Phylogenetical Relationships between Kejobong Goat and Other Goats Based on Mt-DNA D-loop Sequence Analysis

Lestari, Dewi Puji; Purbowati, Endang; Sutopo, S.; Kurnianto, Edy

doi:10.5398/tasj.2018.41.2.85

Cited by 11 publications

(12 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A total of 45 Simeulue buffaloes collected from three subdistricts for measurement their body size characteristics. The buffalo samples were grouped into two age categories, 4-6 and 2-4 years with criteria according to Lestari et al (2018). Blood sampling was carried out by collecting about 3 mL of blood samples from buffalo through the jugular vein using a venoject and vacutainer tubes with EDTA and then stored at 4⁰C.…”

Section: Field Sampling and Blood Sample Collectionmentioning

confidence: 99%

Body size characteristics and polymorphism in GH and GHRH genes of Simeulue Buffalo of Aceh, Indonesia

Eriani

Rahmi

Jamil³

et al. 2018

Biodiversitas

View full text Add to dashboard Cite

Eriani K, Rahmi R, Jamil I, Rosnizar R, Azhar A. 2019. Body size characteristics and polymorphism in GH and GHRH genes of Simeulue Buffalo of Aceh, Indonesia. Biodiversitas 20: 236-242. This study was done to determine body size characteristics, to identify the presence of polymorphism in the GH/MspI and GHRH/HaeIII genes, and to analyze the relationship between GH and GHRH gene polymorphisms to body size characteristics in Simeulue buffalo. Experimental animals used were 45 Simeulue buffalo randomly sampled from three subdistricts, i.e., Salang, Alafan and Simeulue Cut, fifteen from each subdistrict. Body size characteristics measured were withered height, body length, chest circumference, chest depth, and hip height. A 5 mL whole blood was collected from fifteen out of forty five by using EDTA as an anticoagulant. Extraction of genomic DNA and analysis of GH/MspI and GHRH/HaeIII polymorphisms was done using GeneAid DNA extraction kit and polymerase chain reaction-restriction length fragment polymorphism (PCR-RFLP) method, respectively. The data of body size characteristics which includes mean value, standard deviation, and coefficient of variation were analyzed descriptively. The molecular data represented by DNA banding pattern was used to calculate allele and genotype frequencies, expected (He) and observed (Ho) heterozygosities, and Hardy-Weinberg equilibrium. The relationship between genotype and body size characteristic was analyzed using t-test. The results showed that coefficient of variation in body size characteristics was low, ranging from 5.39-16.06% in the age category of 4-6 years old and ranging from 5.25-11.27% in the age category of 2- 4 years old. The results of the molecular analysis indicated that GH/MspI locus was monomorphic by the presence of one (+/+) genotype. GHRH/HaeIII locus was polymorphic as shown by the presence of 3 genotypes namely AA (0.13), AB (0.80) and BB (0.07). Expected heterozygosity (0.80) was higher than observed heterozygosity (0.49). The frequency of GHRH/HaeIII agreed with Hardy Weinberg equilibrium. GHRH/HaeIII polymorphism did not significantly influence (P>0.05) body size characteristics of Simeulue buffalo.

show abstract

Section: Field Sampling and Blood Sample Collectionmentioning

confidence: 99%

Body size characteristics and polymorphism in GH and GHRH genes of Simeulue Buffalo of Aceh, Indonesia

Eriani

Rahmi

Jamil³

et al. 2018

Biodiversitas

View full text Add to dashboard Cite

show abstract

“…This similarity of amino acid mutation position perhaps related to the assumption that Kejobong goat was a crossbred of Kacang goat and Etawah Grade goat [ 2 , 3 ], and it was confirmed by Lestari et al . [ 42 ] reporting that by phylogenetic relationship analysis based on D-loop sequence, Kejobong goat was in the same cluster together with Kacang goat and Etawah Grade goat with no clear grouping in each breed in B haplogroup. Meanwhile, as comparator based on other mtDNA genes, Kejobong goat had 11 nucleotide polymorphisms on the basis of 548 bp D-loop sequence that formed 7 haplotypes [ 8 ].…”

