Phylogenetic diversity of intra-amoebal legionellae as revealed by 16S rRNA gene sequence comparison

Birtles, Richard J.; Rowbotham, T. J.; Raoult, Didier; Harrison, Timothy G.

doi:10.1099/13500872-142-12-3525

Cited by 88 publications

(79 citation statements)

References 17 publications

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“…The phenetic characteristics of the LLAP strains studied, including their growth and cultural characteristics, biochemical reactions, CFA profiles and serological reactions are in agreement with previous inferences from phylogenetic analyses (Adeleke et al, 1996 ;Birtles et al, 1996). The degree of 16S rRNA sequence similarity between LLAPs and validly described members of the genus Legionella was found to be consistent with that between the existing members of the genus.…”

Section: A a Adeleke And Otherssupporting

confidence: 72%

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Legionella drozanskii sp. nov., Legionella rowbothamii sp. nov. and Legionella fallonii sp. nov.: three unusual new Legionella species.

Adeleke¹,

Fields²,

Benson³

et al. 2001

International Journal of Systematic and Evolutionary Microbiology

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Section: A a Adeleke And Otherssupporting

confidence: 72%

“…Genomic DNA was extracted from LLAP strains and amplified using PCR. The 16S rRNA gene sequences were then determined and analysed as described previously (Adeleke et al, 1996 ;Birtles et al, 1996).…”

Section: Strainsmentioning

confidence: 99%

Legionella drozanskii sp. nov., Legionella rowbothamii sp. nov. and Legionella fallonii sp. nov.: three unusual new Legionella species.

Adeleke¹,

Fields²,

Benson³

et al. 2001

International Journal of Systematic and Evolutionary Microbiology

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“…It has been proposed that LLAPs should not all be placed in the single species L. Zytica, but should be split into at least five species [17,20]. Whilst some of the ISR patterns obtained in this study showed correlation with the proposed new species, the agreement was not absolute (personal unpublished observations).…”

Section: Discussioncontrasting

confidence: 45%

“…Strains of legionellae were cultured on buffered charcoal yeast extract agar [19] at 30" or 37°C in a moist chamber or, in the case of legionella-like amoebal pathogens (LLAPs), their amoebal host [16, 17, 201. Non-type strains of legionellae used in this study were divided into two sets: dataset I (52 strains) comprising well characterised strains and dataset I1 (46 strains) comprising recent clinical and environmental isolates. Strains assigned to dataset I were available either from type culture collections (NCTC or ATCC), or had been the subject of phylogenetic studies, such as 16s rRNA (gene) sequence analysis [16,17,20,21], DNA restriction fragment length polymorphism (RFLP) analysis [ l l ] , or DNA:DNA homology [18]; the identity of these strains was taken to be 'true'. Strains assigned to dataset I1 were identified in the RSIL by standard phenotypic techniques, including growth requirements, biochemical characteristics and serological tests with hyperimmune rabbit antisera [6, 71, or genotypic RFLP analysis [ l l ] .…”

Section: Bacterial Strains and Culture Conditionsmentioning

confidence: 99%

An evaluation of intergenic rRNA gene sequence length polymorphism analysis for the identification of Legionella species

Fry¹,

Harrison²

1998

Journal of Medical Microbiology

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There are currently more than 40 species of Legionella and the identification of most of these by standard methods is technically difficult. The aim of this study was to assess the suitability of a previously published PCR-based method of identifying Legionella spp. Intergenic 16s-23s rDNA spacer regions were amplified with primers complementary to conserved regions of the rRNA genes. Following electrophoretic separation of the products, data analyses were performed with the Taxotron'lt software package.Computer-assisted analysis (with an empirically derived error tolerance of 3 YO) could differentiate only 26 of the 43 strains (representing 43 species), with the remaining 17 species clustering into four groups (group I, comprising 10 species; group 11, three species; group 111, two species and group IV, two species). Analysis of well-characterised 'non-type' strains of some Legionella spp. (e.g., from type culture collections) resulted in patterns distinct from the corresponding type strain in most cases. Furthermore, recent isolates (identified by conventional methods) were identified by this PCR method to the presumed correct species (or species group) in only a minority of cases. Well characterised strains and recent isolates of Legionella showed heterogeneity within many species. This intra-species variation severely limits the usefulness of the method for the identification of isolates. However, logical typing within such species.

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“…This would suggest that the relationship between the pink colony-forming bacterium and H. circularisquama is optional. In contrast, intracellular bacteria of some ciliates 8,9) and amoebae 3) could not be cultivated in any of the media. These relationships were essential for the bacteria.…”

Section: Discussionmentioning

confidence: 99%

Effects of Harmful Dinoflagellate Heterocapsa circularisquama Cells on the Growth of Intracellular Bacteria.

Maki

Imai

2001

Microb. Environ.

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The red tide dinoflagellate Heterocapsa circularisquama Horiguchi has intracellular bacteria. The isolation and cultivation of these bacteria were attempted for three-clonal strains of H. circularisquama (HY9423, HA92-1, and HU9433) using various culture media containing cell components of the host alga prepared by homogenization and heat killing, in addition to different organic materials (peptone and extracts). Only one intracellular bacterium of the bacterial population UBb in H. circularisquama strain HU9433 was successfully cultivated on many kinds of culture media. The intracellular bacterial populations of Yb and Ab in the algal clones HY9423 and HA92-1, respectively, could not grow in the culture media used. The presence of live algal cells is thought to be essential for the growth and survival of the bacterial populations Yb and Ab. These results indicate that the degree of dependence of the intracellular bacteria on the host algal cells differs greatly among the algae-bacteria sets of strains.

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Phylogenetic diversity of intra-amoebal legionellae as revealed by 16S rRNA gene sequence comparison

Cited by 88 publications

References 17 publications

Legionella drozanskii sp. nov., Legionella rowbothamii sp. nov. and Legionella fallonii sp. nov.: three unusual new Legionella species.

Legionella drozanskii sp. nov., Legionella rowbothamii sp. nov. and Legionella fallonii sp. nov.: three unusual new Legionella species.

An evaluation of intergenic rRNA gene sequence length polymorphism analysis for the identification of Legionella species

Effects of Harmful Dinoflagellate Heterocapsa circularisquama Cells on the Growth of Intracellular Bacteria.

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