Phylogeny of symbiotic bacteria of four weevil species (coleoptera: curculionidae) based on analysis of 16S ribosomal DNA

Campbell, Bruce; Bragg, Thomas S.; Turner, Charles E.

doi:10.1016/0965-1748(92)90136-3

Cited by 66 publications

(35 citation statements)

References 38 publications

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“…6). Many other insects, probably all homopterans feeding on plant sap, carry ovum-transmitted cytoplasmic endosymbionts, e.g., leafhoppers (Campbell and Purcell 1993), whiteflies (Clark et al 1992b), as well as coleopteran weevils (Campbell et al 1992). Attempts to find similar plasmids in mycetocyte-bearing insects should reveal the genetic and nutritional basis for these ancient bacterial associations (Buchner 1965).…”

Section: Discussionmentioning

confidence: 99%

Discovery and molecular characterization of a plasmid localized in Buchnera sp. bacterial endosymbiont of the aphid Rhopalosiphum padi

et al. 1995

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We have identified and completely sequenced a novel plasmid isolated from the aphid Rhopalosiphum padi. Evidence which suggests that the plasmid occurs localized within the bacterial endosymbionts is presented. The plasmid contains the four genes that constitute the entire leucine operon. This fact makes it really unique since most plasmids are dispensable and lack genes that encode essential anabolic functions. Four more phloem-feeding aphid species also seem to contain homologous plasmids. Although further work is necessary, we hypothesize that this plasmid has appeared during the evolution of the symbiotic association between the aphid and the bacterial endosymbiont. The fact that this plasmid contains the entire leucine operon can be related to physiological evidence showing that the aphid host's diet of plant phloem is deficient in essential amino acids.

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Section: Discussionmentioning

confidence: 99%

Discovery and molecular characterization of a plasmid localized in Buchnera sp. bacterial endosymbiont of the aphid Rhopalosiphum padi

et al. 1995

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“…albopictus cell culture fluid and from tissues were identical, confirming that the bacterium obtained in culture was the same organism found in the tissues of T. infestans. Comparison of a consensus sequence with 16s rDNA sequences from other selected eubacteria revealed that TI1 was a member of the 7-3 subdivision of the class Proteobacteriu, a group containing many other insect symbionts, including the symbionts of aphids, weevils, whiteflies, and tsetse (1,6,9,11,31,34). The phylogenetic tree (Fig.…”

Section: Resultsmentioning

confidence: 99%

In Vitro Culture and Phylogenetic Analysis of "Candidatus Arsenophonus triatominarum," an Intracellular Bacterium from the Triatomine Bug, Triatoma infestans

Hypša

Dale

1997

International Journal of Systematic Bacteriology

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An intracellular symbiotic bacterium was isolated from the hemolymph of Triatorna infestans and cultured in an Aedes albopictus cell line. 16s ribosomal DNA sequence analysis revealed that the bacterium was a member of the y-3 subgroup of the class Proteobacteria, having 96.2% sequence identity with the most closely related bacterium, Arsenophonus nasoniae, the causative agent of the son-killer trait in the parasitoid wasp Nasonia vitripennis. These bacteria share morphological features and a common tissue distribution and transmission mode. The A. nasoniae-T. infestans symbiont branch represents a lineage of insect symbionts which may be capable of horizontal transmission between phylogenetically distant host insects. We propose that the intracellular symbiont from T. infestans be classified as "Candidatus Arsenophonus triatominarum." The bacterium found in the hemocytes of T. infestans is designated the type strain of this species.

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“…However, we noticed that Campbell et al (1992) published an erroneous sequence of SOPE. This sequence was indeed chimeric (Campbell, personal communication), containing the 5Ј [1-400 nucleotides (nt)] and 3Ј (from 1,100 nt) ends of the Wolbachia rrs gene sequence, the secondary endocytobiote of S. oryzae (Werren et al 1995).…”

Section: Sope Phylogenymentioning

confidence: 99%

Molecular Characterization of the Principal Symbiotic Bacteria of the Weevil Sitophilus oryzae: A Peculiar G + C Content of an Endocytobiotic DNA

et al. 1998

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The principal intracellular symbiotic bacteria of the cereal weevil Sitophilus oryzae were characterized using the sequence of the 16S rDNA gene (rrs gene) and G + C content analysis. Polymerase chain reaction amplification with universal eubacterial primers of the rrs gene showed a single expected sequence of 1,501 bp. Comparison of this sequence with the available database sequences placed the intracellular bacteria of S. oryzae as members of the Enterobacteriaceae family, closely related to the free-living bacteria, Erwinia herbicola and Escherichia coli, and the endocytobiotic bacteria of the tsetse fly and aphids. Moreover, by high-performance liquid chromatography, we measured the genomic G + C content of the S. oryzae principal endocytobiotes (SOPE) as 54%, while the known genomic G + C content of most intracellular bacteria is about 39.5%. Furthermore, based on the third codon position G + C content and the rrs gene G + C content, we demonstrated that most intracellular bacteria except SOPE are A + T biased irrespective of their phylogenetic position. Finally, using the hsp60 gene sequence, the codon usage of SOPE was compared with that of two phylogenetically closely related bacteria: E. coli, a free-living bacterium, and Buchnera aphidicola, the intracellular symbiotic bacteria of aphids. Taken together, these results show a peculiar and distinctly different DNA composition of SOPE with respect to the other obligate intracellular bacteria, and, combined with biological and biochemical data, they elucidate the evolution of symbiosis in S. oryzae.

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Phylogeny of symbiotic bacteria of four weevil species (coleoptera: curculionidae) based on analysis of 16S ribosomal DNA

Cited by 66 publications

References 38 publications

Discovery and molecular characterization of a plasmid localized in Buchnera sp. bacterial endosymbiont of the aphid Rhopalosiphum padi

Discovery and molecular characterization of a plasmid localized in Buchnera sp. bacterial endosymbiont of the aphid Rhopalosiphum padi

In Vitro Culture and Phylogenetic Analysis of "Candidatus Arsenophonus triatominarum," an Intracellular Bacterium from the Triatomine Bug, Triatoma infestans

Molecular Characterization of the Principal Symbiotic Bacteria of the Weevil Sitophilus oryzae: A Peculiar G + C Content of an Endocytobiotic DNA

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