Section: Discussionmentioning

confidence: 99%

“…Another study conducted by Lestari et al . [ 42 ], Kejobong goat had 12 nucleotide polymorphisms of 1191 bp D-loop sequence that formed 11 haplotypes with Pi 0.00143±0.00018.…”

Section: Discussionmentioning

confidence: 99%

Amino acid sequence based on Cytochrome b gene in Kejobong goat and its genetic relationships among several local goats in Asia

et al. 2018

View full text Add to dashboard Cite

Aim:This study aimed to analyze the amino acid sequence of Cytochrome b (Cyt b) gene in Kejobong goat and its genetic relationships with local goats located in Asia.Materials and Methods:A total of 28 heads of Kejobong goat were purposively sampled. The deoxyribonucleic acid (DNA) was extracted from blood using gSYNC DNA mini kit (Geneaid Biotech Ltd.). Cyt b gene was amplified using polymerase chain reaction (PCR) method with CytbCapF and CytbCapR primers. The amplified PCR products were sequenced for further analysis.Results:There were a total 377 amino acid sequences translated from 1140 base pair (bp) of Cyt b gene, 99.20% of it were monomorphic, amino acid alterations were found at site 16th, 121st, and 231st, and Kejobong goat was in the same cluster with Southeast Asian local goats.Conclusion:Most of the amino acid sequence on Cyt b gene in Kejobong goat is monomorphic (99.20%), only a few nucleotide mutations were found that causing amino acid alteration in three sites (0.80%). Kejobong goat has a close genetic relationship to several local goats in Southeast Asian.

show abstract

“…The Kejobong goat is known as an indigenous Indonesian breed raised by a semi-intensive animal farming system by local farmers. This goat has been confirmed to be the progeny of a cross between Kacang and Etawah grade goats [1,2]. The Kejobong goat is known not only for its prolific traits but also for its high rate of growth, good carcass composition, and good reproductive performance [3].…”

Section: Introductionmentioning

confidence: 99%

Effect of insulin-like growth factor 1 gene on growth traits of Kejobong goat and its growth analysis

et al. 2020

View full text Add to dashboard Cite

Aim: This study aimed to identify the effect of the insulin-like growth factor 1 (IGF1) gene on growth, to uncover the genetic marker at the IGF1 gene, and to predict growth performance by analyzing growth models of Kejobong goats based on their genotype. Materials and Methods: DNA and records of body weight (BW) and body measurements (BM) of Kejobong goats were collected, the IGF1 gene was amplified from the DNA template by polymerase chain reaction (PCR); the PCR products were then sequenced to determine single nucleotide polymorphisms (SNP). Linear mixed model (LMM) was used to analyze the association between SNP and growth traits. Four non-linear growth models were analyzed using non-LMM to describe the growth model and to compare the growth within genotypes. Results: An SNP at intron 4 (g5752G→C) genotyped into GG and CC was significantly associated with BW and BM. Goats of genotype GG had a significantly higher BW and BM (p<0.05) than those of genotype CC. Growth analysis showed that the von Bertalanffy model was the most fit for describing BW, the Brody model for chest width and hip height, the Gompertz and Logistic models for heart girth, and the von Bertalanffy and Gompertz models for hip width. Conclusion: An SNP at intron 4 of the IGF1 gene was associated with the growth trait and was usable as a genetic marker candidate for improvement of growth traits of Kejobong goats while von Bertalanffy model provides proper and accurate estimates of parameters to describe the growth performance of Kejobong goats.

show abstract

Phylogenetical Relationships between Kejobong Goat and Other Goats Based on Mt-DNA D-loop Sequence Analysis

Cited by 11 publications

References 52 publications

Body size characteristics and polymorphism in GH and GHRH genes of Simeulue Buffalo of Aceh, Indonesia

Body size characteristics and polymorphism in GH and GHRH genes of Simeulue Buffalo of Aceh, Indonesia

Amino acid sequence based on Cytochrome b gene in Kejobong goat and its genetic relationships among several local goats in Asia

Effect of insulin-like growth factor 1 gene on growth traits of Kejobong goat and its growth analysis

Contact Info

Product

Resources

About