Phylogeny of dengue virus type 3 circulating in Colombia between 2001 and 2007

Dengue, the predominant arthropod-borne viral disease affecting humans, is caused by one of four distinct serotypes (DENV-1, -2, -3 or -4). A literature analysis and review was undertaken to describe the molecular epidemiological trends in dengue disease and the knowledge generated in specific molecular topics in Latin America, including the Caribbean islands, from 2000 to 2013 in the context of regional trends in order to identify gaps in molecular epidemiological knowledge and future research needs. Searches of literature published between 1 January 2000 and 30 November 2013 were conducted using specific search strategies for each electronic database that was reviewed. A total of 396 relevant citations were identified, 57 of which fulfilled the inclusion criteria. All four dengue virus serotypes were present and co-circulated in many countries over the review period (with the predominance of individual serotypes varying by country and year). The number of countries in which more than one serotype circulated steadily increased during the period under review. Molecular epidemiology data were found for Argentina, Bolivia, Brazil, the Caribbean region, Colombia, Ecuador, Mexico and Central America, Paraguay, Peru and Venezuela. Distinct lineages with different dynamics were found in each country, with co-existence, extinction and replacement of lineages occurring over the review period. Despite some gaps in the literature limiting the possibility for comparison, our review has described the molecular epidemiological trends of dengue infection. However, several gaps in molecular epidemiological information across Latin America and the Caribbean were identified that provide avenues for future research; in particular, sequence determination of the dengue virus genome is important for more precise phylogenetic classification and correlation with clinical outcome and disease severity.

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“…Colombian isolates clustered apart from Brazilian isolates, which were associated with a significant number of DHF cases and fatalities [61]. …”

Section: Resultsmentioning

confidence: 99%

Dengue in Latin America: Systematic Review of Molecular Epidemiological Trends

et al. 2017

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“…In another study conducted in North-east Colombia, near the Venezuelan border, secondary DENV-2 infections were mainly associated with SDD, but some SDD also resulted from primary DENV-2 infections, while DENV-3 induced SDD (DHF) occurred more frequently during primary DENV infections [26]. During 2001-2007, only strains of the DENV-3 IIIb genotype, were isolated in Colombia, and were different from those present in Brazil or Central American countries [35]. In another study, however, DENV-3 genotype I was identified in several Colombian states, while in three others DENV-3 genotype I and IIIb strains co-circulated, but the pathogenic capacities (SDD-associations) of these DENV-3 genotype I strains were not provided [19].…”

Section: Discussionmentioning

confidence: 99%

Simple Prognostic Criteria Can Definitively Identify Patients Who Develop Severe Versus Non-Severe Dengue Disease, or Have Other Febrile Illnesses

Falconar

Romero-Vivas

2012

J Clin Med Res

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BackgroundSevere dengue disease (SDD) (DHF/DSS: dengue hemorrhagic fever/dengue shock syndrome) results from either primary or secondary dengue virus (DENV) infections, which occur 4 - 6 days after the onset of fever. As yet, there are no definitive clinical or hematological criteria that can specifically identify SDD patients during the early acute febrile-phase of disease (day 0 - 3: < 72 hours). This study was performed during a SDD (DHF/DSS) epidemic to: 1) identify the DENV serotypes that caused SDD during primary or secondary DENV infections; 2) identify simple clinical and hematological criteria that could significantly discriminate between patients who subsequently developed SDD versus non-SDD (N-SDD), or had a non-DENV fever of unknown origin (FUO) during day 0 - 3 of fever; 3) assess whether DENV serotype co-infections resulted in SDD.MethodsFirst serum samples, with clinical and hematological criteria, were collected from 100 patients during the early acute febrile-phase (day 0 - 3: < 72 hours), assessed for DENV or FUO infections by IgM- and IgG-capture ELISAs on paired serum samples and by DENV isolations, and subsequently graded as SDD, N-SDD or FUO patients.ResultsIn this study: 1) Thirty-three patients had DENV infections, predominantly secondary DENV-2 infections, including each SDD (DHF/DSS) case; 2) Secondary DENV-2/-3 and DENV-2/-4 serotype co-infections however resulted in N-SDD; 3) Each patient who subsequently developed SDD, but none of the others, displayed three clinical criteria: abdominal pain, conjunctival injection and veni-puncture bleeding, therefore each of these criteria provided definitively significant prognostic (P < 0.001) values; 4) Petechia, positive tourniquet tests and hepatomegaly, and neutrophilia or leukopenia also significantly identified those who: a) subsequently developed SDD versus N-SDD, or had a FUO; b) subsequently developed SDD versus N-SDD; c) subsequently developed N-SDD versus FUOs, respectively.ConclusionsThis is the first report of simple definitively prognostic criteria for SDD patients, including the first assessment and confirmation of conjunctival injection. The three definitive clinical criteria used alone, or supported by the other four criteria, could be essential for specifically identifying those patients needing prompt hospital-based therapies to lessen or avert SDD, without unnecessary hospitalization of the other patients.KeywordsDengue virus; Severe dengue; Dengue fever; Diagnostic; Criteria; Hemorrhage; Shock

