Phylogeny of Canadian ergot fungi and a detection assay by real-time polymerase chain reaction

Shoukouhi, Parivash; Hicks, Carmen; Menzies, J. I.; Popovic, Zlatko; Chen, Wen; Seifert, Keith A.; Assabgui, R.; Liu, Miao

doi:10.1080/00275514.2019.1581018

Cited by 15 publications

(21 citation statements)

References 36 publications

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“…Within C. sect. Claviceps we observed support for the separation of recently described species from C. purpurea s.s. (Fig.1) (Pažoutová et al 2000, 2002, 2015; Douhan et al 2008; van der Linde et al 2016; Shoukouhi et al 2019; Liu, M et al Submitted ). Overall, C. sect.…”

Section: Resultsmentioning

confidence: 98%

“…Similar disparate lineage sorting was observed in the classification of recent cryptic species in C. sect. Claviceps (Shoukouhi et al 2019; Liu, M et al Submitted ), suggesting similar evolutionary patterns could be driving the diversification of C. sect. Claviceps .…”

Section: Resultsmentioning

confidence: 99%

“…Multigene studies have confirmed this in C. purpurea s.l. with evidence pointing towards adaptation to ecological niches, and division into 8 different lineages (Pažoutová et al 2000, 2002, 2015; Douhan et al 2008; Shoukouhi et al 2019; Liu, M et al Submitted ). However, these cryptic species contain overlapping host ranges, geographic distributions, and alkaloid production.…”

Section: Introductionmentioning

confidence: 99%

“…However, these cryptic species contain overlapping host ranges, geographic distributions, and alkaloid production. In addition, individual gene phylogenies showed cases of disparate results (Shoukouhi et al 2019; Liu, M et al Submitted ) and estimates of genetic differences between cryptic species show relatively low average number of nucleotide substitutions per site ranging from 0.006 to 0.025 (Pažoutová et al 2015).…”

Section: Introductionmentioning

confidence: 99%

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Whole genome comparisons of ergot fungi reveals the divergence and evolution of species within the genus Claviceps are the result of varying mechanisms driving genome evolution and host range expansion

Wyka

Mondo

Liu

et al. 2020

Preprint

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1 2 Genus-wide comparison reveals divergence and evolution of the four sections within the 3 genus Claviceps are the result of varying mechanisms driving genome evolution and host 4 range 5 6 Abstract: 16 Background: 17 The genus Claviceps has been known for centuries as an economically important fungal 18 genera for pharmacology and agricultural research. Despite this little is known about its 19 evolution. Only recently have researchers begun to unravel the evolutionary history of the genus, 20 with origins in South America and classification of four distinct sections through ecological, 21 morphological, and metabolic features (Claviceps sects. Citrinae, Paspalorum, Pusillae, and 22 Claviceps). The first three sections are additionally characterized by narrow host range, while 23 sect. Claviceps is considered evolutionarily more successful and adaptable as it has the largest 24 host range and biogeographical distribution. However, the reasons for this success and 25 adaptability remain unclear.26Results: 27 Our study elucidates these factors by sequencing and annotating 50 Claviceps genomes, 28 representing 21 species, for a comprehensive comparison of genome architecture and plasticity 29 in relation to host range potential. Our results show the trajectory from specialized one-speed 30 genomes (C. sects. Citrinae and Paspalorum) to adaptive two-speed genomes (C. sects. Pusillae 31 and Claviceps) through co-localization of TEs around predicted effectors and loss of RIP 32 resulting in unconstrained tandem gene duplication coinciding with increased host range 33 potential and speciation.34 Conclusions: 35 Alterations of genomic architecture and plasticity can substantially influence and shape 36 the evolutionary trajectory of fungal pathogens and their adaptability. Furthermore, our study 37 provides a large increase in available genomic resources to propel future studies of Claviceps in 38 pg. 3 pharmacology and agricultural research, as well as, research into deeper understanding of the 39 evolution of adaptable plant pathogens. 40 41

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Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Whole genome comparisons of ergot fungi reveals the divergence and evolution of species within the genus Claviceps are the result of varying mechanisms driving genome evolution and host range expansion

Wyka

Mondo

Liu

et al. 2020

Preprint

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“…While common land grasses are often infected by both C. purpura sensu stricto ( s. s. ) and C. humidiphila , cereal crops seem to be infected by just C. purpurea s. s. In the Palearctic region, these two species are the most important from an agricultural point of view [2,3]. Furthermore, recent findings suggest further cryptic diversification among North American C. purpurea s. l. specimens and their impacts on agricultural remains uncertain [4].…”

Section: Introductionmentioning

confidence: 99%

Ergochromes: Heretofore Neglected Side of Ergot Toxicity

Flieger

Stodůlková

Wyka

et al. 2019

Toxins

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Ergot, fungal genus Claviceps, are worldwide distributed grass pathogens known for their production of toxic ergot alkaloids (EAs) and the great agricultural impact they have on both cereal crop and farm animal production. EAs are traditionally considered as the only factor responsible for ergot toxicity. Using broad sampling covering 13 ergot species infecting wild or agricultural grasses (including cereals) across Europe, USA, New Zealand, and South Africa we showed that the content of ergochrome pigments were comparable to the content of EAs in sclerotia. While secalonic acids A–C (SAs), the main ergot ergochromes (ECs), are well known toxins, our study is the first to address the question about their contribution to overall ergot toxicity. Based on our and published data, the importance of SAs in acute intoxication seems to be negligible, but the effect of chronic exposure needs to be evaluated. Nevertheless, they have biological activities at doses corresponding to quantities found in natural conditions. Our study highlights the need for a re-evaluation of ergot toxicity mechanisms and further studies of SAs’ impact on livestock production and food safety.

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Sympatric divergence of the ergot fungus, Claviceps purpurea, populations infecting agricultural and nonagricultural grasses in North America

Liu

Shoukouhi

Bisson

et al. 2020

Ecology and Evolution

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Phylogeny of Canadian ergot fungi and a detection assay by real-time polymerase chain reaction

Cited by 15 publications

References 36 publications

Whole genome comparisons of ergot fungi reveals the divergence and evolution of species within the genus Claviceps are the result of varying mechanisms driving genome evolution and host range expansion

Whole genome comparisons of ergot fungi reveals the divergence and evolution of species within the genus Claviceps are the result of varying mechanisms driving genome evolution and host range expansion

Ergochromes: Heretofore Neglected Side of Ergot Toxicity

Sympatric divergence of the ergot fungus, Claviceps purpurea, populations infecting agricultural and nonagricultural grasses in North America

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