Phylogenetic relationship and characterization of the complete mitochondrial genome of the black citrus aphid,Aphis aurantii(Hemiptera: Aphididae)

Abstract

The black citrus aphid Aphis aurantii is a major pest of citrus and tea plants. In this study, we determined the complete mitochondrial genome (mitogenome) of A. aurantii. The mitogenome was 15,469 bp in length, consisting of 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, 2 ribosomal RNA (rRNA) genes, and a non-coding control region. Gene order of A. aurantii was highly conserved and identical to most other previously sequenced Aphididae. Initiation codons ATT, ATA, and ATG were identified in eight, three, and two PCGs, respectively, while stop codon TAA was found in 11 genes except for 2 genes (cox1 and nad4) which use incomplete stop codon T-. Phylogenetic analysis showed the topology (Pemphigidae þ (Hormaphididae þ (Greenideidae þ Aphididae))) within Aphidoidea, and A. aurantii was more closely related to Aphis craccivora than to Aphis gossypii. ARTICLE HISTORY

Transcriptomic Profiling of Various Developmental Stages of Aphis Aurantii to Provide a Genetic Resource for Gene Expression and SSR Analysis

“…Moreover, functional studies of the new insecticide targets in aphids were also conducted in these aphids (Ye et al, 2019 ; Shang et al, 2020a ). However, most previously published papers have focused on the ecology (Alizadeh Kafeshani et al, 2018 ; Guidolin and Consoli, 2018 ; Li et al, 2019 ), control (Aslam et al, 2015 ; Gholamzadeh-Chitgar and Pourmoradi, 2017 ), and mitochondrial genome (Wang et al, 2019 ) in tea aphid Ap. aurantii .…”

Population Genetics of the Black Citrus Aphid Aphis aurantii (Hemiptera, Aphididae) in China

“…The partial mitochondrial cytochrome c oxidase I (cox1) genes of some A. aurantii specimens were sequenced from Guangxi Province in southern China (Wang and Qiao, 2009), and apparent intraspecific genetic diversity was found within A. aurantii. The mitochondrial genome of A. aurantii from Guangxi Province was sequenced (Wang et al, 2019) and the phylogeny of Aphidoidea was rebuilt, revealing the close relationship between A. aurantii and Aphis craccivora Koch, 1854. A recent study sequenced the mitochondrial genome of A. aurantii from Sichuan Province and constructed the phylogeny of Aphididae (Pu et al, 2020), supporting the close relationship of A. aurantii and other aphids in Aphis.…”

The complete mitochondrial genome of Aphis gossypii Glover, 1877 (Hemiptera: Aphididae) isolated from Leonurus japonicus in Korea

“… 2017 ), Aphis aurantia (MN397939; Wang Y et al. 2019 ), Aphis citricidus (NC_043903; Wei et al. 2019 ), Schizaphis graminum (NC_006158; Thao et al.…”