Physical and Biological Properties of a Novel Hydrogel Composite Based on Oxidized Alginate, Gelatin and Tricalcium Phosphate for Bone Tissue Engineering

Cai, Kaiyong; Zhang, Jun; Deng, Lin; Yang, Li; Hu, Yan; Chen, Cong; Xue, Lei; Wang, Lu

doi:10.1002/adem.200700222

Cited by 46 publications

(28 citation statements)

References 33 publications

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“…This is one of the most important properties of hydrogel based biomaterials. 36,55 As shown in Fig. 9, the swelling ratio of ADA microcapsules decreased signicantly compared to alginate microcapsules.…”

Section: Swelling Behavior Of Microcapsulesmentioning

confidence: 98%

Fabrication of alginate–gelatin crosslinked hydrogel microcapsules and evaluation of the microstructure and physico-chemical properties

et al. 2014

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Microencapsulation of cells by using biodegradable hydrogels offers numerous attractive features for a variety of biomedical applications including tissue engineering. This study highlights the fabrication of microcapsules from an alginate-gelatin crosslinked hydrogel (ADA-GEL) and presents the evaluation of the physico-chemical properties of the new microcapsules which are relevant for designing suitable microcapsules for tissue engineering. Alginate di-aldehyde (ADA) was synthesized by periodate oxidation of alginate which facilitates crosslinking with gelatin through Schiff's base formation between the free amino groups of gelatin and the available aldehyde groups of ADA. Formation of Schiff's base in ADA-GEL and aldehyde groups in ADA was confirmed by FTIR and NMR spectroscopy, respectively. Thermal degradation behavior of films and microcapsules fabricated from alginate, ADA and ADA-GEL was dependent on the hydrogel composition. The gelation time of ADA-GEL was found to decrease with increasing gelatin content. The swelling ratio of ADA-GEL microcapsules of all compositions was significantly decreased, whereas the degradability was found to increase with the increase of gelatin ratio. The surface morphology of the ADA-GEL microcapsules was totally different from that of alginate and ADA microcapsules, observed by SEM. Two different buffer solutions (with and without calcium salt) have an influence on the stability of microcapsules which had a significant effect on the gelatin release profile of ADA-GEL microcapsules in these two buffer solutions.

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Section: Swelling Behavior Of Microcapsulesmentioning

confidence: 98%

Fabrication of alginate–gelatin crosslinked hydrogel microcapsules and evaluation of the microstructure and physico-chemical properties

et al. 2014

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“…They resemble extracellular matrices of tissue comprised of various amino acids and sugars based macromolecules thus have the potential to direct the migration, growth, and organization of cells during the tissue regeneration 17, 18. A hydrogel derived from alginate dialdehyde (ADA) and gelatin is an in situ forming biodegradable polymer of well‐known biocompatibility and bioresorbability without employing any extraneous cross‐linking agent 19. However, rapid cross‐linking and gelation is possible between oxidized alginate and gelatin in the presence of borax 20.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of alginate dialdehyde cross‐linked gelatin hydrogel as a biodegradable sealant for polyester vascular graft

et al. 2011

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Vascular grafts are devices intended to replace compromised arteries in the body and grafts made of polyethylene terephthalate (PET) fabric have been used mainly for synthetic grafting procedures involving medium to large diameter vascular grafts. Though porosity of the graft permits tissue in-growth, it would lead to bleeding through the graft walls immediately after implantation. So it is essential to seal the pores either by preclotting with patient's own blood or by other sealing materials prior to implantation in order to prevent blood leakage through the graft wall. Biodegradable hydrogel materials are ideal candidates for this purpose. Apart from sealing the pores, they offer biocompatible and low-thrombogenic surfaces when coated on vascular graft. In the present study, a biodegradable hydrogel, derived from oxidized alginate and gelatin, has been deposited on PET grafts by dip coating and were characterized for its efficacy on sealing the pores of the graft. Water permeability in the static and pulsatile conditions, burst strength, in vitro cell culture cytotoxicity, hemocompatibility, and endothelial cell adhesion and proliferation of the coated grafts were investigated. Results showed that the alginate dialdehyde cross-linked gelatin hydrogel was nontoxic, hemocompatible, and was efficient in sealing the pores of the graft. Blood perfusion study showed that when hydrogel-coated grafts were exposed to blood for 30 min, they showed little affinity toward platelets or leukocytes. Hemolytic potential of PET was significantly reduced when it was coated with hydrogel. Improved adhesion and proliferation of endothelial cells were observed when PET grafts were coated with hydrogel. Results also showed that coating with hydrogel did not affect the burst strength of the PET graft.

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“…Sodium alginate (SA) was supplied by Sigma‐Aldrich Co., Ltd (USA), and chitosan (CS) with a degree of deacetylation of 90% was purchased from Jinan Haidebei Marine Bioengineering CO., Ltd (Shandong, China). The viscosity‐average molecular mass ( M η ) of SA and CS were determined to be 5.5 × 10 4 g mol −1 and 14.4 × 10 4 g mol −1 according to literature methods, respectively. N , N ‐dimethylacrylamide (DMAA, monomer) was purchased from Macklin Biochemical Co., Ltd (Shanghai, China).…”

Section: Methodsmentioning

confidence: 99%

Mechanical Strengths of Hydrogels of Poly(N,N‐Dimethylacrylamide)/Alginate with IPN and of Poly(N,N‐Dimethylacrylamide)/Chitosan with Semi‐IPN Microstructures

Bai

Huang

Xiao

et al. 2019

Macro Materials & Eng

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Tough hydrogels receive continuous attention because of their promising applications in many fields. Herein, tough hydrogels of poly (N,N‐dimethylacrylamide) (PDMAA)/alginate (SA) are prepared, with interpenetrating network (IPN) and of PDMAA/chitosan (CS) with semi‐IPN microstructure, respectively. The toughening of the hydrogel by incorporating natural polymers is studied by compressing tests and dynamic mechanical analyses. Moreover, cyclic load–unload compressing of the two types of hydrogels are performed at low strains and under relatively high strains, in order to compare their strength and anti‐fatigue properties. The results indicate that the mechanical strength can be markedly improved upon addition of the natural polymers, and the IPN hydrogel of PDMAA/SA reveals much higher mechanical performances but is less stable. However, the semi‐IPN hydrogel of PDMAA/CS displays excellent anti‐fatigue stability, but with relatively low strength. Swelling tests, scanning electron microscopy, and Fourier transform infrared spectroscopy are carried out to study the microstructures of the hydrogels, which are carefully analyzed to understand the difference in mechanical performances of those hydrogels. The results suggest that the presence of sacrificial unit and higher chain density in the IPN are helpful for toughening hydrogels, while the semi‐IPN network is beneficial to improve the energy dissipation efficiency.

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Physical and Biological Properties of a Novel Hydrogel Composite Based on Oxidized Alginate, Gelatin and Tricalcium Phosphate for Bone Tissue Engineering

Cited by 46 publications

References 33 publications

Fabrication of alginate–gelatin crosslinked hydrogel microcapsules and evaluation of the microstructure and physico-chemical properties

Fabrication of alginate–gelatin crosslinked hydrogel microcapsules and evaluation of the microstructure and physico-chemical properties

Evaluation of alginate dialdehyde cross‐linked gelatin hydrogel as a biodegradable sealant for polyester vascular graft

Mechanical Strengths of Hydrogels of Poly(N,N‐Dimethylacrylamide)/Alginate with IPN and of Poly(N,N‐Dimethylacrylamide)/Chitosan with Semi‐IPN Microstructures

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