Phylogenetic analysis of VP2 gene of canine parvovirus and comparison with Indian and world isolates

Kaur, Gurpreet; Chandra, Mudit; Dwivedi, P. N.

doi:10.4149/av_2016_01_106

Cited by 4 publications

(8 citation statements)

References 15 publications

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“…The capsid protein VP2 has been compared for different types of CPV types and it has been found that the various types of CPV differ in one or two amino acid sequences [12]. Therefore the researchers have cloned full length VP2 gene from the field isolates of CPV and sequence analyzed it and upon comparison with Indian and world isolates have observed regional genetic variation in CPV types [5]. Sequence analysis of the virus gene further indicated that the genomic organization of CPV was similar to feline panleukopenia virus (FPV) and the nucleotide and amino acid homologies were 98% and 99% for CPV and FPV, respectively [13].…”

Section: Resultsmentioning

confidence: 99%

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Detection of CPV 2a Antigen Type of Canine Parvovirus in the States of Punjab and Assam, India

Das

Kaur

Chandra

et al. 2019

CJAST

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Aim: Canine Parvovirus (CPV) is an emerging and re-emerging virus of canines. The study was undertaken to analyze VP2 gene of CPV in the isolates from dogs positive for CPV infection. Methods: The rectal swabs were collected from dogs suspected of CPV and subjected to PCR and nested PCR. The regions compared in the study were Punjab to represent north part of India and Assam to represent north-eastern part of India. The sequence analysis of VP2 gene of CPV was done using NCBI BLAST from the isolates which were positive for CPV by nested PCR. Further, phylogenetic analysis was done to understand the prevailing antigenic type of CPV from northern and north eastern part of India. Results: The sequence analysis revealed that all the sequences of VP2 gene from the samples had 98-99% homology with Canine Parvovirus and phylogenetic analysis revealed that CPV 2a antigenic type is circulating in both the regions selected in the study. Conclusions:The present study revealed that CPV 2a is circulating in the regions of Punjab and Assam part of India. Further sequence analysis of VP2 gene from more number of field isolates can throw better light on the prevailing antigenic type of CPV in various parts of India.

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Section: Resultsmentioning

confidence: 99%

“…Since the emergence of CPV-2 from feline panleukopenia virus in 1978, many variants of CPV 2 have evolved worldwide within a short span of time. The antigenic types of CPV prevalent worldwide are CPV2a, CPV 2b, CPV 2c, New CPV2a, New CPV 2b [3,4,5,6,7,8]. The antigenic types of CPV vary only in their VP2 gene and that too only 0.1% i.e.…”

Section: Introductionmentioning

confidence: 99%

Detection of CPV 2a Antigen Type of Canine Parvovirus in the States of Punjab and Assam, India

Das

Kaur

Chandra

et al. 2019

CJAST

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“…CPV-2c has been found mainly in South American and European countries [ 19 ]. In Africa, the Middle East, Asia and Australia, CPV-2b and CPV-2a are the predominant variants, while CPV-2c seems to be absent [ 11 , 13 , 17 , 32 , 36 , 37 ]. In Europe, there seems to be a more equal spreading of the three variants [ 10 , 19 ], which could be related to geographical position and a different commercial flows of dogs.…”

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confidence: 99%

“…As already reported, CPV can be detected in the brain in experimental and natural infections [ 9 , 30 ]. The 12 CPV-2b strains account for about 44% of those examined, and they formed a separate cluster in the phylogenetic tree, indicating regional genetic variation of CPV-2 strains [ 17 ]. Our results showed that all currently known strains of CPV are circulating in northern Sardinia but that the new CPV-2b is the more widespread variant.…”

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confidence: 99%

First molecular characterization of canine parvovirus strains in Sardinia, Italy

et al. 2017

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Canine parvovirus type 2 (CPV-2) is responsible of acute hemorrhagic gastroenteritis in young dogs. CPV-2 emerged in 1978 in the USA, but new antigenic types, CPV-2a, 2b and 2c, have completely replaced the original type. In this study, we analyzed 81 animals collected in Sardinia, Italy. The VP2 sequence analysis of 27 positive samples showed that all antigenic CPV-2 types are circulating. CPV-2b seems to be the most widespread variant, followed by CPV-2a. Furthermore, 12 CPV-2b strains displayed further amino acid substitutions and formed a separate cluster in a phylogenetic tree, indicating regional genetic variation.

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“…The length of the amplified product with about 1300 bp correlated with SCARB2 gene. Gene comparison by BLAST (Kaur et al, 2016) showed that the amplified product was in 99% homologous with SCARB2 gene from GenBank (BT0069391) (Fig. 2).…”

Section: Amplification Of Scarb2 Genementioning

confidence: 96%

Prokaryotic expression and identification of scavenger receptor B2

Xu¹,

Lin²,

Wang³

et al. 2018

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There is still no effective clinical antiviral drug against human enterovirus 71 (EV71) infection, which causes hand, foot and mouth disease (HFMD) in children. Scavenger receptor class B member 2 (SCARB2) is an important receptor of EV71 as it plays a vital role in the early steps of viral infection. In this study, recombinant SCARB2 protein was expressed and purified in a prokaryotic expression system, and was identified by western blot with a monoclonal antibody and mass spectrometry analysis. Detection of the sera from mice immunized with the recombinant SCARB2 protein using ELISA and western blot showed good immunogenicity of the recombinant protein. Furthermore, in the neutralization test cytopathic effect was significantly decreased when EV71 was incubated with the immune sera before infection. In summary, the SCARB2 protein was expressed successfully, and the immune sera showed obvious antiviral effect against EV71. This study provides useful information about the interaction mechanism between SCARB2 and EV71, and is also helpful for further clinical treatment research of HFMD.

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Phylogenetic analysis of VP2 gene of canine parvovirus and comparison with Indian and world isolates

Cited by 4 publications

References 15 publications

Detection of CPV 2a Antigen Type of Canine Parvovirus in the States of Punjab and Assam, India

Detection of CPV 2a Antigen Type of Canine Parvovirus in the States of Punjab and Assam, India

First molecular characterization of canine parvovirus strains in Sardinia, Italy

Prokaryotic expression and identification of scavenger receptor B2

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