Phylogeographic Structure in Anastrepha ludens (Diptera: Tephritidae) Populations Inferred With mtDNA Sequencing

Ruiz‐Arce, Raul; Owen, Christopher L.; Thomas, Donald B.; Barr, Norman B.; McPheron, Bruce A.

doi:10.1093/jee/tov082

Cited by 24 publications

(35 citation statements)

References 58 publications

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“…However, this pattern was driven by the distribution of low‐frequency haplotypes, and the most prevalent haplotype (found in 65% of individuals) was geographically widespread. While we do observe some differentiation between the northern and southern parts of the distribution (comparing Mexico to Costa Rica/Panama), similar to that identified by Ruiz‐Arce et al (), the large zone of intergradation in Central America fails to support a strong north–south divide for this species.…”

Section: Discussionsupporting

confidence: 83%

“…We then included 40 specimens, mostly of unknown origin, to act as a real‐world test of this diagnostic panel (Table , Table ). This included flies and maggots collected as part of regular monitoring efforts in California and Texas, as well as seven and two specimens from Mexico and Panama, respectively, which were specimens with questionable origins as determined with mtDNA in Ruiz‐Arce et al (). Finally, we included four reference, four alternate, and three heterozygote positive controls at varying concentrations, and a no‐template control to complete this 96‐specimen test dataset.…”

Section: Resultsmentioning

confidence: 99%

“…Compared to previous population genetic studies, this degree of population structuring is quite high. Using two mitochondrial genes, Ruiz‐Arce et al () found some support for genetic structure between the northern and southern parts of A. ludens ’ range, corresponding to the Isthmus of Tehuantepec (the narrowest point of Mexico). However, this pattern was driven by the distribution of low‐frequency haplotypes, and the most prevalent haplotype (found in 65% of individuals) was geographically widespread.…”

Section: Discussionmentioning

confidence: 99%

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Range‐wide population genomics of the Mexican fruit fly: Toward development of pathway analysis tools

Dupuis

Ruiz‐Arce

Barr

et al. 2019

Evolutionary Applications

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Recurrently invading pests provide unique challenges for pest management, but also present opportunities to utilize genomics to understand invasion dynamics and inform regulatory management through pathway analysis. In the southern United States, the Mexican fruit fly Anastrepha ludens is such a pest, and its incursions into Texas and California represent major threats to the agricultural systems of those regions. We developed a draft genome assembly for A. ludens , conducted range‐wide population genomics using restriction site‐associated DNA sequencing, and then developed and demonstrated a panel of highly differentiated diagnostic SNPs for source determination of intercepted flies in this system. Using 2,081 genomewide SNPs, we identified four populations across the range of A. ludens , corresponding to western Mexico, eastern Mexico/Texas, Guatemala/Belize/Honduras, and Costa Rica/Panama, with some intergradation present between clusters, particularly in Central America. From this population genomics framework, we developed a diagnostic panel of 28 highly differentiated SNPs that were able to recreate the genomewide population structure in this species. We demonstrated this panel on a set of test specimens, including specimens intercepted as part of regular trapping surveillance in Texas and California, and we were able to predict populations of origin for these specimens. This methodology presents a highly applied use of genomic techniques and can be implemented in any group of recurrently invading pests.

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Section: Discussionsupporting

confidence: 83%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Range‐wide population genomics of the Mexican fruit fly: Toward development of pathway analysis tools

Dupuis

Ruiz‐Arce

Barr

et al. 2019

Evolutionary Applications

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“…These values were higher than those reported for A. striata in southwestern Colombia (polymorphic sites: 14, Hd = 0.51, π = 0.0005) (Gallo‐Franco et al., ) and similar to those reported for Anastrepha ludens (Loew) through their distribution range (polymorphic sites: 105, Hd = 0.576), although nucleotide diversity was ca. 10× lower for that species (π = 0.0018) (Ruiz‐Arce et al., ). These Anastrepha species represent different eating habits that may influence their genetic diversity.…”