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“…A number of phylogenetically distinct lineages, termed genotypes, have been also identified within each serotype, which may differs in both geographical distribution and viral virulence/transmissibility [3], [4], [5]. Among them, the genotype III of DENV-3 has been frequently associated with severe dengue outbreaks in Asia, Africa and Latin America [6], [7], [8], [9], [10], [11], [12], [13], [14], [15], [16], [17], [18]. DENV-3 genotype III probably emerged in the Indian sub-continent around the middle 1970s and subsequently spread to other countries from Asian, Africa and the Americas [6], [19].…”

Section: Introductionmentioning

confidence: 99%

Origin and Evolution of Dengue Virus Type 3 in Brazil

et al. 2012

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The incidence of dengue fever and dengue hemorrhagic fever in Brazil experienced a significant increase since the emergence of dengue virus type-3 (DENV-3) at the early 2000s. Despite the major public health concerns, there have been very few studies of the molecular epidemiology and time-scale of this DENV lineage in Brazil. In this study, we investigated the origin and dispersion dynamics of DENV-3 genotype III in Brazil by examining a large number (n = 107) of E gene sequences sampled between 2001 and 2009 from diverse Brazilian regions. These Brazilian sequences were combined with 457 DENV-3 genotype III E gene sequences from 29 countries around the world. Our phylogenetic analysis reveals that there have been at least four introductions of the DENV-3 genotype III in Brazil, as signified by the presence of four phylogenetically distinct lineages. Three lineages (BR-I, BR-II, and BR-III) were probably imported from the Lesser Antilles (Caribbean), while the fourth one (BR-IV) was probably introduced from Colombia or Venezuela. While lineages BR-I and BR-II succeeded in getting established and disseminated in Brazil and other countries from the Southern Cone, lineages BR-III and BR-IV were only detected in one single individual each from the North region. The phylogeographic analysis indicates that DENV-3 lineages BR-I and BR-II were most likely introduced into Brazil through the Southeast and North regions around 1999 (95% HPD: 1998–2000) and 2001 (95% HPD: 2000–2002), respectively. These findings show that importation of DENV-3 lineages from the Caribbean islands into Brazil seems to be relatively frequent. Our study further suggests that the North and Southeast Brazilian regions were the most important hubs of introduction and spread of DENV-3 lineages and deserve an intense epidemiological surveillance.

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Phylogeny of dengue virus type 3 circulating in Colombia between 2001 and 2007

Abstract: SummaryDengue

Cited by 12 publications

References 42 publications

Dengue in Latin America: Systematic Review of Molecular Epidemiological Trends

Dengue in Latin America: Systematic Review of Molecular Epidemiological Trends

Simple Prognostic Criteria Can Definitively Identify Patients Who Develop Severe Versus Non-Severe Dengue Disease, or Have Other Febrile Illnesses

Origin and Evolution of Dengue Virus Type 3 in Brazil

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