“…Due to its ease of operation, cost, standardization, reproducibility, and sensitivity, this bioassay has often been used to estimate the toxicity of sediment extracts [2]. The Microtox assay has also been used to measure the toxicity of pure substances [3][4][5][6], effluents [7][8][9], aqueous and organic extracts [10,11], and in solid phase [12,13].…”