Phylogenetic, Carbendazim Sensitivity and Mycotoxin Genotype Analyses of <i>Fusarium graminearum</i> Complex Species Isolated from Wheat Fusarium Head Blight in China

Xuli, Wang; Y, Cui; Feng, Fan; Song, Yuanyuan; Ren, Jingping; Meng, Qinglin; Xu, Weijun; Jiang, Lijing

doi:10.1111/j.1439-0434.2009.01662.x

Cited by 13 publications

(5 citation statements)

References 13 publications

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“…F. meridionale was reported in Sichuan, Yunnan and Hubei on wheat and barley, but similar to our study, it was typically found at low frequencies (Wang et al, 2010;Yang et al, 2008;Zhang et al, 2012). This contrasts with reports where F. meridionale was the predominant species on maize in Nepal and Northern Argentina (Desjardins and Proctor, 2011;Sampietro et al, 2012).…”

Section: Discussionsupporting

confidence: 56%

Crops are a main driver for species diversity and the toxigenic potential ofFusarium isolates in maize ears in China

Zhang

Brankovics

Luo

et al. 2016

WMJ

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In recent years increasing demands and the relatively low-care cultivation of the crop have resulted in an enormous expansion of the acreage of maize in China. However, particularly in China, Fusarium ear rot forms an important constraint to maize production. In this study, we showed that members of both the Fusarium fujikuroi species complex (FFSC) and the Fusarium graminearum species complex are the causal agents of Fusarium ear rot in the main maize producing areas in China. Fumonisin producing Fusarium verticillioides was the most prevalent species, followed by fumonisin producing Fusarium proliferatum and 15-acetyldeoxynivalenol producing F. graminearum. Both Fusarium temperatum and Fusarium boothii were identified for the first time in the colder regions in China, extending their known habitats to colder environments. Mating type analysis of the different heterothallic FFSC species, showed that both types co-occur in each sampling site suggestive of the possibility of sexual recombination. Virulence tests with F. boothii (from maize) and F. graminearum from maize or wheat showed adaptation to the host. In addition, F. graminearum seems to outcompete F. boothii in wheat-maize rotations. Based on our findings and previous studies, we conclude that wheat/maize rotation selects for F. graminearum, while a wheat/rice rotation selects for F. asiaticum. In contrast, F. boothii is selected when maize is cultivated without rotation. A higher occurrence of F. temperatum is observed on maize in colder climatological regions in China, while Fusarium meridionale seems restricted to mountain areas. Each of these species has their characteristic mycotoxin profile and deoxynivalenol and fumonisin are the potential threats to maize production in Northern China.

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Section: Discussionsupporting

confidence: 56%

Crops are a main driver for species diversity and the toxigenic potential ofFusarium isolates in maize ears in China

Zhang

Brankovics

Luo

et al. 2016

WMJ

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“…These results were in agreement with the previous study in this region [ 43 ], which showed that the percentage of carbendazim resistance isolates was higher in F. asiaticum. Wang et al [ 55 ] documented that colonies of F. asiaticum developed normally at a carbendazim concentration of 5.0 μg/mL, indicating that F. asiaticum was highly tolerant to carbendazim. In this text, all F. graminearum s. str.…”

Section: Resultsmentioning

confidence: 99%

Genetic Relationships, Carbendazim Sensitivity and Mycotoxin Production of the Fusarium Graminearum Populations from Maize, Wheat and Rice in Eastern China

Qiu

Shi

2014

Toxins

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Members of the Fusarium graminearum species complex (FGSC) are important pathogens on wheat, maize, barley, and rice in China. Harvested grains are often contaminated by mycotoxins, such as the trichothecene nivalenol (NIV) and deoxynivalenol (DON) and the estrogenic mycotoxin zearalenone (ZEN), which is a big threat to humans and animals. In this study, 97 isolates were collected from maize, wheat, and rice in Jiangsu and Anhui provinces in 2013 and characterized by species- and chemotype-specific PCR. F. graminearum sensu stricto (s. str.) was predominant on maize, while most of the isolates collected from rice and wheat were identified as F. asiaticum. Fusarium isolates from three hosts varied in trichothecene chemotypes. The 3-acetyldeoxynivalenol (3ADON) chemotype predominated on wheat and rice population, while 15ADON was prevailing in the remaining isolates. Sequence analysis of the translation elongation factor 1α and trichodiene synthase indicated the accuracy of the above conclusion. Additionally, phylogenetic analysis suggested four groups with strong correlation with species, chemotype, and host. These isolates were also evaluated for their sensitivity to carbendazim and mycotoxins production. The maize population was less sensitive than the other two. The DON levels were similar in three populations, while those isolates on maize produced more ZEN. More DON was produced in carbendazim resistant strains than sensitive ones, but it seemed that carbendazim resistance had no effect on ZEN production in wheat culture.

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“…Fungal diseases of plants cause huge losses in food production. Fusarium head blight (FHB) causes reduced grain yield and quality and kernel contamination with mycotoxins such as deoxynivalenol, nivalenol, T-2 toxin and zearalenone, which are harmful to livestock and threaten human food safety (Wang et al, 2010). In China, Fusarium graminearum is the main cause of FHB; for over 30 years it has been controlled largely by the application of carbendazim (Chen et al, 2007a).…”

Section: Introductionmentioning

confidence: 99%

Antifungal screening of endophytic fungi fromGinkgo bilobafor discovery of potent anti-phytopathogenic fungicides

Xiao

et al. 2013

FEMS Microbiol Lett

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Many endophytic fungi have been found to synthesize bioactive compounds to defend host plants against pathogenic organisms. Here we performed anti-fungal bioassay of 80 endophytic fungi isolated from Ginkgo biloba. Fifteen endophytes were active against at least one of the selected fungi, Fusarium graminearum, Sclerotinia sclerotiorum and Phytophthora capsici, using the agar diffusion method. The most bioactive strain CDW7 was identified as Chaetomium globosum by microscopic examination and ITS rRNA gene sequence data. Culture broth of CDW7 diluted 3-fold completely inhibited the mycelial growth and conidia germination of F. graminearum in vitro. Therefore, Fusarium head blight, a common disease in wheat and barley associated with Fusarium spp., was used to test the anti-phytopathogenic activity in vivo. The fermentation broth of CDW7 resulted in a protective efficacy of 54.9% and curative efficacy of 48.8%. Followed by a bioassay-guided approach, 1,2-benzenedicarboxaldehyde-3,4,5-trihydroxy-6-methyl (flavipin) was isolated and demonstrated to significantly inhibit the growth of several plant-pathogenic fungi, especially F. graminearum with an EC(50) value of 0.73 μg mL(-1) comparable to the commonly used fungicide carbendazim, indicating that it could be used as a fungicide or as a lead compound of new fungicides.

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Phylogenetic, Carbendazim Sensitivity and Mycotoxin Genotype Analyses of Fusarium graminearum Complex Species Isolated from Wheat Fusarium Head Blight in China

Cited by 13 publications

References 13 publications

Crops are a main driver for species diversity and the toxigenic potential ofFusarium isolates in maize ears in China

Crops are a main driver for species diversity and the toxigenic potential ofFusarium isolates in maize ears in China

Genetic Relationships, Carbendazim Sensitivity and Mycotoxin Production of the Fusarium Graminearum Populations from Maize, Wheat and Rice in Eastern China

Antifungal screening of endophytic fungi fromGinkgo bilobafor discovery of potent anti-phytopathogenic fungicides

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