Introduction: Application of lactic acid bacteria for synthesis of silver (AG) nanoparticles (NPs) could be a good ecological friendly alternative to chemical and physical methods. The objective of this study was to investigate the biosynthesis of silver NPs using Lactobacillus strains and to compare their monosaccharide composition of capsular exopolysaccharides and the antibacterial activity of synthesized nanoparticles. Methods: The washed cells of 22 Lactobacillus strains were used for in vitro silver nanoparticle biosynthesis from silver nitrate solution. The NPs formation was confirmed by UV-visible spectroscopy and transmission electron microscopy (TEM) analysis. TEM micrographs were used for the evaluation of NPs size. The monosaccharide composition of capsular exopolysaccharides was determined using GC/MS analysis. The antimicrobial activity was determined by agar well diffusion assay. Results: The capsular layers of Lactobacillus strains contained heteropolysaccharides that were composed mostly of glucose, mannose, galactose and rhamnose in a different molar ratio. It was found that Ag NPs with large size (30.65 ± 5.81 nm) obtained from L. acidophilus 58p were more active against S. epidermidis, E. coli, K. pneumonia,S. flexneri and S. sonnei compared with Ag NPs from L. plantarum 92T (19.92 ± 3.4 nm). Conclusion: The size and antibacterial activities of Ag NPs were strain-dependent and such characteristics may be due to the capsular biopolymer composition of Lactobacillus strains used for Ag NPs synthesis.

This study was carried out to select the bacteriocinogenic strains among Enterococcus strains isolated from Ukrainian traditional dairy products using a low-cost media for screening, that containing molasses and steep corn liquor. A total of 475 Enterococcus spp. strains were screened for antagonistic activity against Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, and Listeria monocytogenes indicator strains. The initial screening revealed that 34 Enterococcus strains during growth in low-cost medium containing corn steep liquor, peptone, yeast extract, and sucrose produced metabolites with inhibition activity against at least of the indicator strains used. Enterocin genes entA, entP, and entB were detected in 5 Enterococcus strains by PCR assay. Genes of enterocins A and P were found in E. faecalis 58 and Enterococcus sp. 226 strains, enterocins B and P – in Enterococcus sp. 423, enterocin A – in E. faecalis 888 and E. durans 248 strains. Bacteriocin-like inhibitory substances (BLIS) produced by these Enterococcus strains were thermostable and sensitive to proteolytic enzymes. To our knowledge, this is the first report on the isolation of enterocin-producing wild Enterococcus strains from traditional Ukrainian dairy products using a low-cost media for screening bacteriocinogenic strains. Strains E. faecalis 58, Enterococcus sp. 423, and Enterococcus sp. 226 are promising candidates for practical use as producers of bacteriocins with inhibitory activity against L. monocytogenes using molasses and steep corn liquor as cheap sources of carbon and nitrogen, that can significantly reduce the cost of industrial bacteriocin production. Further studies will be required to determine the dynamic of bacteriocin production, its structure, and mechanisms of antibacterial action.

Nanosized composites CeO2–Ag, La2O3–Ag, and TiO2–Ag are a class of nanomaterials suitable for photocatalysis, optical devices, and photoelectrochemical elements. Further, nanocomposites with several wt.% of silver can be used as creating materials for pathogenic virus inactivation with pandemic-neutralizing potential. Thus, CeO2–Ag, La2O3–Ag, and TiO2–Ag nanocomposites are prospective materials due to their optical and biological activity. In the present work, CeO2–Ag, La2O3–Ag, and TiO2–Ag nanocomposites were synthesized by the co-precipitation method. The morphological and optical properties and the structure of the prepared nanocomposites were analyzed using X-ray diffraction (XRD), scanning electron microscopy (SEM) with EDX, and nitrogen adsorption-desorption based on BET, Raman spectroscopy, and photoluminescence (PL). Both oxide matrixes corresponded to the cubic crystal lattice with the inclusion of argentum into the crystal lattice of oxides at relative low c(Ag) and reduction of silver on particle surface at 5 wt.% Ag and greater. The CeO2, TiO2, and La2O3 with a concentration of 4 wt.% Ag inhibited the growth processes of prokaryotic cells of E. coli, Bacillus sp., and S. aureus compared to pure oxides. Influenza A virus and herpes completely suppressed reproduction by nanocomposites of CeO2–Ag (2, 5 wt.%) and La2O3–Ag (2, 5 wt.%) action.

Cellular fatty acids composition is an important chemotaxonomic characteristic of bacterial cells. At the same time cellular fatty acids play a key role in maintaining the viability of micro-organisms in different environmental conditions. In this study, intraspecies heterogeneity of cellular fatty acids composition of Lactobacillus plantarum strains was examined. This work provides novel and important information about a relationship between cellular fatty acids composition of Lact. plantarum strains and source of isolation or stress resistance profile. Our results showed that cellular fatty acids composition is quite diverse among Lact. plantarum strains derived from different sources and may reflect previous cell's history. Our findings should be considered in chemotaxonomic studies of lactic acid bacteria and its ecology.

No abstract