Phylogeny of the Yeast Genera Hanseniaspora (Anamorph Kloeckera), Dekkera (Anamorph Brettanomyces), and Eeniella as Inferred from Partial 26S Ribosomal DNA Nucleotide Sequences

Abstract

Partial 26s ribosomal DNA sequences of species assigned to the genera Hanseniaspora, Klueckera, Dekkera, Brettanomyces, and EenieZZu were determined. A phylogenetic analysis of the sequences showed that the genus EenieZZu is derived within the genus Brettanomyces and that the genus Hanseniaspora (anamorph Kloeckera) is not closely related to the genus Dekkera (anamorph Brettanomyces). As a consequence, the name EenieZla is reduced to synonymy with the name Brettanomyces. In addition, our data do not support reassignment of certain Hanseniaspora species to the recently revived genus Kloeckeraspora.Taxonomic separation of the ascomycetous yeast genera Dekkera (anamorph Brettanomyces) and Hanseniaspora (anamorph Kloeckera) and the anamorphic ascomycetous genus Eeniella is based mainly on the following characteristics: cellular morphology, type of conidiogenesis, comparative physiology, type of coenzyme Q, G+C content, and similarities of DNA and isozymes (14,24,25,35).The genus Dekkera and its anamorph, the genus Brettanomyces, are characterized by multipolar budding (25,30,31), formation of acetic acid (4), the presence of a negative Pasteur effect (also called the Custers effect) (19)(20)(21)(22), and a coenzyme Q that contains nine isoprene units (35). Species have been defined on the basis of nuclear DNA relatedness data and the results of electrophoretic comparisons of cellular enzymes (25) and by ribotyping (15). Currently, four Brettanomyces species are distinguished: these species are Brettanomyces anomalus, Brettanomyces bruxellensis, Brettanomyces custersianus, and Brettanomyces naardenensis. Teleomorphs are known for two of these species, Dekkera anomala (anamorph B. anomalus) and Dekkera bnwcellensis (anamorph B. bruxellensis) (25).The genus Eeniella shares some characteristics with the genus Brettanomyces, including acetic acid formation, the presence of a Custers effect (20, 24), a coenzyme Q which contains nine isoprene units (359, and a similar mitochondria1 DNA gene order (8, 9). However, in current taxonomic treatments, the genus Eeniella is recognized as a genus distinct from the genus Brettanomyces because it exhibits enteroblastic bipolar budding similar to that observed in members of the genus Hanseniaspora (24, 25).The genus Hanseniaspora is characterized by apiculate cells with percurrent, bipolar budding (23) and a coenzyme Q which contains six isoprene units (33,34). Species are defined on the basis of levels of nuclear DNA relatedness (14), and the species have been supported by the results of partial 18s and 26s rRNA nucleotide sequence analyses (33, 34). However, the genus Hanseniaspora is heterogeneous with respect to ascospore morphology (23), serology (28), and proton magnetic resonance spectra of cell wall mannans (26). In their study of partial 18s and 26s rRNA nucleotide sequences of the six recognized species belonging to the genus Hanseniaspora, Yamada et al. (33,34) observed that the species could be divided into two clusters. Because of this dichotomy within the genus, these a...

Staircase electrophoresis profiles of stable low-molecular-weight RNA--a new technique for yeast fingerprinting.

“…Also, according to our results (Fig. 5), Hanseniaspora, Dekkera and Saccharomyces belong to different groups, in agreement with the findings of other authors using rDNA and rRNA nucleotide sequences (Boekhout et al, 1994 ;Cai et al, 1996 ;Kurtzman, 1994). Within the fourth subgroup, Candida species cluster together.…”

“…This background makes it necessary to have available molecular techniques able to shed light on the taxonomy of these micro-organisms. Recently, molecular techniques involving rRNA and rDNA sequencing have become available for conducting phylogenetic and classification studies (Boekhout et al, 1994 ;Cai et al, 1996 ;James et al, 1997 ;Kurtzman, 1994), and RFLP and RAPD have been applied to identification (Kurtzman, 1994). For example, the sequencing of D1\D2 domains allows the rapid identification of ascomycetous yeast (Fell, 1993).…”

Identification ofDekkera bruxellensis(Brettanomyces) from Wine by Fluorescence In Situ Hybridization Using Peptide Nucleic Acid Probes

“…In addition, the relatively hydrophobic character of PNAs compared to DNA oligonucleotides makes PNA probes capable of penetrating the hydrophobic cell wall following mild fixation conditions that do not lead to disruption of cell morphology (14). These unique characteristics of PNA have opened new possibilities for molecular diagnostic assays.The D1-D2 region of 26S ribosomal DNA (rDNA) of eucaryotic organisms shows a high degree of species variation and has been used for identification and taxonomy of yeast species (1,8). In this study, 26S rDNA sequence information was used to design species-specific probes targeting the rRNA of D. bruxellensis.…”

“…The D1-D2 region of 26S ribosomal DNA (rDNA) of eucaryotic organisms shows a high degree of species variation and has been used for identification and taxonomy of yeast species (1,8). In this study, 26S rDNA sequence information was used to design species-specific probes targeting the rRNA of D. bruxellensis.…”

Caracterização e comportamento fermentativo de linhagens de Dekkera contaminantes da fermentação alcoólica

“…O nome Brettanomyces é uma alusão ao termo British, devido ao uso de espécies deste gênero na elaboração de cerveja de aroma forte (FRANSON, 2001 CLARK-WALKER, 1986;BOEKHOUT et al, 1994).…”