution or represented a single phylogenetic lineage. These analyses, derived from alignments of the rDNA ITS and D3 sequences, indicate that the strongly anhydrobiotic strains of Panagrolaimus form a single phylogenetic lineage, which is separate from the weakly anhydrobiotic strains. The weakly anhydrobiotic strains are also phylogenetically divergent from each other. Our data indicate that Panagrolaimus has the potential to be an excellent model system for the investigation of molecular aspects of nematode anhydrobiosis.

show abstract

Phylogenetic Analysis of Nematodes of the GenusPratylenchusUsing Nuclear 26S rDNA

Cited by 92 publications

References 23 publications

Morphological and molecular characterisation of Pratylenchus arlingtoni n. sp., P.convallariae and P.fallax (Nematoda: Pratylenchidae)

Morphological and molecular characterisation of Pratylenchus arlingtoni n. sp., P.convallariae and P.fallax (Nematoda: Pratylenchidae)

A new species of the genus Pratylenchus from chrysanthemum in Kyushu, western Japan (Nematoda: Pratylenchidae)

The anhydrobiotic potential and molecular phylogenetics of species and strains ofPanagrolaimus(Nematoda, Panagrolaimidae)

Contact Info

Product

Resources

About