The aim of this study was to measure the physicochemical properties of 28 mountain streams in Tatra National Park, Slovakia. Sampling sites (119) were selected based on a previous study conducted in 2010. Physical properties (e.g., temperature, conductivity, total dissolved solids, pH, and dissolved oxygen) and chemical components (e.g., nitrogen oxides, ammonia oxides, chloride compounds, and chemical oxygen demand) of the water were determined. Environmental parameters of streams (elevation, slope, aspect, width, depth, flow accumulation, watershed size, bedrock, and presence of mountain lakes) at sampling sites were examined. While comparing results from both periods (2010 and 2017), we found a correlation in data trends, concluding that elevation plays a significant role in almost all investigated parameters. Downstream, streams were more saturated by dissolved solids, CaCO3, and nitrates, increasing the pH level. Despite this well-known trend, we observed significant higher levels of ammonias and chlorides in the alpine zone, especially at sites where higher water temperature and lower values of dissolved oxygen were observed. This occurred in the Eastern Tatras, below mountain lakes, and where watersheds had granite origins. There are indications that denitrification processes were significantly stronger in 2017, but, on the other hand, increased chlorides resulted in stronger inhibition of nitrification processes in alpine zones at sites below mountain lakes.