Phylogenetic analysis of Ukrainian seed-transmitted isolate of Soybean mosaic virus

Mіshchеnkо, Lidiya; Dunich, Alina; Shcherbatenko, I.S.

doi:10.7124/bc.00097d

Cited by 3 publications

(2 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is known that only few single-amino-acid changes near the C terminus of the CP of certain SMV strains led to the impossibility to seed transmission (Jossey et al, 2013). It was also found several single nucleotide variations (SNVs) in different regions of genome of seed-transmitted SMV (Jo et al, 2017;Mishchenko et al, 2018c). So, such substitution can be involved to the ability of these SMV isolates infect GM soybean.…”

Section: Resultsmentioning

confidence: 99%

Influence of Abiotic and Biotic Factors on Productivity of Transgenic Soybean and Molecular Properties of Disease Pathogen

Mishchenko¹,

Dashchenko²,

Dunich³

et al. 2019

AgricultForest

View full text Add to dashboard Cite

For the first time in Ukraine, it has been shown that transgenic soybean cv. Grimo and Monro is affected by various pathogens, including viral diseases. Negative effect of the Soybean mosaic virus (SMV)-infection on the productivity and yield structure of soybean plants is proved. Also, significant role for the cultivation of transgenic soybeans (Kyiv and Poltava regions, Ukraine) is revealed for growth conditions. Cultivation of the cv. Monro in different weather and climatic conditions has shown that the growth limiting factor is moisture. In 2018, harvest of soybean cv. Monro in farm "Mriya" in Kyiv region was 3.1 t/ha, but in the farm "Mir" in Poltava region it was almost twice lower. We analyzed the coefficient of significance of deviations of the agrometeorological regime in 2018 comparing with the average perennial metadata. It was 1.14-2.30 and demonstrated that the conditions in 2018 are very different comparing with the average perennial parameters and close to the rare. SMV named SGK-17 (Ac No MG940988), isolated from transgenic soybean plants cv. Monro from Kyiv region, was studied in the detail. Nucleotide and amino acid sequences of the SGK-17 coat protein gene region (430 nt) were compared with sequences of SMV isolates from different countries. SGK-17 has the highest identity level (97.9% nt and 97.2% aa) with isolates from China, Poland, Iran, USA, Ukraine and is belonging to the one cluster with them. Four unique substitutions in CP gene of SGK-17 are revealed, which can be involved in its ability to infect transgenic soybean.

show abstract

Section: Resultsmentioning

confidence: 99%

Influence of Abiotic and Biotic Factors on Productivity of Transgenic Soybean and Molecular Properties of Disease Pathogen

Mishchenko¹,

Dashchenko²,

Dunich³

et al. 2019

AgricultForest

View full text Add to dashboard Cite

show abstract

“…(Mishchenko et al, 2018a). Two unique amino acid substitutions (Ser → Cys and Lys → Ala) have been identified in the fragment of capsid protein gene of seed-transmitted SMV isolate named SKS-18 that may be involved in seed transmission of the SMV and other important functions of the viral infection cycle (Mishchenko et al, 2018b).…”

Section: Introductionmentioning

confidence: 99%

Seed-Borne and Seed-Transmitted Viral Diseases and Their Effect on Yield of Different Soybean Genotypes

Mіshchеnkо¹,

Dunich²,

MISHCHENKO³

et al. 2022

AgricultForest

View full text Add to dashboard Cite

In 21 st century soybean is a strategic legume for world agriculture. The key factor in increasing soybean production is the average yield growth, which in 2018 and 2021 in Ukraine was 3 t/ha as in the EU. From 2007 to 2017 the soybean yield in Ukraine was 2 t/ha; from 1997 to 2006 it was only 1 t/ha. Unlike, in the EU since 1999 the yield was 3 t/ha, and only in 2003, 2007 and 2012 it was 2 t/ha. For Ukraine not the least role in this situation is played by the reduction of quality of soybean sowing material due to seed infections. It is known that the quality of soybean seeds is significantly deteriorating due to infection by soybean mosaic virus (SMV), which circulates in all soybean growing regions worldwide. The aim of the study was to investigate SMV seed infection in sowing material and its effect on the yield of different soybean genotypes. DAS-ELISA showed SMV presence in a small seeds fraction of several soybean genotypes in both seedlings (embryos and cotyledons) and plants grown from this seeds. The weight of virus-infected seeds was 137 mg compared to healthy (204 mg). It was found that the percentage of seed-transmitted SMV infection is from 1.9 to 10.5% in some of investigated genotypes. It has been proven that seed spotting does not always indicate the presence of virus. Phylogenetic analysis of the SMV CP gene fragment was performed. Percent of nucleotide and amino acid sequence identity of Ukrainian SMV isolate with isolates from other countries was established.

show abstract

Formation of Cell and Tissue Barriers in Phaseolus vulgaris L. Ovules in a System of Antiviral Resistance

et al. 2020

View full text Add to dashboard Cite

Phylogenetic analysis of Ukrainian seed-transmitted isolate of Soybean mosaic virus

Cited by 3 publications

References 22 publications

Influence of Abiotic and Biotic Factors on Productivity of Transgenic Soybean and Molecular Properties of Disease Pathogen

Influence of Abiotic and Biotic Factors on Productivity of Transgenic Soybean and Molecular Properties of Disease Pathogen

Seed-Borne and Seed-Transmitted Viral Diseases and Their Effect on Yield of Different Soybean Genotypes

Formation of Cell and Tissue Barriers in Phaseolus vulgaris L. Ovules in a System of Antiviral Resistance

Contact Info

Product

Resources

About