Phylogenetic diversity of procaryotae and their contribution to excess sludge demineralization in a gravel contact oxidation reactor

Abstract

Phylogenetic diversity of procaryotae and their function to excess sludge demineralization in a gravel contact oxidation reactor (GCOR) were studied by constructing a 16SrRNA library and PCR-based molecular surveys of environmental 16SrRNA genes. The sequence analysis of 16SrRNA genes indicated that granular bacterial communities were composed of Proteobacteria, Bacteroidetes, Nitrobacteraceae, Low G+C and the Unclassified bacteria group. The dominant members were β-, γ-Proteobacteria. Different kinds of bacteria which contributed to excess sludge decrement were associated with uncoupled metabolism, diverse bacterial interactive behaviour and domination of slow growers.