“…6. It was distinctly that compared with big flocs, the micro flocs in activated sludge consisting of more dispersed nitrifying microorganisms such as ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) could significantly improve denitrification due to less oxygen limitation, once they were exposed to large bulk of aerated-anoxic environment [14,33]. According to PCR-DGGE and sequencing analysis (in Table 4), furthermore, these small sized flocs were also constituted by substantial biomass including uncultured bacterium Bacteroidetes, a,e,d-proteobacteria, Chloroflexi, and Firmicutes, as well as enrichment culture bacterium, indicating the co-existing of aerobic, microaerophilic and denitrifying bacteria performing SND capacity [16].…”