Phylogenetic relationships of European strains of porcine reproductive and respiratory syndrome virus (PRRSV) inferred from DNA sequences of putative ORF-5 and ORF-7 genes

Suárez, Paula; Zardoya, Rafael; Mj, Martin; Prieto, C.; Dopazo, Joaquı́n; Solana, A.; Jm, Castro

doi:10.1016/0168-1702(95)01305-9

Cited by 97 publications

(68 citation statements)

References 17 publications

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“…Previous studies have found very low levels of sequence variability in European-type PRRSV from Great Britain, Belgium, France, Germany, The Netherlands, and Spain (17,19,30,69). Forsberg et al (19) confirmed the scant isolate variation in these countries but found diversity to be high among type 1 PRRSV when isolates from Italy and Denmark were included in the analysis.…”

Section: Discussionsupporting

confidence: 52%

“…For evolutionary studies, the nucleotide sequences corresponding to ORF 3, 5, or 7 were aligned to previously published database sequences (10,17,18,19,30,36,46,69) by using CLUSTAL X (gap opening penalty, 15; gap extension penalty, 6.66) (71). Following alignment, the phylogeny of the isolates was estimated by using the TREE-PUZZLE program and yielded the phylogenetic trees shown in Fig.…”

Section: Amino Acid Alterations In Europrrsv Nsp1␤ and Nsp2mentioning

confidence: 99%

“…Several members of the nidovirus order have been shown to undergo high-frequency recombination (50,81,83). Because of unique recombinatorial properties (81,83), widespread persistence, intermingling of virus types, and changes in swine management, PRRSV has been a key subject of evolutionary interest (17,18,19,33,39,67,69).…”

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confidence: 99%

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Characterization of Emerging European-Like Porcine Reproductive and Respiratory Syndrome Virus Isolates in the United States

Ropp

Wees

Fāng

et al. 2004

J Virol

165

124

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European-like field isolates of porcine reproductive and respiratory syndrome virus (PRRSV) have recently emerged in North America. The full-length genomic sequence of an index isolate characterized in 1999, strain EuroPRRSV, served as the reference strain for further studies of the evolution and epidemiology of Europeanlike isolates (type 1) in the United States. Strain EuroPRRSV shared 90.1 to 100% amino acid identity with the prototype European strain, Lelystad, within the structural and nonstructural open reading frames (ORFs) and 95.3% overall nucleotide identity. The 5 untranslated region and two nonstructural regions within ORF 1 were closely examined due to significant divergence from strain Lelystad. A 51-bp deletion in a region within ORF 1a, coding for nonstructural protein 2 (NSP2), was observed. Sequence analysis of the structural ORFs 2 to 7 of additional European-like isolates indicated that these isolates share 93% nucleotide identity with one another and 95 to 96% identity with the Lelystad strain but only 70% identity with the North American reference strain VR-2332. Phylogenetic analysis with published PRRSV ORF 3, 5, and 7 nucleotide sequences indicated that these newly emerging isolates form a clade with the Lelystad and United Kingdom PRRSV isolates. Detailed analysis of four of these isolates with a panel of 60 monoclonal antibodies directed against the structural proteins confirmed a recognition pattern that was more consistent with strain Lelystad than with other North American isolates.

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Section: Discussionsupporting

confidence: 52%

Section: Amino Acid Alterations In Europrrsv Nsp1␤ and Nsp2mentioning

confidence: 99%

See 1 more Smart Citation

Characterization of Emerging European-Like Porcine Reproductive and Respiratory Syndrome Virus Isolates in the United States

Ropp

Wees

Fāng

et al. 2004

J Virol

165

124

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“…Based on comparison of nucleotide sequences of these different strains, the European and North American strains were classified into two different genotypes. These two genotypes exhibited only 55% to 80% nucleotide identity (Meng et al, 1995;Murtaugh et al, 1995;Suarez et al, 1996;Meulenberg et al, 1997). High level of genetic variability has also been described within the American group of PRRSV isolates (Andreyev et al, 1997).…”

mentioning

confidence: 99%

“…RT-PCR was carried out using Titan TM One Tube kit (Roche). Two 20-base oligonucleotides were used as primers in RT-PCR reaction designed from a part of ORF6 and the whole ORF7 genes (Suarez et al, 1996). Forward and reverse primers were synthesized as follows: 5'-GTACATTCTGGCCCCTGCCC-3' and 5'-TCGCCCTAATTGAATAGGTG-3'.…”

