Physical and physiological dormancy in black henbane (Hyoscyamus niger L.) seeds

Abstract

Aim of this study was to investigate the nature of dormancy in black henbane (Hyoscyamus niger) seeds which have low germination rate under normal laboratory conditions. To do this, before placing the seeds in Petri dishes, they were soaked in 5, 10 and 15 mg/L GA; 1, 2 and 3% H2SO4, 15 mg/L GA + 1% H2SO4, 0.01 M KNO~ solutions, tap water, 40, 50 and 60~ hot water for 30 min. The study was performed under both continuous illumination and darkness in growth chambers to evaluate the effect of light on germination rate. The results showed that H2SO4 and GA treatments were the most important factors affecting seed germination and their germination enhancing effects were more evident in darkness. The results also suggested that black henbane seeds exhibit double dormancy involving a hard seed coat and a partially dormant embryo and have a partial dark requirement to germinate. K, eywords: black henbane, dark requirement, double dormancy, CA, H2SO4

Activating antioxidant enzymes, hyoscyamine and scopolamine biosynthesis of Hyoscyamus niger L. plants with nano-sized titanium dioxide and bulk application

“…A cosmopolitan, strong-scented annual or biennial herb, which all it's parts (root, leaf, and seed) contain tropane alkaloids such as HYO and SCO (Cuneyt et al, 2004). These metabolites are synthesized in roots and then transported to the aerial parts of the plant (Oksman, 1987).…”