Phylogenetic analysis of recent isolates of classical swine fever virus from Colombia

Sabogal, Z. Y.; Mogollón, J. D.; Rincón, M. A.; Clavijo, Alfonso

doi:10.1016/j.virusres.2005.06.016

Cited by 28 publications

(18 citation statements)

References 13 publications

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“…It clearly reveals the emergence of 2.1. genotype of CSFV in pig populations of north-east India (Figures 1 and 2). Shifting from genotype 1 viruses, which predominated in the 1940s and 1950s, to genotype 2 in the 1980s has been reported in Europe (Paton et al 2000) and a similar shift was also recorded from China (Tu et al 2001), Columbia (Sabogal et al 2006) and Taiwan (Deng et al 2005). In a study, 103 of 110 CSFV isolates from 27 provinces covering 98% of Chinese territory fell into group 2 and subgroup 2.1.…”

Section: Discussionmentioning

confidence: 56%

Emergence of 2.1. subgenotype of classical swine fever virus in pig population of India in 2011

Rajkhowa

Hauhnar

Lalrohlua

et al. 2014

Veterinary Quarterly

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Background: Limited studies are available on molecular epidemiology of classical swine fever virus (CSFV) in India and are restricted to domestic pigs. These studies show the presence of 1.1. genotype. Hypothesis/objectives: The aim of the present study was to subgenotype four CSFV isolates, two each from the outbreaks of CSF in wild (Sus scrofa) and domestic pigs of Mizoram state, India, in 2011. Animals and methods: CSFV isolates were subjected to nucleotide sequencing in E2 and NS5B genomic regions. Phylogenetic analysis of the isolates in both genomic regions was carried out with 39 Indian isolates (4 isolates from the present study of Mizoram state and 35 isolates from the other states of India) and 57 reference sequences retrieved from the GenBank database. Two of the 39 isolates from India were collected from wild boar and were subgenotyped as 2.1. Out of 37 isolates from domestic pigs, only two were subgenotyped as 2.1. Results: The analysis revealed the emergence of 2.1. subgenotype of CSFV in both wild and domestic pigs in India. Conclusions and clinical importance:The isolates from domestic pigs of Mizoram state (CSF/MZ/KOL/73 and CSF/MZ/ AIZ/115) were grouped in genotype 1 and subgenotype 1.1., thus confirming that the source of CSF outbreaks in domesticated pigs in Mizoram was not from wild pigs. The current study forms an essential step for better understanding of the epidemiology of 2.1 subgroup as well as the movement and spread of the disease in India.

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Section: Discussionmentioning

confidence: 56%

Emergence of 2.1. subgenotype of classical swine fever virus in pig population of India in 2011

Rajkhowa

Hauhnar

Lalrohlua

et al. 2014

Veterinary Quarterly

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“…Phylogenetic analyses performed in different parts of the world confirmed that CSF virus isolates that differ by genetic typing seem to be characteristic for certain geographic regions (Bartak and Greiser-Wilke, 2000;Blacksell et al, 2005;Chen et al, 2008;Arce et al, 2005;Kamakawa et al, 2006;Li et al, 2006;Pereda et al, 2005;Sabogal et al, 2006;Stadejek et al, 1997;Vilcek et al, 1997).…”

