Phylogenetic relationships of Cajanus cajan (L.) Millsp. (pigeonpea) and its wild relatives based on seed protein profiles

Abstract

Total seed storage protein of 9 accessions of cultivated C. cajan and 10 wild Cajanus species was reported and compared by sodium dodecyl sulphate polyacrylamide gel electrophoresis. A considerable variation was detected among the protein profiles of different accessions of C. cajan while those of wild species were very specific and distinctly different from each other. Relative similarities between various taxa were estimated by Jaccard's similarity index and cluster analysis was performed to produce a UPGMA dendrogram. The clustering of 10 wild species and C. cajan more or less agrees with their sectional classification and available data based on morphological characteristics, crossability, genome pairing in hybrids and nuclear RFLPs. The species closest to C. cajan is C. cajanifolia although the accessions of C. ca jan also share some bands present in the profiles of C. scarabaeoides, C. goensis, C. lineatus, C. acutifolius and C. volubilis. This points towards polyphyletic origin of the cultigen which has been discussed in this paper.

Evaluation of Genetic Diversity in Vigna radiata (L.) Using Protein Profiling and Molecular Marker (RFLP)

“…The SDS-PAGE have provided a promising tool to distinguish cultivars of a particular crop species (Jha and Ohri, 1996). It has been used successfully to resolve taxonomic and evolutionary problems of several plants (Rabbani et al, 2001).…”

Genetic Diversity and Allele Mining in Soybean Germplasm

“…They include protein techniques and isozymes. The protein techniques are practical and reliable methods for cultivars and species identification because seed storage proteins are largely independent of environmental fluctuation (Sammour, 1992(Sammour, , 1999Camps et al, 1994;Jha and Ohri, 1996). They are less expensive as compared to DNA markers.…”

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“…Since protein composition is genetically determined, it is not subjected to environmental conditions as well as the location of the genes coding for particular proteins in several cases (Aiken andGardiner 1991, Cai andBulletin 1992). Electrophoretic analyses of proteins have advantages over traditional identification techniques in that it offers a rapid and reliable evaluation of genetic differences among most cultivars (Ferguson and Grabe 1986, Gardiner and Fered 1987, 1988, it is a promising tool to distinguish cultivars of a particular crop species (Illoh 1990, Moller and Spoor 1993, Jha and Ohri 1996, Essiet and Illoh 1997, Folorunso and Olorode, 2002. The choice of protein as an index of biomarker in this study is because it is easier to establish cultivar relationships and involves simple technology that can easily be adapted in countries like Nigeria.…”