Phylogenetic analysis of β-xylanase SRXL1 of Sporisorium reilianum and its relationship with families (GH10 and GH11) of Ascomycetes and Basidiomycetes

Álvarez-Cervantes, Jorge; Díaz‐Godínez, Gerardo; Mercado-Flores, Yuridia; Gupta, Vijai Kumar; Anducho-Reyes, Miguel Angel

doi:10.1038/srep24010

Cited by 21 publications

(10 citation statements)

References 69 publications

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“…Although not analyzed in formal phylogenies, Chytridiomycota and Dikarya share predicted xyloglucanases 57,58 , further evidence that early osmotrophs digested the streptophyte cell walls. Lignin is restricted to land plants, and an expansion of peroxidase enzymes needed for lignin decomposition is credited with contributing to the diversification of Basidiomycota 3 .…”

Section: [H1] Introductionmentioning

confidence: 99%

Genomic and fossil windows into the secret lives of the most ancient fungi

et al. 2020

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Fungi have crucial roles in modern ecosystems as decomposers and pathogens, and they engage in a variety of mutualistic associations with other organisms, especially plants. They have a lengthy geological history, and there is an emerging understanding of their impact on the evolution of Earth systems on a large scale. In this Review, we focus on the roles of fungi in the establishment and early evolution of land and freshwater ecosystems. Today, questions of evolution over deep time are informed by discoveries of new fossils and evolutionary analysis of new genomes. Inferences can be drawn from evolutionary analysis by comparing the genes and genomes of fungi with the biochemistry and development of their plant and algal hosts. We then contrast this emerging picture against evidence from the fossil record to develop a new, integrated perspective on the origin and early evolution of fungi.

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Section: [H1] Introductionmentioning

confidence: 99%

Genomic and fossil windows into the secret lives of the most ancient fungi

et al. 2020

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“…In silico screening of sequence databases provides a powerful methodology for the search of enzymes with specific properties [ 25 , 26 ]. In this work, we have undertaken a bioinformatics analysis of family GH11 of glycoside hydrolases, that includes one of the major of ensemble of xylanases [ 27 ], aiming to the identification of extremophilic enzymes active at strongly alkaline pH and high temperature. This type of analysis is less laborious and allows wider-range hunts than experimental approaches based on cloning and screening of large number of genes [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

In silico screening and experimental analysis of family GH11 xylanases for applications under conditions of alkaline pH and high temperature

Talens-Perales

Sánchez-Torres

Marín-Navarro

et al. 2020

Biotechnol Biofuels

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Background Xylanases are one of the most extensively used enzymes for biomass digestion. However, in many instances, their use is limited by poor performance under the conditions of pH and temperature required by the industry. Therefore, the search for xylanases able to function efficiently at alkaline pH and high temperature is an important objective for different processes that use lignocellulosic substrates, such as the production of paper pulp and biofuels. Results A comprehensive in silico analysis of family GH11 sequences from the CAZY database allowed their phylogenetic classification in a radial cladogram in which sequences of known or presumptive thermophilic and alkalophilic xylanases appeared in three clusters. Eight sequences from these clusters were selected for experimental analysis. The coding DNA was synthesized, cloned and the enzymes were produced in E. coli. Some of these showed high xylanolytic activity at pH values > 8.0 and temperature > 80 °C. The best enzymes corresponding to sequences from Dictyoglomus thermophilum (Xyn5) and Thermobifida fusca (Xyn8). The addition of a carbohydrate-binding module (CBM9) to Xyn5 increased 4 times its activity at 90 °C and pH > 9.0. The combination of Xyn5 and Xyn8 was proved to be efficient for the saccharification of alkali pretreated rice straw, yielding xylose and xylooligosaccharides. Conclusions This study provides a fruitful approach for the selection of enzymes with suitable properties from the information contained in extensive databases. We have characterized two xylanases able to hydrolyze xylan with high efficiency at pH > 8.0 and temperature > 80 °C.

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“…Catalytic amino acids and enzymatic mechanism are conserved, presenting a domain for catalysis of 250-450 amino acids. From the biochemical point of view, most of them have high molecular weight though there are reports of low molecular weight enzymes [16]. The values of their pI are generally alkaline (8.0-9.5), however, some also have acid values and all of them sustain the same three-dimensional structure.…”

Section: Classification Of Xylanasesmentioning

confidence: 99%

Xylanase and Its Industrial Applications

Basit¹,

Jiang²,

Rahim³

2021

Biotechnological Applications of Biomass

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Lignocellulosic biomass is a renewable raw material. Industrial interest with new technology has grown to take advantage of this raw material. Different microbial enzymes are treated with biomass to produce the desired products under ideal industrial conditions. Xylanases are the key enzymes that degrade the xylosidic linkages in the xylan backbone of the biomass, and commercial enzymes are categorized into different glycoside hydrolase families. Thermophilic microorganisms are an excellent source of thermostable enzymes that can tolerate the extreme conditions of industrial processing. Thermostability of xylanases from thermophilic microorganisms has given the importance for a specific activity at elevated temperatures and distinction due to biochemical properties, structure, and mode of action. Optimized xylanases can be produced through genetic engineering: a novel xylanase is isolated from an extreme environment and then genetically modified to improve suitability for industrial contexts. Recombinant protein techniques have made it possible to engineer and express thermostable xylanases in bacteria, yeasts, and filamentous fungi. We will discuss the biotechnological potential of xylanases from thermophilic microorganism and the ways they are being optimized and expressed for industrial applications.

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Phylogenetic analysis of β-xylanase SRXL1 of Sporisorium reilianum and its relationship with families (GH10 and GH11) of Ascomycetes and Basidiomycetes

Cited by 21 publications

References 69 publications

Genomic and fossil windows into the secret lives of the most ancient fungi

Genomic and fossil windows into the secret lives of the most ancient fungi

In silico screening and experimental analysis of family GH11 xylanases for applications under conditions of alkaline pH and high temperature

Xylanase and Its Industrial Applications

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