“…Based on these characters, we confirmed our meristic data indicating the existence of a new Yuriria genus taxon in the Ameca river basin. Assuming a molecular clock of 1 million years per 1% pairwise differences, which is the generally accepted rate for the cytochrome b gene in other cyprinids, including Notropis species from Central Mexico (Dowling et al, 2002;Shönhunth, 2002;Doadrio & Carmona, 2004), the Yuriria species from the Ameca river diverged from the other species of Yuriria in contiguous basins during the Pliocene, approximately 3.8 Mya. These results are consistent with findings for other pairs of sister species inhabiting the Ameca river basin and other drainages of Central Mexico (e.g., cladogenesis of Allotoca goslinae Miller, 1987 andAmeca splendens Miller &Fitzsimons, 1971, with an estimated divergence time of 3.7 Mya, sensu Doadrio & Domínguez-Domínguez, 2004).…”