Physical Characterization and Cellular Toxicity Studies of Commercial NiO Nanoparticles

Kunc, Filip; Bushell, Michael E.; Du, Xiaomei; Zborowski, Andre; Johnston, Linda J.; Kennedy, David C.

doi:10.3390/nano12111822

Cited by 8 publications

(6 citation statements)

References 34 publications

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“…The Z-average and PDI measured in the cell culture medium were very similar to the data obtained in water and the measured values were reasonably stable over a period of 72 h, which is the maximum time required for the biological assays. These results are in contrast to those obtained in our earlier work on other metal oxide NPs for which the hydrodynamic diameter and polydispersity typically increased in cell culture medium compared to water and continued to increase with storage time [ 22 , 23 ].…”

Section: Resultscontrasting

confidence: 99%

“…The agreement between measured SSA and manufacturer data was poor for more than half of the samples. It should be noted that the decrease in SSA for PVP and stearic-acid-modified NPs is consistent with our earlier results for both NiO and CeO 2 NPs [ 22 , 23 ]. In both cases, the measured SSA decreased slightly for the PVP-coated sample compared to the bare sample of the same size from the same supplier; there was a considerably larger decrease for samples containing stearic acid, which may indicate that the addition of a long chain fatty acid introduces issues with measurement of SSA.…”

Section: Resultssupporting

confidence: 92%

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Characterization and Cellular Toxicity Studies of Commercial Manganese Oxide Nanoparticles

Johnston,

Du,

Zborowski

et al. 2024

Nanomaterials

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Manganese oxide nanoparticles (MnOx NPs) are finding applications in several environmentally important areas such as farming and energy storage. MnOx NPs span a range of metal oxidation states that open up a wide range of applications in catalysis as well. As a result, it is important to understand how such materials can impact human health through incidental exposure. In this study, we examined a range of commercially available Mn2O3 NPs and compared our characterization data to those supplied by manufacturers. Discrepancies were noted and then measured values were used to assess the biological impact of these materials on three mammalian cell lines—A549, HepG2 and J774A.1 cells. Cell toxicity assays showed that all Mn2O3 particles exhibited cytotoxic effects that may be correlated, at least in part, to the production of reactive oxygen species. All eight nanoforms also activated caspase 3 but not caspase 1, although the magnitude of these changes varied greatly between materials.

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Section: Resultscontrasting

confidence: 99%

Section: Resultssupporting

confidence: 92%

Characterization and Cellular Toxicity Studies of Commercial Manganese Oxide Nanoparticles

Johnston,

Du,

Zborowski

et al. 2024

Nanomaterials

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“…Interestingly, the high surface reactivity of our NiO NPs (23.8 nM), as measured by ROS, would seem to suggest that the nanoparticles are quite reactive with possibly increased dissolution that would in turn result in a greater likelihood for toxicity. However, similar to the Kunc et al study, which reported that ROS did not appear to impact cytotoxicity [ 44 ], ROS did not appear to impact the acute toxicity results in our studies. Thus, as mentioned above, it appears that a factor that could be influential in determining acute toxicity is agglomeration/aggregation, which may have been responsible for the similar MMAD values of the MPs and NPs in the inhalation studies.…”

Section: Discussionsupporting

confidence: 89%

An Assessment of the Oral and Inhalation Acute Toxicity of Nickel Oxide Nanoparticles in Rats

Lyons-Darden¹,

Blum

Schooley

et al. 2023

Nanomaterials

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Nickel oxide nanoparticles (NiO NPs) have been the focus of many toxicity studies. However, acute toxicity studies that identify toxicological dose descriptors, such as an LC50 or LD50, are lacking. In this paper, the acute toxicity of NiO NPs was evaluated in albino-derived Sprague-Dawley rats through OECD guideline studies conducted by both the oral and inhalation routes of exposure. The animals were assessed for mortality, body weight, behavioral observations, and gross necropsy. Results from previously conducted (unpublished) acute inhalation studies with larger NiO microparticles (MPs) are also included for comparison. Mortality, the primary endpoint in acute toxicity studies, was not observed for rats exposed to NiO NPs via either the oral or inhalation exposure routes, with a determined LD50 of >5000 mg/kg and an LC50 >5.42 mg/L, respectively. Our results suggest that these NiO NPs do not exhibit serious acute toxicity in rats or warrant an acute toxicity classification under the current GHS classification criteria. This aligns with similar results for NiO MPs from this and previously published studies.

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“…The BMC concentration was also expressed in terms of the SSA of each particle, which takes into account the available surface atoms for interaction, and which is proposed as a more appropriate dose metric for MONPs with negligible solubility compared to mass dose [ 64 ]. For the NPs used in this study, experimentally derived SSA values were available from recent publications [ 65 , 66 ]. However, for MPs, only the manufacturer-provided ranges were available (See Section 4 for details).…”

Section: Discussionmentioning

confidence: 99%

Toxicity of Metal Oxide Nanoparticles: Looking through the Lens of Toxicogenomics

Boyadzhiev,

Wu,

Avramescu

et al. 2023

IJMS

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The impact of solubility on the toxicity of metal oxide nanoparticles (MONPs) requires further exploration to ascertain the impact of the dissolved and particulate species on response. In this study, FE1 mouse lung epithelial cells were exposed for 2–48 h to 4 MONPs of varying solubility: zinc oxide, nickel oxide, aluminum oxide, and titanium dioxide, in addition to microparticle analogues and metal chloride equivalents. Previously published data from FE1 cells exposed for 2–48 h to copper oxide and copper chloride were examined in the context of exposures in the present study. Viability was assessed using Trypan Blue staining and transcriptomic responses via microarray analysis. Results indicate material solubility is not the sole property governing MONP toxicity. Transcriptional signaling through the ‘HIF-1α Signaling’ pathway describes the response to hypoxia, which also includes genes associated with processes such as oxidative stress and unfolded protein responses and represents a conserved response across all MONPs tested. The number of differentially expressed genes (DEGs) in this pathway correlated with apical toxicity, and a panel of the top ten ranked DEGs was constructed (Hmox1, Hspa1a, Hspa1b, Mmp10, Adm, Serpine1, Slc2a1, Egln1, Rasd1, Hk2), highlighting mechanistic differences among tested MONPs. The HIF-1α pathway is proposed as a biomarker of MONP exposure and toxicity that can help prioritize MONPs for further evaluation and guide specific testing strategies.

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Physical Characterization and Cellular Toxicity Studies of Commercial NiO Nanoparticles

Cited by 8 publications

References 34 publications

Characterization and Cellular Toxicity Studies of Commercial Manganese Oxide Nanoparticles

Characterization and Cellular Toxicity Studies of Commercial Manganese Oxide Nanoparticles

An Assessment of the Oral and Inhalation Acute Toxicity of Nickel Oxide Nanoparticles in Rats

Toxicity of Metal Oxide Nanoparticles: Looking through the Lens of Toxicogenomics

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