Phylogroup classification and investigation the relationships between phylogroups and antibiotic resistance patterns of uropathogenic E. coli isolated from pediatric urinary tract infection

Moez, Narjes Morovati; Mashouf, R Yousefi; Sedighi, Iraj; Shokoohizadeh, Leili; Taheri, Mohammad

doi:10.1016/j.genrep.2020.100758

Cited by 9 publications

(5 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In Ethiopia, UTI was mainly caused by group B2 (30%) followed by group D (27.5%), group B1 (24%) and group A (18.5%). 22 In agreement, literatures from Mongolia, 30 Poland 36 and Iran 37 have stated that group B2 is the most prevalent E. coli strains. On the other hand, phylogroup D was the most prevalent group causing UTI in China.…”

Section: Discussionsupporting

confidence: 69%

“…Different data are available about the relationship of antibiotic resistance profile with specific phylogroups such as A, 41 , 43 B2 3 , 42 , 44 and E. 37 This variation in the distribution of the antibiotic resistance between UPEC phylogenetic groups might be due to different dominant phylogroups in the different geographic regions even within the same country. 37 , 44 No significant association was detected between different phylogenetic groups and MDR in accordance with other literatures. 20 , 25 , 30 No relevance was detected in the phylogenetic groups with respect to the biofilm formation in contrary to other literatures where phylogroups A 20 and B2 45 have been associated with more biofilm production.…”

Section: Discussionmentioning

confidence: 99%

“…20,25,30 Additionally, in Mexico, the high antibiotic resistance was distributed in all phylogenetic groups. 42 Different data are available about the relationship of antibiotic resistance profile with specific phylogroups such as A, 41,43 B2 3,42,44 and E. 37 This variation in the distribution of the antibiotic resistance between UPEC phylogenetic groups might be due to different dominant phylogroups in the different geographic regions even within the same country. 37,44 No significant association was detected between different phylogenetic groups and MDR in accordance with other literatures.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Characterization of E. coli Phylogroups Causing Catheter-Associated Urinary Tract Infection

El-Mahdy

Mahmoud

Shrief

2021

IDR

View full text Add to dashboard Cite

Purpose Characterization of different uropathogenic E. coli (UPEC) phylogroups is crucial to understand pathogenesis of urinary tract infection (UTI). The objective of our study was to evaluate the antibiotic resistance pattern, biofilm formation and pathogenicity islands (PAIs) of UPEC phylogroups isolated from catheter-associated UTI (CAUTI) compared to community UTI (Com-UTI). Patients and Methods This study included 90 UPEC strains recovered from CAUTI and Com-UTI. Antimicrobial susceptibility was tested by the Kirby–Bauer method and extended spectrum beta-lactamase (ESBL) production was confirmed using the combined disk. The biofilm formation was tested using the microtiter plate assay. Main E. coli phylogroups (A, B1, B2 and D) were detected by multiplex PCR and 2 multiplex PCR detected the 8 PAIs. Results Antibiotic resistance of UPEC strains showed a similar high resistance in CAUTI and Com-UTI. Isolates from CAUTI significantly produced biofilm higher than Com-UTI strains (68.9% vs 44.4%). In CAUTI and Com-UTI isolates, phylogroup A was the commonest (53.3% vs 48.9%, respectively). PAI IV536 was the most common in the strains from CAUTI (71.1%) and Com-UTI (73.3%). No significant relationship was detected between the studied characters and different phylogroups except the significant resistance to cefotaxime, ceftazidime and aztreonam among phylogroups from CAUTI isolates. Conclusion Increased antibiotic resistance and ESBLs were detected in UPEC strains from CAUTI and Com-UTI. The strains from CAUTI significantly produced biofilm higher than Com-UTI strains. Phylogroup A was the predominate phylogroup and PAI IV536 was the most prevalent marker in all phylogroups from both types of UTI.

show abstract

Section: Discussionsupporting

confidence: 69%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Characterization of E. coli Phylogroups Causing Catheter-Associated Urinary Tract Infection

El-Mahdy

Mahmoud

Shrief

2021

IDR

View full text Add to dashboard Cite

show abstract

“…Most of the bacteraemia isolates belonged to the phylogroup B2 (Table 3.2) and two sequence types ST131 and ST73 predominated (Figure 3.3). This is in line with reports of other bacteraemia collections as B2 is the most identified phylogroup [504][505][506], and ST131 and ST73 being the most common sequence types in bacteraemia isolates [507,508].…”

