Physical and antibacterial properties of bacterial cellulose films supplemented with cell-free supernatant enterocin-producing Enterococcus faecium TJUQ1

Feng, Yulong; Yin, Nan; Zhou, Zhansong

doi:10.1016/j.fm.2021.103828

Cited by 14 publications

(3 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was shown, that CFS and semipurified BLIS produced by Enterococcus hirae ST57ACC have no cytotoxic effect against HT-29 cells (Cavicchioli et al, 2019). Cell-free supernatant of bacteriocinogenic E. faecium TJUQ1 strain was used for the preparation of composite film with antibacterial activity (Feng et al, 2021). But the safety of each purified bacteriocin needs to be assessed before use in the food industry.…”

Section: Discussionmentioning

confidence: 99%

Screening of bacteriocin-producing dairy Enterococcus strains using low-cost culture media

Garmasheva

Oleschenko

2023

Front. Microbiol.

View full text Add to dashboard Cite

This study was carried out to select the bacteriocinogenic strains among Enterococcus strains isolated from Ukrainian traditional dairy products using a low-cost media for screening, that containing molasses and steep corn liquor. A total of 475 Enterococcus spp. strains were screened for antagonistic activity against Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, and Listeria monocytogenes indicator strains. The initial screening revealed that 34 Enterococcus strains during growth in low-cost medium containing corn steep liquor, peptone, yeast extract, and sucrose produced metabolites with inhibition activity against at least of the indicator strains used. Enterocin genes entA, entP, and entB were detected in 5 Enterococcus strains by PCR assay. Genes of enterocins A and P were found in E. faecalis 58 and Enterococcus sp. 226 strains, enterocins B and P – in Enterococcus sp. 423, enterocin A – in E. faecalis 888 and E. durans 248 strains. Bacteriocin-like inhibitory substances (BLIS) produced by these Enterococcus strains were thermostable and sensitive to proteolytic enzymes. To our knowledge, this is the first report on the isolation of enterocin-producing wild Enterococcus strains from traditional Ukrainian dairy products using a low-cost media for screening bacteriocinogenic strains. Strains E. faecalis 58, Enterococcus sp. 423, and Enterococcus sp. 226 are promising candidates for practical use as producers of bacteriocins with inhibitory activity against L. monocytogenes using molasses and steep corn liquor as cheap sources of carbon and nitrogen, that can significantly reduce the cost of industrial bacteriocin production. Further studies will be required to determine the dynamic of bacteriocin production, its structure, and mechanisms of antibacterial action.

show abstract

Section: Discussionmentioning

confidence: 99%

Screening of bacteriocin-producing dairy Enterococcus strains using low-cost culture media

Garmasheva

Oleschenko

2023

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Hai et al [24] used bamboo nanofibers and chitin to prepare a new type of nanocomposite with good thermal stability, suitable tensile strength, and complete biodegradation. Feng et al [25] used bacterial cellulose to prepare a composite membrane that had a significant inhibitory effect on Listeria monocytogenes. Wang Tingting et al [26] loaded rhodopsin on bacterial cellulose/chitosan composite materials to prepare a nanocellulose film with antibacterial function.…”

Section: Discussionmentioning

confidence: 99%

Research on Cellulose Film in Degradable Wet Garbage Bags

Yinxuan

2022

PSPRJ

View full text Add to dashboard Cite

Cellulose is a natural polymer compound with the largest reserves in nature and is considered to be the main raw material for energy and chemical industry in the future. However, the high degree of polymerization and high crystallinity of natural cellulose make it difficult to dissolve in conventional solvents, which greatly limits the application of cellulose. In recent years, a variety of new types of cellulose have been discovered, and this paper briefly introduces plant-derived cellulose materials. The cellulose-based composite film obtained by dissolving the cellulose solvent system has been improved to a certain extent in terms of strength and degradability and is expected to be applied to the problem of wet garbage bag breaking caused by garbage classification in recent years. At present, cellulose-based composite materials have been widely used in food preservation and antibacterial packaging materials, active packaging materials and high barrier packaging materials. Based on the latest research on cellulose membrane materials, this paper looks forward to the future development hotspots, aiming to provide references for cellulose dissolution and the development of new cellulose membrane materials.

show abstract

“…Nata de coco is a traditional dessert which has been firstly fermented in waste coconut water in southeast Asian countries such as Philippines, Indonesia and Vietnam. Nata de coco has gained extensive popularity owing not only as a weight-loss food with good elastic taste, low caloric and high fiber contents [1], but also its excellent material properties in the field of biomaterials [2]. In fact, nata de coco is a gelatinous microbial extracellular polysaccharide consisting of linear β-1,4-D-glucose units, widely known as bacterial cellulose (BC).…”

Section: Introductionmentioning

confidence: 99%

Insights into Proteomics Reveal Mechanisms of Ethanol-Enhanced Bacterial Cellulose Biosynthesis by Komagataeibacter nataicola

Fei

Yang

et al. 2023

Fermentation

View full text Add to dashboard Cite

Nata de coco, known as bacterial cellulose (BC), has been given much attention in the food industry and biomaterial areas due to its specific properties such as low calorie content, high content of fiber, high purity and high biocompatibility. Komagataeibacter spp. are indispensable microorganisms for BC production due to their highly efficient production. Here, proteomics was applied to investigate the metabolism regulation mechanisms of BC yield improvements in K. nataicola Y19 by 48 ± 3% after ethanol supplementation. The results evidenced that differentially expressed proteins involved in the BC biosynthesis system, glycolytic pathway, TCA cycle and oxidative phosphorylation process were up-regulated. The proteins accelerated the BC biosynthesis by providing more energy and via intermediate metabolites. Furthermore, the elongation factor Tu, chaperone DnaK and translocase subunit SecB may be involved in the BC synthesis procedure by regulating electron transfer, hydrolysis of ATP and protein transformation. Moreover, the ethanol-enhanced BC biosynthesis may be associated with the decreased expression of endoglucanase. This research elucidates the proteomics mechanism of higher BC production based on ethanol addition, providing references for nata de coco production efficiency and the synthetic regulation of bacterial cellulose in the future.

show abstract

Physical and antibacterial properties of bacterial cellulose films supplemented with cell-free supernatant enterocin-producing Enterococcus faecium TJUQ1

Cited by 14 publications

References 38 publications

Screening of bacteriocin-producing dairy Enterococcus strains using low-cost culture media

Screening of bacteriocin-producing dairy Enterococcus strains using low-cost culture media

Research on Cellulose Film in Degradable Wet Garbage Bags

Insights into Proteomics Reveal Mechanisms of Ethanol-Enhanced Bacterial Cellulose Biosynthesis by Komagataeibacter nataicola

Contact Info

Product

Resources

About