Phylogenetic identification of epibiotic bacteria possessing antimicrobial activities isolated from red algal species of Japan

Abstract

We investigated the diversity of epibiotic bacteria possessing antimicrobial activity isolated from nine species of red algae, and identified their phylogenetic position. For the isolation of epibiotic bacteria, nine species of red algae, Pachymeniopsis lauceolata, Plocamium telfairiae, Gelidium amansii, Chondrus oncellatus, Grateloupia filicina, Ceramium kondoi, Lomentaria catenata, Schizymenia dubyi and Porphyra yezoensis, were collected from the intertidal zone of Awaji Island, Japan. In total 92 bacteria were collected from the above red algal species. Primary screening results using disc diffusion assay revealed that 33% of bacteria possess antibacterial activity. Ten bacteria that showed high antibacterial activity were further studied for their ability to inhibit a set of fouling bacteria, some luminescent Vibrio and Photobacterium species and a panel of pathogenic bacteria. In general, the inhibitory activities were high against fouling and luminescent bacteria, while low against various pathogenic bacteria tested. These results suggest that some epibiotic bacteria have adapted to defend their position in their surface environment through the production of antibacterial metabolites giving defense against a broad spectrum of bacterial competitors. The phylogenetic analysis using 16 S rRNA sequences identified 7 of the 10 strains as belonging to the genus Bacillus, and other strains each 1 belonging to genus Microbacterium, Psychrobacter, and Vibrio species.

Antidiatom and antibacterial activity of epiphytic bacteria isolated from Ulva lactuca in tropical waters

“…Although several studies have investigated the antibacterial activity of algal-associated bacteria (Egan et al 2000;Dobretsov and Qian 2002;Kanagasabhapathy et al 2008;Silva-Aciares and Riquelme 2008;Penesyan et al 2009;Nylund et al 2010), diatom-related studies are relatively limited (Dobretsov and Qian 2002;Silva-Aciares and Riquelme 2008). Remarkably, we found that antidiatom activity against C. fusiformis was common amongst the bacteria isolated in the current study, with 80% of the bacterial isolates expressing the activity (Table 2).…”

“…While antibacterial activity of surface-associated bacteria is well established (Rao et al 2005;Dobretsov et al 2006;Egan et al 2008;Kanagasabhapathy et al 2008;Nylund et al 2010;Penesyan et al 2010), antidiatom activity has been less investigated (Dobretsov and Qian 2002;Kang et al 2005; Silva-Aciares and Riquelme 2008). The latter might be due to the inherent, technical challenges of performing antidiatom assays.…”

Jania rubens-associated bacteria: molecular identification and antimicrobial activity

“…Kanagasabhapathy et al (2006) have isolated B. pumilus and other Bacillus species from different brown alga. Kanagasabhapathy et al (2008) investigated antimicrobial activity of epiphytic bacteria from several red algae and found that the highest activity was produced by certain Bacillus species especially B. cereus and B. pumilus. Our results are in agreement with these studies since B. pumilus was found active against S. aureus, Vibrio spp.…”

“…Recently, Kanagasabhapathy et al (2008) identified epibiotic bacteria of nine non-calcareous species of red algae from Japanese waters with antimicrobial activities. Other studies concerned the bacterial biota on crustose coralline algae (Lewis et al 1985;Johnson et al 1991; Barott et al 2011).…”

Bioreduction of Acetophenone Derivatives by Red Marine Algae Bostrychia radicans and B. tenella, and Marine Bacteria Associated

“…associated with interdital marine alga [33]. Recently, red algae species yielded marine bacteria of the Bacillus genus [34].…”