Phylogenetic analysis of pandemic 2009 influenza A virus circulating in the South American region: genetic relationships and vaccine strain match

Goñi, Natalia; Moratorio, Gonzalo; Ramas, Viviana; Cóppola, Leticia; Chiparelli, Héctor; Cristina, Juan

doi:10.1007/s00705-010-0825-7

Cited by 8 publications

(8 citation statements)

References 40 publications

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“…Phylogenetic analysis of the HA 1 gene from 2009A/H1N1 isolates from Kashmir revealed similarities to 2009A/H1N1 clade 7. This is in agreement with recent results obtained in other parts of the world, where clade 7 was the most commonly detected clade, although cocirculation of clades 5, 6, and 7 has been observed in some parts of the world 16–18 . Amino acid substitutions in the HA protein characteristic for clade 7 (P83S, S203T and I321V) were identified in all Srinagar isolates, with two exception where I321I was present in two of the isolates.…”

Section: Discussionsupporting

confidence: 92%

“…Few of the strains from Srinagar clustered with the Asian strains, with A/India‐Srinagar/3/2010 showing similarities to (A/Quebec/144147/2009); A/India‐Srinagar/4/2010 and A/India‐Srinagar/7/2010 showing similarities to (A/EHIME/33/2010), and A/India‐Srinagar/1/2010, A/India‐Srinagar/2/2010, and A/India‐Srinagar/6/2010 showing similarities to (A/Daegu/1873/2009) (Figure 2). Unlike cocirculation of clades 5, 6, and 7, in some parts of India and South America, 16,18 all isolates from Srinagar clustered in clade 7 (Figure 2).…”

Section: Resultsmentioning

confidence: 97%

“…Amino acid substitutions in the HA protein characteristic for clade 7 (P83S, S203T and I321V) were identified in all Srinagar isolates, with two exception where I321I was present in two of the isolates. It is important to note that substitutions P83S and S203T are located in the antigenic sites E and D, respectively, whereas substitution I321V is located outside the major defined HA antigenic epitope 18 . None of these substitutions are found in the vaccine strain A/California/7/2009 25 .…”

Section: Discussionmentioning

confidence: 99%

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Pandemic and seasonal influenza viruses among patients with acute respiratory illness in Kashmir (India)

Koul

Mir

Bali

et al. 2011

Influenza and Other Respiratory Viruses

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Please cite this paper as: Koul PA., et al. (2011) Pandemic and seasonal influenza viruses among patients with acute respiratory illness in Kashmir (India). Influenza and Other Respiratory Viruses 5(6), e521–e527. Background With the emergence of pandemic influenza A (2009A/H1N1) virus in India, we sought to determine the prevalence and clinical presentations of seasonal and pandemic influenza viruses among acute respiratory illness (ARI) patients from Srinagar, a temperate climate area in northern India, during the peak winter season. Methods Combined throat and nasal swabs, obtained from 194 (108 male) presenting with ARI from January to March 2010 (Week 53‐week 10), were tested by RT‐PCR for influenza A and B, including 2009A/H1N1 viruses. HA1 gene of selected 2009A/H1N1‐positive samples was sequenced, and phylogenetic analysis was carried out. Results Twenty‐one (10·8%, age 15–80 years, median age 40 years) patients tested positive for influenza viruses: 13 (62%) for 2009A/H1N1 virus, 6 (28·5%) for seasonal influenza A (H3N2), and 2 (9·5%) for influenza B. Twelve of the 13 patients with 2009A/H1N1 presented with febrile ARI, and eight had associated comorbidities. All of the patients recovered. Phylogenetic analysis of HA gene (n = 8) revealed that all strains from Srinagar clustered in 2009A/H1N1 clade seven along with the other 2009A/H1N1 strains from India. Amino acid substitutions in the HA protein defining clade seven (P83S, S203T, and I321V) were found in almost all isolates from Srinagar. Conclusions Both seasonal and 2009A/H1N1 viruses appear to be associated with ARI in Srinagar. The 2009A/H1N1 in Srinagar is genetically similar to globally circulating clade 7 strains, with unique signature sequences in the HA gene. Further investigations into ascertain the role of these mutations in possible alteration of the virulence and transmissibility of the virus are needed.

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Section: Discussionsupporting

confidence: 92%

Section: Resultsmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

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Pandemic and seasonal influenza viruses among patients with acute respiratory illness in Kashmir (India)

Koul

Mir

Bali

et al. 2011

Influenza and Other Respiratory Viruses

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“…6 The Ca1 antigenic site in the majority of the Lebanese isolates had an S206T mutation, which has been reported as a characteristic mutation of a widespread H1N1 pdm strain. [13][14][15] We showed that this strain also had 2 amino acid mutations in NA: V42I and N248D. The phylogenetic history of these viruses suggested that these NA mutations evolved prior to the HA S206T mutation.…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity and antiviral drug resistance of pandemic H1N1 2009 in Lebanon

Zaraket

Kondo

Tabet

et al. 2011

Journal of Clinical Virology

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“…Sequences from the HA segment reproduce the phylogenetic topology for, the presumably more virulent, clade 6 and 7 viruses [17]. In South America, viruses from clades 5, 6 and 7 co-circulated [21]. Polymorphisms found in clade 6 strains have been identified and include the K-15E, P83S and Q293H mutations (ESH strain) [5,[13][14][15]17].…”

Section: Introductionmentioning

confidence: 78%

Detection of the Influenza A(H1N1)pdm09 Virus Carrying the K-15E, P83S and Q293H Mutations in Patients Who Have Undergone Bone Marrow Transplant

et al. 2014

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The 2009 pandemic influenza A(H1N1)pdm09 virus emerged and caused considerable morbidity and mortality in the third world, especially in Brazil. Although circulating strains of A(H1N1)pdm09 are A/California/04/2009-like (CA-04-like) viruses, various studies have suggested that some mutations in the viral hemagglutinin (HA) may be associated with enhanced severity and fatality. This phenomenon is particularly challenging for immunocompromised individuals, such as those who have undergone bone marrow transplant (BMT), because they are more likely to display worse clinical outcomes to influenza infection than non-immunocompromised individuals. We studied the clinical and viral aspects of post-BMT patients with confirmed A(H1N1)pdm09 diagnosis in the largest cancer hospital in Brazil. We found a viral strain with K-15E, P83S and Q293H polymorphisms in the HA, which is presumably more virulent, in these individuals. Despite that, these patients showed only mild symptoms of infection. Our findings complement the discovery of mild cases of infection with the A(H1N1)pdm09 virus with the K-15E, P83S and Q293H mutations in Brazil and oppose other studies that have linked these changes with increased disease severity. These results could be important for a better comprehension of the impact of the pandemic influenza in the context of BMT.

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Phylogenetic analysis of pandemic 2009 influenza A virus circulating in the South American region: genetic relationships and vaccine strain match

Cited by 8 publications

References 40 publications

Pandemic and seasonal influenza viruses among patients with acute respiratory illness in Kashmir (India)

Pandemic and seasonal influenza viruses among patients with acute respiratory illness in Kashmir (India)

Genetic diversity and antiviral drug resistance of pandemic H1N1 2009 in Lebanon

Detection of the Influenza A(H1N1)pdm09 Virus Carrying the K-15E, P83S and Q293H Mutations in Patients Who Have Undergone Bone Marrow Transplant

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