Physical and biological performances of a semi-resorbable barrier membrane based on silk fibroin-glycerol-fish collagen material for guided bone regeneration

Abstract

The fragility of silk fibroin film is a drawback to being used as a barrier membrane. Semi-resorbable barrier membranes maintain function longer than a resorbable membrane and no need to be removed. The study aimed to fabricate semi-resorbable membranes using silk fibroin with glycerol plasticizer (Group A), immobilized with fish collagen (Group B), and then characterized, in vitro biocompatibility tested, and compared with a commercial collagen membrane (Group C). Group B showed more roughness (0.2155 µm) than Group A (0.1424 µm). Group A was more hydrophilic (76.75° ± 3.07°) and more stiffness (28.93% ± 15.56%) than Group B (112.67° ± 1.94°, 42.10% ± 11.46%) and C (54.79% ± 13.44%) without significant difference. Group C had a significantly higher ( p < 0.05) swelling degree and less degradation rate than others. Group A showed significantly highest ( p < 0.05) cell proliferation. Group C showed more alkaline phosphatase activity than others but no significant difference in osteocalcin and Alizarin Red activity on day 21. The semi-resorbable membrane based on silk fibroin-glycerol possessed good physical and mechanical properties, and well-supported osteoblastic cell proliferation and differentiation.

Anisotropic silk nanofiber layers as regulators of angiogenesis for optimized bone regeneration

“…Silk fibroin (SF) biomaterials are promising bone regenerative matrices due to the biocompatibility, excellent and tunable mechanical properties, and ease of materials fabrication [ [9] , [10] , [11] , [12] ]. SF-based scaffolds, films and hydrogels have been developed to repair various bone defects [ 9 , [13] , [14] , [15] ]. The cytocompatibility and bioactivity of these matrices were further improved by changing nano-micro structures, anisotropic topography, degradation behavior and mechanical performance [ [16] , [17] , [18] , [19] ].…”

The efficacy of a semi-resorbable membrane based on silk fibroin-Glycerol on bone regeneration in rabbit calvarial defects compared to a commercial collagen membrane

“…These characteristics were consistent with the hydrophobicity of membranes. 20 The OD from the 2D digital radiograph at 4-weeks of the BG group was lower than the E group, but not statistically different, this might be the consequence of the collapse of the collagen membrane into the defect area and interrupted the bone formation in the early stage.…”

“…Recently, silk fibroin and fish collagen-based materials with a glycerol plasticizer had been fabricated and characterized to overcome the brittleness and low mechanical strength of silk fibroin material for a barrier membrane and to diminish religious restrictions from the mammal sources. 20 The in vitro study showed that the silk fibroin-glycerol and silk fibroin-glycerol-collagen membranes had good physical and mechanical properties with high stiffness 1.5–2.2 times that of the collagen membrane but still yielded sufficient elongation when compared to the collagen membrane, particularly the silk fibroin-glycerol-collagen. 20 The constructed membrane does not easily collapse, so it can maintain space for bone regeneration with slow resorption and has good biocompatibility to promote cell viability and cell proliferation.…”

“…20 The in vitro study showed that the silk fibroin-glycerol and silk fibroin-glycerol-collagen membranes had good physical and mechanical properties with high stiffness 1.5–2.2 times that of the collagen membrane but still yielded sufficient elongation when compared to the collagen membrane, particularly the silk fibroin-glycerol-collagen. 20 The constructed membrane does not easily collapse, so it can maintain space for bone regeneration with slow resorption and has good biocompatibility to promote cell viability and cell proliferation. The previous study showed that the silk fibroin-glycerol membrane presented better osteoblastic cell proliferation and differentiation than the collagen membrane, while the silk firoin-glycerol-collagen membrane gave comparable results to the collagen membrane.…”

Intrinsically Conductive Bifunctional Nanocellulose-Reinforced Robust and Self-Healable Electronic Skin: Deep Insights into Multiple Bonding Network, Property Reinforcement, and Sensing Mechanism

“…In Figure a, the film has a specific stability and can maintain the film morphology for 3 days under the environment of 22 °C and RH = 66% and gelatinize on the 6th day. This behavior is due to the dual role of calcium ion’s excellent water molecule capture properties it can capture water molecules from the air , and glycerin’s water-retaining function making the composites gelled. When the gel material was attached to the glove to detect electrical signals from a finger bending, more sensitive resistive signals were found.…”