Section: Reverse Transcription -Polymerase Chain Reaction (Rt-pcr)mentioning

confidence: 99%

Restriction fragment length polymorphism of ORF6 and ORF7 genes of porcine reproductive and respiratory syndrome virus (PRRSV) vaccine strains registered in the Czech Republic

Kosinova¹,

Pšikal²

2006

Vet. Med. - Czech

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ABSTRACT:Restriction fragment length polymorphism (RFLP) of open reading frames 6 and 7 was applied to comparative genetic analysis of live attenuated vaccine strains (Amervac-PRRS/A3, Porcilis PRRS, Ingelvac PRRS) of porcine reproductive and respiratory syndrome virus (PRRSV), registered in the Czech Republic, six field viruses (L-588, L-1606, L-2053, L-3305, L-6558, L-6791), and three PRRSV local field isolates (CAMP V-502, CAMP V-503, VOS 2878) found in pig herds in the Czech Republic and Slovak Republic. The set of restriction enzymes Hae II, Alu I and BsaJ I allowed the differentiation of local field isolates, field viruses of PRRS, and vaccine strains of the European genotype from North American genotype, but could also distinguish between viruses of the same genotype. Five different RFLP patterns were obtained from twelve examined PRRS viruses by combination of the above restriction enzymes. RFLP code 1-1-1 was the most frequent digestion pattern within all PRRS field viruses (L-588, L-1606, L-2053, L-3305, L-6558, L-6791), CAMP V-502 isolate and vaccine strain Porcilis PRRS, which is suggestive of higher antigenic identity among the compared viruses. In the North American types (Ingelvac PRRS vaccine strain and VOS 2878 isolate), homogeneity in restriction patterns (code 2-x-4) was recorded. These studies indicate that PCR-based RFLP analysis of ORF6 and ORF7 of genes might be a suitable tool in epidemiological studies of PRRSV, similarly to the studies based on genetic analysis of ORF5 gene.

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Yansuanmalingua inhibits replication of type 2 porcine reproductive and respiratory syndrome virus via activating the caspase‐8 apoptosis pathway

Ding

Cheng³

et al. 2020

J Basic Microbiol

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Porcine reproductive and respiratory syndrome virus (PRRSV) causes significant economic losses to the pork industry. The present study showed that Yansuanmalingua (YASML) can inhibit type 2 PRRSV replication using plaque assay, quantitative reverse transcriptase-polymerase chain reaction, and immunofluorescence assay. Furthermore, inhibition of PRRSV replication was shown to be related to Toll-like receptor 3 (TLR3)-dependent apoptosisinduction by YASML in the PRRSV-infected MARC-145, and TLR3-dependent apoptosis-induction by YASML was found to suppress PRRSV replication via the activation of caspase-8 and caspase-3 pathways, respectively. Meanwhile, activation of the caspase-3 pathway seemed to be related to the downregulation of myeloid cell leukemia 1 (Mcl-1) expression. Our results showed that YASML-induced TLR3-dependent apoptosis could be blocked by a pan-caspase inhibitor and small interfering RNA against TLR3. In conclusion, the present study demonstrates that YASML exerts its anti-PRRSV effect by activating the caspase-8/caspase-3 signaling pathway and by negatively regulating Mcl-1 expression. These findings not only provide new insights into the molecular mechanism of YASML inhibition of PRRSV replication via the TLR3-dependent apoptosis pathway but also suggest potential, new antiviral drugs by expressing caspase-3 or down expressing Mcl-1. K E Y W O R D Sapoptosis, caspase-3, caspase-8, siRNA, Toll-like receptor 3, Yansuanmalingua

show abstract

Phylogenetic relationships of European strains of porcine reproductive and respiratory syndrome virus (PRRSV) inferred from DNA sequences of putative ORF-5 and ORF-7 genes

Cited by 97 publications

References 17 publications

Characterization of Emerging European-Like Porcine Reproductive and Respiratory Syndrome Virus Isolates in the United States

Characterization of Emerging European-Like Porcine Reproductive and Respiratory Syndrome Virus Isolates in the United States

Restriction fragment length polymorphism of ORF6 and ORF7 genes of porcine reproductive and respiratory syndrome virus (PRRSV) vaccine strains registered in the Czech Republic

Yansuanmalingua inhibits replication of type 2 porcine reproductive and respiratory syndrome virus via activating the caspase‐8 apoptosis pathway

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