Phylogeny and divergence times inferred from rps16 sequence data analyses for Tricyrtis (Liliaceae), an endemic genus of north-east Asia

Abstract

The rate of molecular evolution of Tricyrtis plant groups as a model system was estimated. The outcome further highlights the importance of conserving biodiversity in a rapidly changing Earth environment. This phylogenetic analyses of Tricyrtis with its high endemism in north-east Asia sheds light on processes of speciation processes.

Phylogeny and Molecular Evolution of Tricyrtis (Liliaceae s.l.) Inferred from Plastid DNA matK Spacer Nucleotide Sequences

“…To amplify matK spacers, two primers were used, 3914F (5´-TGG GTT GCT AAC TCA ATG G-3´) and 122SR (TGA AAG AGA AGC GGG TA). For the amplifications of rps16 introns (Hong & Jury, 2011), the primers were rpsF (GTG GTA GAA AGC AAC GTG CGA CTT) and rpsR2 (TCG GGA TCG AAC ATC AAT TGC AAC). For automatic sequencing reactions, a 373 DNA sequencer (Applied Biosystems) was used.…”

“…The common name of the genus is "toad lily". Although there is no fossil record of a Tricyrtis ancestor, the high endemism in Asia and their localized distribution patterns attracts attention in terms of evolutionary routes and diversification relationships among the taxa (Hong & Jury, 2011). In the current study we compare phylogenetic relationships among Tricyrtis groups based on analysis of matK spacer and rps16 intron nucleotide sequences and discuss the differences in evolutionary rates and molecular dating found.…”

“…The only available phylogenetic information on the genus Tricyrtis (Liliaceae s.l.) comes from our recent Bayesian inference studies (Hong & Jury, 2011). In the current work, classical parsimony analyses on Tricyrtis have been carried out to establish phylogenetic relationships for further molecular evolutionary studies using newly sequenced matK spacer and relatively recent publication of rps16 intron nucleotide sequence data by the same authors (Hong & Jury, 2011).…”

“…comes from our recent Bayesian inference studies (Hong & Jury, 2011). In the current work, classical parsimony analyses on Tricyrtis have been carried out to establish phylogenetic relationships for further molecular evolutionary studies using newly sequenced matK spacer and relatively recent publication of rps16 intron nucleotide sequence data by the same authors (Hong & Jury, 2011). JQ957544, JN561092 JN561093 JQ957545, JN561094 JQ957546, JN561095 JQ957547, JN561096 JQ957548, JN561097 JQ957549 JQ957550 JQ957551 JQ957552, JN561099 JQ957553 JQ957554, JN561100 JQ957557 JQ957560, JN561105 JQ957558, JN561103 JQ957559, JN561104 JN561098 JN561101 JQ957561, JN561106 JQ957562, JN561107 JQ957563 JQ957564 JQ957565 * For type specimen information on Tricyrtis, see Hong and Jury (2011).…”

“…The matK spacer sequencing data was obtained with rps16 intron data during the same period of works at the University of Reading (UK) and Royal Botanic Gardens, Kew. Total DNA was extracted from fresh leaves of 19 taxa (Tricyrtis) ( Table 1; Hong & Jury, 2011). Mature leaves from eight taxa were collected directly from Kew (K).…”