Phylogenetic conservatism of mycoparasitism and its contribution to pathogen antagonism

Barge, Edward G.; Leopold, Devin R.; Rojas, Alejandro; Vilgalys, Rytas; Busby, Posy E.

doi:10.1111/mec.16436

Cited by 9 publications

(3 citation statements)

References 70 publications

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“…Clearly, this opportunity requires an assessment of whether these useful roles can be simultaneously performed by single selected strains. The recent observation that phylogenetically diverse species of Cladosporium display concurrent effects supports the conjecture that these functional traits are rooted in ancestry in this genus, providing a favorable indication in this respect [241].…”

Section: Discussionmentioning

confidence: 74%

Cladosporium—Insect Relationships

Nicoletti,

Russo,

Becchimanzi

2024

JoF

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The range of interactions between Cladosporium, a ubiquitous fungal genus, and insects, a class including about 60% of the animal species, is extremely diverse. The broad case history of antagonism and mutualism connecting Cladosporium and insects is reviewed in this paper based on the examination of the available literature. Certain strains establish direct interactions with pests or beneficial insects or indirectly influence them through their endophytic development in plants. Entomopathogenicity is often connected to the production of toxic secondary metabolites, although there is a case where these compounds have been reported to favor pollinator attraction, suggesting an important role in angiosperm reproduction. Other relationships include mycophagy, which, on the other hand, may reflect an ecological advantage for these extremely adaptable fungi using insects as carriers for spreading in the environment. Several Cladosporium species colonize insect structures, such as galleries of ambrosia beetles, leaf rolls of attelabid weevils and galls formed by cecidomyid midges, playing a still uncertain symbiotic role. Finally, the occurrence of Cladosporium in the gut of several insect species has intriguing implications for pest management, also considering that some strains have proven to be able to degrade insecticides. These interactions especially deserve further investigation to understand the impact of these fungi on pest control measures and strategies to preserve beneficial insects.

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Section: Discussionmentioning

confidence: 74%

Cladosporium—Insect Relationships

Nicoletti,

Russo,

Becchimanzi

2024

JoF

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“…kit or CTAB) DNA extraction methods and suitable for cultures, fresh and dried specimens, environmental samples and archival DNA extracts. Using multiplex PCR to simultaneously amplify multiple loci and pooling samples for PacBio long‐read HTS (Barge et al., 2022; Chen et al., 2015) is a cost‐effective method compared to Sanger sequencing, allowing sequencing of up to six loci from multiple samples. Our pooled barcoding approach to DNA sequencing is more economical than Sanger sequencing, especially as PacBio sequencing has increased in reliability and fidelity (Runnel et al., 2022; Tedersoo et al., 2021).…”

Section: Discussionmentioning

confidence: 99%

PacBio high‐throughput multi‐locus sequencing reveals high genetic diversity in mushroom‐forming fungi

Swenie,

Looney,

et al. 2023

Molecular Ecology Resources

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Multi‐locus sequence data are widely used in fungal systematic and taxonomic studies to delimit species and infer evolutionary relationships. We developed and assessed the efficacy of a multi‐locus pooled sequencing method using PacBio long‐read high‐throughput sequencing. Samples included fresh and dried voucher specimens, cultures and archival DNA extracts of Agaricomycetes with an emphasis on the order Cantharellales. Of the 283 specimens sequenced, 93.6% successfully amplified at one or more loci with a mean of 3.3 loci amplified. Our method recovered multiple sequence variants representing alleles of rDNA loci and single copy protein‐coding genes rpb1, rpb2 and tef1. Within‐sample genetic variation differed by locus and taxonomic group, with the greatest genetic divergence observed among sequence variants of rpb2 and tef1 from corticioid Cantharellales. Our method is a cost‐effective approach for generating accurate multi‐locus sequence data coupled with recovery of alleles from polymorphic samples and multi‐organism specimens. These results have important implications for understanding intra‐individual genomic variation among genetic loci commonly used in species delimitation of fungi.

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“…Endophytes have emerging mutualistic roles in planta in host protection against pests and pathogens, e.g., by antagonistic bioactive secondary metabolite production, by mycoparasitism of adverse pathogens, and by occupying an in planta niche that becomes then unavailable for invasion of pathogens. Fungal endophytes may confer abiotic stress tolerance and some promote plant growth by plant hormone production (Adnan et al 2022 ; Barge et al 2022 ; Eberl et al 2019 ; Jia et al 2020 ; Rabiey et al 2019 ; Rodriguez et al 2009 ; Zanne et al 2020 ). Others are latent and turn as opportunists into a pathogenic lifestyle once host tissues become stressed or when they senesce (Hardoim et al 2015 ; Rabiey et al 2019 ) or they are latent decomposers awaiting the host death as a resource for plant material consumption (Parfitt et al 2010 ).…”

Section: Fungal Communities Associated With Treesmentioning

confidence: 99%

Applying molecular and genetic methods to trees and their fungal communities

Müller

Kües

Budde

et al. 2023

Appl Microbiol Biotechnol

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Forests provide invaluable economic, ecological, and social services. At the same time, they are exposed to several threats, such as fragmentation, changing climatic conditions, or increasingly destructive pests and pathogens. Trees, the inherent species of forests, cannot be viewed as isolated organisms. Manifold (micro)organisms are associated with trees playing a pivotal role in forest ecosystems. Of these organisms, fungi may have the greatest impact on the life of trees. A multitude of molecular and genetic methods are now available to investigate tree species and their associated organisms. Due to their smaller genome sizes compared to tree species, whole genomes of different fungi are routinely compared. Such studies have only recently started in forest tree species. Here, we summarize the application of molecular and genetic methods in forest conservation genetics, tree breeding, and association genetics as well as for the investigation of fungal communities and their interrelated ecological functions. These techniques provide valuable insights into the molecular basis of adaptive traits, the impacts of forest management, and changing environmental conditions on tree species and fungal communities and can enhance tree-breeding cycles due to reduced time for field testing. It becomes clear that there are multifaceted interactions among microbial species as well as between these organisms and trees. We demonstrate the versatility of the different approaches based on case studies on trees and fungi. Key points • Current knowledge of genetic methods applied to forest trees and associated fungi. • Genomic methods are essential in conservation, breeding, management, and research. • Important role of phytobiomes for trees and their ecosystems.

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Phylogenetic conservatism of mycoparasitism and its contribution to pathogen antagonism

Cited by 9 publications

References 70 publications

Cladosporium—Insect Relationships

Cladosporium—Insect Relationships

PacBio high‐throughput multi‐locus sequencing reveals high genetic diversity in mushroom‐forming fungi

Applying molecular and genetic methods to trees and their fungal communities

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