Phylogenetic Relationships Inferred from Ribosomal ITS Sequences and Biogeographic Patterns in Representatives of the Genus Calopteryx (Insecta: Odonata) of the West Mediterranean and Adjacent West European Zone

Weekers, Peter H. H.; Jonckheere, Johan F. De; Dumont, Henri J.

doi:10.1006/mpev.2001.0947

Cited by 103 publications

(84 citation statements)

References 22 publications

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“…Such a differentiation is consistent with previous morphological and phylogenetic studies by Dumont (1972), Weekers et al (2001) and Dumont et al (2005a), who conclude that the west Mediterranean refugium allowed xanthostoma to survive the glaciations. Hybridization between the taxa splendens and xantostoma is reported: Papazian (1995) claims there is little contact between them in the south of France, but Pavesi (pers.…”

Section: Discussionsupporting

confidence: 92%

Genetic diversity, population structure and taxonomy of Calopteryx splendens (Odonata: Calopterygidae): An AFLP analysis

Sadeghi¹,

Kyndt²,

Dumont³

2010

Eur. J. Entomol.

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Abstract. Calopteryx splendens is a widely distributed palaearctic damselfly with a remarkably uniform morphology. Variation in the size and shape of the pigmented spot on the wing is the main diagnostic character used to discriminate subspecies across its huge geographic range. Here, AFLP analysis was used to assess the genetic structure and diversity of nine populations representing 3 putative subspecies and evaluate the pigment spot as a taxonomic marker. Genetic diversity was high, with the number of polymorphic loci per population ranging from 141 to 280 out of a total of 333 variable sites (42.3-84.1%) and Nei's gene diversity from 0.160 to 0.283 (overall 0.299). Overall population genetic differentiation (FST = 0.2766) suggests limited gene flow and adaptation to local environments. Restricted gene flow and genetic differentiation among populations are supported by significant FST estimates. High levels of gene flow (Nm >1) were only recorded among three Asian populations (Russia -Kazakhstan -Turkey). The patterns of genotypic diversity suggest that a given wing spot size and shape may arise from the hybridization of a limited number, possibly not more than four, ancestral gene pools in different ways and at different times. Clearly, the sample analyzed was not sufficient to capture all of the complex history of C. splendens, but sufficient to indicate the taxa ancilla, waterstoni, and orientalis possibly represent three of the four ancestral gene pools, and originated in western Asia. The origin of the fourth, xanthostoma, is the western Mediterranean.

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Section: Discussionsupporting

confidence: 92%

Genetic diversity, population structure and taxonomy of Calopteryx splendens (Odonata: Calopterygidae): An AFLP analysis

Sadeghi¹,

Kyndt²,

Dumont³

2010

Eur. J. Entomol.

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“…The ribosomal internal transcriber spacer regions (ITS 1 and 2) are noncoding and therefore evolve rapidly, usually showing concerted evolution (Dover, 1982). The length of the ITS regions in insects is usually short, ranging from 190 -633 bp (Honda et al, 1998;Kuperus and Chapco, 1994;Gómez-Zurita et al, 2000;Weekers et al, 2001), although sizes from 1700 to 600 bp has been also observed (Gallego and Galiµn, 2001). Within the Apidae these regions show an extreme length variation: in subspecies of Apis mellifera the ITS1 is 132 bp (De la Rffla et al, 2007) and in Bombus lapidarius is 289 bp (Ji et al, 2003) whereas it varies from 1387 -1417 bp in species of Melipona (Fernandes-Salom¼o et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Sequence and RFLP analysis of the ITS2 ribosomal DNA in two Neotropical social bees, Melipona beecheii and Melipona yucatanica (Apidae, Meliponini)

et al. 2007

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Two stingless bees species of the genus Melipona, M. beecheii and M. yucatanica, are the only ones reported for the Yucatan Peninsula. The natural distribution of M. beecheii ranges from southern Mexico to Costa Rica, that of M. yucatanica from south Mexico to Guatemala. Colonies of both species occur in a variety of habitats and show adaptations to local conditions denoting the occurrence of ecotypes. The ITS2 of ribosomal DNA has been characterized in both species and its utility to discriminate among colonies has been investigated through RFLP experiments. The ITS2 region is unusually long, 1788 bp in M. beecheii and 1845 bp in M. yucatanica (including the 3' end of the 5.8S gene and partial 5' of the 28S gene). Mean nucleotide divergence between both ITS2 sequences is 16 % (excluding sites with insertions/ deletions) and 20 % when the insertions/deletions are taken into account. The G+C content in both sequences is close to 53 %. The PCR-RFLP assay was performed with 12 restriction enzymes on colonies of M. beecheii from Mexico (Yucatan, Campeche and Chiapas) Costa Rica, El Salvador and Guatemala, and of M. yucatanica from Mexico (Yucatan) and Guatemala. The restriction patterns obtained allow to discriminating colonies of both species with different origins. Both kinds of data are thus useful for assessing intra and interspecific genetic variability and for developing appropriate conservation strategies for these species.

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“…On the other hand, comparisons among ribosomal DNA internal transcribed spacer (rDNA ITS) sequences have been helpful to ascertain evolutionary relatedness of closely related species in diverse insect groups, owing to their high evolutionary rates (Vogler and DeSalle, 1994;Schlötterer et al, 1994: Di Muccio et al, 2000Weekers et al, 2001, Mukha et al, 2002. Recently, studies of Coleoptera and Hemiptera demonstrated the usefulness of ITS-1 and ITS-2 markers in discovering phylogenetic relationships at higher levels such as tribe and subfamily (Marcilla et al, 2001;Von der Schulenburg, 2001).…”

Section: Introductionmentioning

confidence: 99%

The first internal transcribed spacer (ITS-1) of Melipona species (Hymenoptera, Apidae, Meliponini): characterization and phylogenetic analysis

et al. 2005

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Internal transcribed spacer 1 (ITS-1) sequences of the nuclear rDNA of eight bee species of the genus Melipona were studied. Complete ITS-1 sequence and flanking regions from three Melipona species were PCR-amplified, cloned, sequenced, and their variability compared. These sequences show length variation (1391 to 1417 bp), several repeated elements of one, two, three, and four nucleotides, and a repeated tandem sequence of approximately 80 bp. The low variation level between M. quadrifasciata and M. mandacaia sequences supports the hypothesis that they diverged recently. PCR-amplification, cloning, and sequencing of a partial ITS-1 sequence (394 to 496 bp) of eight Melipona species and two outgroups were performed and the obtained sequences used for phylogenetic analysis. The single tree estimated from parsimony analysis recovered four well-defined clades and monophyly of the genus Melipona. The phylogenetic relationships derived from sequences of ITS-1 fragments corroborate the taxonomic classification of Melipona based on morphological characters.

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Phylogenetic Relationships Inferred from Ribosomal ITS Sequences and Biogeographic Patterns in Representatives of the Genus Calopteryx (Insecta: Odonata) of the West Mediterranean and Adjacent West European Zone

Cited by 103 publications

References 22 publications

Genetic diversity, population structure and taxonomy of Calopteryx splendens (Odonata: Calopterygidae): An AFLP analysis

Genetic diversity, population structure and taxonomy of Calopteryx splendens (Odonata: Calopterygidae): An AFLP analysis

Sequence and RFLP analysis of the ITS2 ribosomal DNA in two Neotropical social bees, Melipona beecheii and Melipona yucatanica (Apidae, Meliponini)

The first internal transcribed spacer (ITS-1) of Melipona species (Hymenoptera, Apidae, Meliponini): characterization and phylogenetic analysis

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