Section: Discussionmentioning

confidence: 96%

Genetic diversity and population structure of Anastrepha obliqua in southwestern Colombia

Aguirre-Ramirez

Velasco‐Cuervo

Gallo-Franco

et al. 2017

Entomologia Exp Applicata

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The West Indian fruit fly, Anastrepha obliqua (Macquart) (Diptera: Tephritidae), is a quarantine pest in Colombia that infests economically important plants, although little is known about its population dynamics. In this study, cytochrome oxidase subunit I (COI) and NADH dehydrogenase subunit 6 (ND6) mitochondrial genes were concatenated to characterise the genetic diversity and population structure of A. obliqua, associated with two factors: (1) ecosystem differences in two geographical regions of southwestern Colombia (the inter-Andean valley of the Cauca River and the mountain region), and (2) the host plants present in the area. Additionally, a first approach was made at understanding the species demographic history. Seven haplotypes were found with Kimura 2-parameter (K2P) genetic distances between 0.1 and 4%. Haplotype genealogies and demographic analyses suggest that the population of A. obliqua in southwestern Colombia is the result of introductory events of multiple populations of A. obliqua. However, the data indicate that the population genetic structure could be related to the ecological differences of the two regions being studied. Significant differences were also found among the distribution of haplotype frequencies of A. obliqua with regard to the diversity of host plants. This study is the first to provide an understanding of the population dynamics of A. obliqua in Colombia, which may ultimately contribute to strategies, such as sterile insect technique (SIT), for the management of the pest.

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“…It is also difficult to understand why and how these two populations diverged. In fact, the Mexican morphotype of the A. fraterculus species complex extends from northern Mexico to Panama (Hernández‐Ortiz et al., , ), and Mexican fruit fly ( Anastrepha ludens ) populations, from Mexico to Central America appear to be relatively homogeneous (Ruiz‐Arce, Owen, Thomas, Barr, & McPheron, ). Perhaps, as suggested by Scally et al.…”

Section: Discussionmentioning

confidence: 99%

Reproductive compatibility among Mexican populations of Anastrepha obliqua: theoretical and management implications

Rull

Tadeo

Lasa

et al. 2018

J Applied Entomology

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The fraterculus species group, composed of 34 species in the genus Anastrepha (Diptera: Tephritidae), includes the fraterculus cryptic species complex formed by eight reproductively isolated morphotypes. A previous study revealed six genetic mitochondrial types of Anastrepha obliqua, suggesting the existence of a second cryptic species complex. However, marked discrepancies between nuclear and mitochondrial loci rather suggest incomplete lineage sorting or introgression between A. obliqua and A. fraterculus. Such hybridization could nevertheless result in reproductive isolation, an outcome that could affect efficacy of area‐wide management for the most important pest of mangos in America. Two mitochondrial types occur in Mexico, and the limits of a third one, encompassing Central American populations, have not been clearly established. Here, we tested reproductive compatibility among three A. obliqua populations from the Pacific and a population from the Gulf of Mexico. No evidence of pre‐zygotic isolation was found. Flies from the Atlantic mated randomly for equal duration with individuals from three Pacific populations. Homotypic and heterotypic crosses produced similar numbers of eggs, with heterotypic crosses of Pacific males and Atlantic females hatching in lower proportions. Larvae of all cross types developed equally in mangos and exhibited no sex ratio distortion of hybrid F1. The three mitochondrial types identified in Mexico and Central America do not appear to be cryptic species and can be managed using one single strain for the sterile insect technique.

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Phylogeographic Structure in Anastrepha ludens (Diptera: Tephritidae) Populations Inferred With mtDNA Sequencing

Cited by 24 publications

References 58 publications

Range‐wide population genomics of the Mexican fruit fly: Toward development of pathway analysis tools

Range‐wide population genomics of the Mexican fruit fly: Toward development of pathway analysis tools

Genetic diversity and population structure of Anastrepha obliqua in southwestern Colombia

Reproductive compatibility among Mexican populations of Anastrepha obliqua: theoretical and management implications

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