Section: Results Chapter Summaries Chaptersupporting

confidence: 92%

Identifying Host and Bacterial Biomarkers to Predict Sepsis

Lewis

View full text Add to dashboard Cite

Sepsis is a serious concern for healthcare programmes worldwide and is associated with 11 million deaths annually. E. coli accounts for 20% of bloodstream infections worldwide and is responsible for 17% of sepsis related mortality. Early diagnosis and treatment are critical and delays in initiating antimicrobial therapy are linked to mortality. Identifying biomarkers to predict patients that might succumb to sepsis is vital to aid in early diagnosis. It was hypothesised that E. coli bacteraemia isolates and isolates from different sources of bacteraemia would elicit a distinctive host response and be genetically unique. Blood culture positive isolates (n=165) were collected from the Hywel Dda University Health Board. Most of the isolates were assigned to the B2 and D phylogroups and belonged to either ST131 or ST73. Antimicrobial resistance in the collection was lower than national averages. Host models of infection were used to identify phenotypic responses of the bacterial collection. IL-8 and MIP3α were increased following stimulation by bacteraemia isolates compared to non-pathogenic strains. Greater IL-8 in whole blood was associated with a urinary and abdominal bacteraemia. Isolates that were resistant to human plasma elicited a higher IL-6, IL-8 and resistin response in whole blood compared to plasma sensitive isolates. Blood culture positive bacteraemia isolates had significantly more virulence factors than control isolates. Bacteraemia isolates expressed more P fimbriae genes. The S fimbrial adhesin genes were found to be significantly different between urinary and abdominal isolates. Abdominal isolates had significantly more sfaC (32%) while urinary isolates had more sfaX (22%). GWAS analysis revealed 6 potential gene targets based on bacterial phenotypes. These were ynbC, yhgE, ybjE, yejF, tufB and yohF. The results contained within this thesis describe new targets for predicting bacteraemia and sepsis and underline the importance of using host and pathogen as sources for biomarkers.

show abstract

“…Based on our comprehensive search, 28 studies with eligibility criteria (Figure 1) were included in the meta-analysis (Ramos et al, 2011;Navidinia et al, 2013;Adib et al, 2014;Alizade et al, 2014a;Alizade et al, 2014b;Hemati et al, 2014;Kazemnia et al, 2014;Derakhshandeh et al, 2015;Iranpour et al, 2015;Rahdar et al, 2015;Salmani et al, 2016;Sohrabi and Zeighami, 2016;Hashemizadeh et al, 2017;Hojabri et al, 2017;Salehzadeh and Zamani, 2018;Bahadori et al, 2019;Farajzadah Sheikh et al, 2019;Norouzian et al, 2019;Shahin et al, 2019;Staji et al, 2019;Bakhtiari et al, 2020;Moez et al, 2020;Ranjbar et al, 2020;Yazdanpour et al, 2020). Of the 28 included studies, 17 and 9 studies reported the prevalence of phylogenic groups from hospitalized and community patients, respectively.…”

Section: Database Search and Characterization Of Studiesmentioning

confidence: 99%

Phylogenetic Group Distribution of Uropathogenic Escherichia coli and Related Antimicrobial Resistance Pattern: A Meta-Analysis and Systematic Review

Halaji

Fayyazi

Rajabnia

et al. 2022

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

The phylogenetic classification of Escherichia coli isolates is of great importance not only for understanding the populations of E. coli but also for clarifying the relationship between strains and diseases. The present study aimed to evaluate the prevalence of phylogenetic groups, antibiotic susceptibility pattern, and virulence genes among uropathogenic E. coli (UPEC) isolated from different parts of Iran through a systematic review and meta-analysis. Several international electronic sources, including Web of Science, PubMed, Scopus, and Embase, were searched (2000–2020) in order to identify the studies compatible with our inclusion criteria. The meta-analysis was performed using the metaprop program in the STATA (version 11) software. Based on our comprehensive search, 28 studies meeting the eligibility criteria were included in the meta-analysis. The pooled prevalence of phylogroups B2, D, B1, and A was 39%, 26%, 18%, and 8%, respectively. In addition, there was a significant heterogeneity among different phylogroups. However, according to the results of Begg’s and Egger’s tests, there were no significant publication bias in phylogroups B2, D, B1, and A. This research provided the first comprehensive study on phylogroups of UPEC isolated in Iran. Our findings indicated that phylogroup B2 and group D were the most predominant phylogenetic groups among UPEC isolates in various regions of Iran. In addition, we observed that certain phylogenetic groups are more antibiotic resistant than the others. It was also observed that the dissemination of virulent phylogroup B2 and D should be controlled via comprehensive infection control measures. Additionally, certain strategies should be developed for monitoring the antibiotic therapy.

show abstract

Phylogroup classification and investigation the relationships between phylogroups and antibiotic resistance patterns of uropathogenic E. coli isolated from pediatric urinary tract infection

Cited by 9 publications

References 37 publications

Characterization of E. coli Phylogroups Causing Catheter-Associated Urinary Tract Infection

Characterization of E. coli Phylogroups Causing Catheter-Associated Urinary Tract Infection

Identifying Host and Bacterial Biomarkers to Predict Sepsis

Phylogenetic Group Distribution of Uropathogenic Escherichia coli and Related Antimicrobial Resistance Pattern: A Meta-Analysis and Systematic Review

Contact Info

Product

Resources

About