Section: -140mentioning

confidence: 95%

Control and eradication of Classic Swine Fever in wild boar

2009

EFSA Journal

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SummaryClassical swine fever (CSF) is a disease that has been causing major socio-economic damages in the EU during the last decades. Although considerable progress has been made in the eradication and prevention of the disease, the threat for an epidemic still exists.The virus is endemic in the wild boar population of several member states (MS). Wild boar cannot be managed as domestic pigs. Hunting and vaccination have been tentatively used in order to stop transmission by reducing the number of susceptibles. Oral vaccination of wild boar with modified live vaccine based on the C-strain (the only suitable) is used; this vaccine does not allow serological differentiation between vaccinated and infected animals.The aim of the control measures for CSF in wild boar is to reduce the risk of transmission to domestic pigs, to prevent an "endemic phase evolution" or to reduce the endemic phase duration.In order to support and to improve the control and eradication measures as regards CSF in wild boar, EFSA was requested by the Commission to provide scientific advice on the efficacy of the available surveillance, hunting and vaccination measures to control and eradicate CSF in feral pig populations (wild boar), considering the possible use of new diagnostic tests and vaccines.In order to reply to the mandate data were collected from MS through two questionnaires (CSF vaccines, hunting practices and CSF vaccination in wild boar). Data were also extracted from the EU CSF wild boar data base, after requesting permission from the participating MS.Considering that the data received were rather limited and not comparable, the WG decided to use a model in order to fill the gaps in the available knowledge. For the evaluation of the efficacy of CSF control in wild boar a continuous metapopulation compartmental model developed in an EU research project (FP6-5015599-CSFVACCINE&WILDBOAR) was applied.Although the disease manifests in the same manner in both domestic pig and wild boar it is more difficult to identify in wild boar because clinical monitoring of these is hardly possible. Wild boar found dead constitutes the main alert sign of CSF. Hunters and gamekeepers should be instructed to report the finding of each dead wild boar to the competent authority at all times. In case of high-risk situations, a passive surveillance should be complemented by an active serological surveillance. The active sampling of wild boar is not as efficient as in domestic pigs considering that hunting is the sole practical system to obtain samples and that the aim of hunting is quite different.Consequently, the sample size is not controlled by authorities and fits rarely the aim of the survey in terms of detecting the presence of disease at a certain level. In addition, the interpretation of serological results is confounded by maternal derived antibodies (until the 6th month of age), vaccination and the sampling quality.There is no definition at EU level of spatial and temporal units for surveillance, neither for criteria defining a disease free wi...

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“…For a fast diagnostic approach, short 59-NTR (150 nt)-and E2 (190 nt)-encoding sequence fragments are usually used for sequencing and subsequent typing. Based on these fragments, several studies have been published with CSFV isolates from all over the world (Bartak & Greiser-Wilke, 2000;Biagetti et al, 2001;Blacksell et al, 2004;Cha et al, 2007;Jemersic et al, 2003;Li et al, 2006;Pereda et al, 2005;Sabogal et al, 2006;Stadejek et al, 1997;Tu et al, 2001;Vilcek et al, 1997;Vlasova et al, 2003). Regarding these fragments, extensive datasets are available at the CRL .…”

Section: Discussionmentioning

confidence: 99%

Molecular epidemiology of current classical swine fever virus isolates of wild boar in Germany

Leifer

Hoffmann

Höper

et al. 2010

Journal of General Virology

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Classical swine fever (CSF) has caused significant economic losses in industrialized pig production, and is still present in some European countries. Recent CSF outbreaks in Europe were mainly associated with strains of genogroup 2 (subgroup 2.3). Although there are extensive datasets regarding 2.3 strains, there is very little information available on longer fragments or whole classical swine fever virus (CSFV) genomes. Furthermore, there are no detailed analyses of the molecular epidemiology of CSFV wild boar isolates available. Nevertheless, complete genome sequences are supportive in phylogenetic analyses, especially in affected wild boar populations. Here, German CSFV strains of subgroup 2.3 were fully sequenced using two different approaches: (i) a universal panel of CSFV primers that were developed to amplify the complete genome in overlapping fragments for chain-terminator sequencing; and (ii) generation of a single full-length amplicon of the CSFV genome obtained by long-range RT-PCR for deep sequencing with next-generation sequencing technology. In total, five different strains of CSFV subgroup 2.3 were completely sequenced using these newly developed protocols. The approach was used to study virus spread and evolutionary history in German wild boar. For the first time, the results of our study clearly argue for the possibility of a long-term persistence of genotype 2.3 CSFV strains in affected regions at an almost undetectable level, even after long-term oral vaccination campaigns with intensive monitoring. Hence, regional persistence in wild boar populations has to be taken into account as an important factor in the continual outbreaks in affected areas.

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Phylogenetic analysis of recent isolates of classical swine fever virus from Colombia

Cited by 28 publications

References 13 publications

Emergence of 2.1. subgenotype of classical swine fever virus in pig population of India in 2011

Emergence of 2.1. subgenotype of classical swine fever virus in pig population of India in 2011

Control and eradication of Classic Swine Fever in wild boar

Molecular epidemiology of current classical swine fever virus isolates of wild boar in Germany

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