Phylogenetic relationship of Chinese pangolin (<i>Manis pentadactyla aurita</i>) revealed by complete mitochondrial genome

Hua, Yan; Wang, Jiao; An, Fuyu; Xu, Jinqian; Zhang, Han; He-xiang, GU

doi:10.1080/23802359.2020.1772693

Cited by 4 publications

(4 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We also obtained 1,773 single-copy orthologous coding sequences comprising 1,872,840 nucleotides from the genomes. In addition, 169 mitogenomic sequences with an average length of 14,919 bp were obtained from the 138 genomic sequences and 31 published sequences ( SI Appendix , Table S3 ) ( 14 , 26 – 31 ).…”

Section: Resultsmentioning

confidence: 99%

Genomic analysis reveals a cryptic pangolin species

Gu,

Wu,

Yang

et al. 2023

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Eight extant species of pangolins are currently recognized. Recent studies found that two mitochondrial haplotypes identified in confiscations in Hong Kong could not be assigned to any known pangolin species, implying the existence of a species. Here, we report that two additional mitochondrial haplotypes identified in independent confiscations from Yunnan align with the putative species haplotypes supporting the existence of this mysterious species/population. To verify the new species scenario we performed a comprehensive analysis of scale characteristics and 138 whole genomes representing all recognized pangolin species and the cryptic new species, 98 of which were generated here. Our morphometric results clearly attributed this cryptic species to Asian pangolins ( Manis sp.) and the genomic data provide robust and compelling evidence that it is a pangolin species distinct from those recognized previously, which separated from the Philippine pangolin and Malayan pangolin over 5 Mya. Our study provides a solid genomic basis for its formal recognition as the ninth pangolin species or the fifth Asian one, supporting a new taxonomic classification of pangolins. The effects of glacial climate changes and recent anthropogenic activities driven by illegal trade are inferred to have caused its population decline with the genomic signatures showing low genetic diversity, a high level of inbreeding, and high genetic load. Our finding greatly expands current knowledge of pangolin diversity and evolution and has vital implications for conservation efforts to prevent the extinction of this enigmatic and endangered species from the wild.

show abstract

Section: Resultsmentioning

confidence: 99%

Genomic analysis reveals a cryptic pangolin species

Gu,

Wu,

Yang

et al. 2023

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…In addition, the nucleotide composition of the mitogenome was slightly A + T biased, which is similar to that of the other pangolins (Suppl. material 1 ) ( Du Toit et al 2017 , Hua et al 2020 ). These results also confirmed the A + T bias, which has been reported in several other mammalian species ( Dou et al 2016 ).…”

Section: Resultsmentioning

confidence: 99%

“…Thus, the subspecies status of the Chinese pangolin remains to be clarified with genetic evidence, including the nominal Formosan pangolin in Taiwan. However, the taxonomic status of all three subspecies has not been well resolved by genetic evidence ( Wu et al 2007 , Hua et al 2020 , Wu et al 2020 ). This impedes regional conservation and potential reintroduction, as well as illegal trade traces and law enforcement.…”

Section: Introductionmentioning

confidence: 99%

Complete mitochondrial genome of Manis pentadactyla pentadactyla (Mammalia: Pholidota), an endemic subspecies of Chinese pangolin: mitogenome characterisation and phylogenetic implications

Sun¹,

Huang²,

Tseng³

et al. 2021

BDJ

View full text Add to dashboard Cite

The Chinese pangolin Manis pentadactyla is critically endangered because of over-exploitation and illegal trafficking and includes three subspecies. However, the taxonomic status of the three subspecies of the Chinese pangolin has not been well resolved, which impedes regional conservation and illegal trade traces. In this study, the complete mitogenome sequence of M. p. pentadactyla, an endemic subspecies of the Chinese pangolin in Taiwan, was determined. The complete mitogenome of M. p. pentadactyla is 16,570 base pairs (bp) in length with 13 protein-coding genes (PCG), 23 transfer RNAs (tRNAs), two ribosomal RNAs and a 1164 bp control region. The overall base composition of the genome showed a slight A + T bias (59.9%), positive AT skew (0.1515) and negative GC skew (-0.3406), which is similar to that of other pangolins. All PCGs started with a typical ATN codon and all tRNAs were typical cloverleaf-shaped secondary structures, except for tRNA-Ser(GCU). Phylogenetic analysis indicated a monophyletic relationship for M. p. pentadactyla and M. p. aurita and was monophyletic for M. p. pentadactyla, but paraphyletic for M. p. aurita. The paraphyly of M. p. aurita resulted from an incomplete lineage sorting. This study enriched the mitogenome database of the Chinese pangolin and the molecular information obtained should be very useful for future research on mitogenome evolution and genetic diversification in M. pentadactyla.

show abstract

“…The Sunda pangolin ( Manis javanica ) and Chinese pangolin ( Manis pentadactyla ) are both listed as endangered species [ 1 , 2 , 3 ] and are among the most trafficked mammals in the world [ 4 ]. All pangolin species are classified as a National Level I Protected Animals in China [ 5 ] and are listed as Critically Endangered on the International Union for Conservation of Nature (IUCN) Red List of Threatened Species [ 6 , 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Comparison of Venous Blood Gas and Biochemical Parameters in Sunda Pangolin (Manis javanica) and Chinese Pangolin (Manis pentadactyla) before and after Isoflurane Anesthesia

Yan

et al. 2023

Animals

Self Cite

View full text Add to dashboard Cite

Venous blood gas analytes are commonly examined in animals, and the results may be important when evaluating the overall health status of an animal. Pangolins are critically endangered mammals, and there is limited information on their physiological reference values in the literature. The aim of this study was to analyze venous blood gas and biochemical parameters before and during isoflurane anesthesia in wild healthy Sunda and Chinese pangolins. The results obtained showed that the blood gas index trends of the two pangolin species before and after isoflurane anesthesia were the same. After anesthesia, the partial pressure of carbon dioxide (pCO2), partial pressure of oxygen (pO2), total carbon dioxide (CO2), mean blood bicarbonate (HCO3−), extracellular fluid compartment (BEecf) base excess and the mean blood glucose (Glu) levels of both pangolin species showed a significant increase compared to the pre-anesthesia period. In contrast, the mean blood potassium (K+), lactate (Lac) and mean blood pH levels were significantly lower. No significant differences in the mean blood sodium (Na+) or blood ionized calcium (iCa) levels were observed during anesthesia. This study is important for future comparisons and understanding the health status of this endangered species.

show abstract

Phylogenetic relationship of Chinese pangolin (Manis pentadactyla aurita) revealed by complete mitochondrial genome

Cited by 4 publications

References 5 publications

Genomic analysis reveals a cryptic pangolin species

Genomic analysis reveals a cryptic pangolin species

Complete mitochondrial genome of Manis pentadactyla pentadactyla (Mammalia: Pholidota), an endemic subspecies of Chinese pangolin: mitogenome characterisation and phylogenetic implications

Comparison of Venous Blood Gas and Biochemical Parameters in Sunda Pangolin (Manis javanica) and Chinese Pangolin (Manis pentadactyla) before and after Isoflurane Anesthesia

Contact Info

Product

Resources

About