Physical-Chemical Properties of the Support Immobead 150 Before and After the Immobilization Process of Lipase

Matte, Carla Roberta; Bordinhão, Carolina; Poppe, Jakeline Kathiele; Benvenutti, Edílson V.; Costa, Tânia Maria Haas; Rodrigues, Rafael C.; Hertz, Plinho Francisco; Ayub, Marco Antônio Záchia

doi:10.21577/0103-5053.20160319

Cited by 11 publications

(12 citation statements)

References 31 publications

(48 reference statements)

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“…As time passed, it became tougher to occupy the available vacant surface sites due to the repelling forces amongst the solute molecules that occurred in the solid and bulk phases [32]. This was implicated by the reduction of the pore size of the carrier by way of increasing the original protein loading, which significantly constrained the diffusion of enzyme molecules to its interior side [33]. Therefore, it was anticipated that these aggregates would be multiplied at the high enzyme concentration, which would lead to a lower rate of diffusion to the carrier microenvironment [15].…”

Section: Adsorption Kinetics Studiesmentioning

confidence: 99%

Adsorption Kinetics of Cellulase and Xylanase Immobilized on Magnetic Mesoporous Silica

et al. 2019

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The adsorption kinetics of cellulase and xylanase immobilized on magnetically separable, hierarchically ordered mesocellular mesoporous silica (M-HMMS) was investigated. The adsorption of cellulase on M-HMMS followed pseudo-secondorder kinetics while the xylanase adsorption followed the Avrami model. Intraparticle and film diffusion also affected this process. These results enable in-depth knowledge of the cellulase and xylanase adsorption mechanisms on M-HMMS for better future applications by improving the stability of the immobilization.

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Section: Adsorption Kinetics Studiesmentioning

confidence: 99%

Adsorption Kinetics of Cellulase and Xylanase Immobilized on Magnetic Mesoporous Silica

et al. 2019

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“…Nitrogen physisorption studies have revealed that, the pore volume of thiol modified CO-MTS materials decreases from 1.0336 cm 3 /g before immobilization to 0.8169 cm 3 /g after trypsin immobilization [32]. The decrease in pore volume after enzyme immobilization is not strange and is due to coating of the support surface by the enzyme molecule [40].…”

Section: Immobilization Of Trypsin Enzymes On Co-mts Via Thiol-disulfmentioning

confidence: 99%

Mesoporous Materials Prepared Using Cashew Nut Shell Liquid and Castor Oil as Surfactants

Mgaya¹,

Mubofu²

2019

Mesoporous Materials - Properties and Applications

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Preparation of useful materials using renewable resources, which are not in competition with food production is of particular importance in the current efforts to replace non-renewable resources. One example of a potential renewable resource, which is attracting the attention of researchers in the preparation of useful materials is cashew nut shell liquid (CNSL). CNSL which is a by-product of cashew processing factories, is a mixture of four potential compounds, namely anacardic acid, cardanol, cardol and 2-methyl cardol. Among other potential applications, cashew nut shell liquid is a good template source for preparation of mesoporous materials. Heterogeneous catalysts prepared using CNSL templates are more efficient than those prepared using the commercially available templates. The pore sizes of mesoporous materials prepared using CNSL templates are large (up to 25 nm) enough to immobilize enzymes. Another renewable resource; castor oil, has also been reported to be a good template source for preparation of mesoporous materials. This chapter therefore is aimed at describing in detail the preparation, characterization and applications of mesoporous materials templated by cashew nut shell liquid and castor oil.

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“…e results obtained by HPTLC showed that CAL-Bo showed no catalytic activity in any of the combinations of organic solvents, possibly due to the immobilisation support to which the enzyme was attached. Lipase Candida CAL-Bo is immobilised in support Immobead 150, which is formed of methacrylate copolymers with epoxy functions, with an average particle size of 150-300 μm and presents a wide pore size range, with pores in the micropore and mesopore region [30]. In contrast, CAL-Bn is immobilised on a macroporous acrylic poly resin (methyl methacrylate-co-divinylbenzene), having an average particle size of 315-1000 μm and a pore diameter of ∼150Å [31,32].…”

Section: Enzymatic Transesterification Reaction Undermentioning

confidence: 99%

“…In contrast, CAL-Bn is immobilised on a macroporous acrylic poly resin (methyl methacrylate-co-divinylbenzene), having an average particle size of 315-1000 μm and a pore diameter of ∼150Å [31,32]. Both supports show hydrophobic properties; however, the pore size of the enzyme CAL-Bo (12-20Å) is smaller than that of CAL-Bn (∼150Å) and the pore size distribution is not homogeneous [30]. is characteristic is determinant for the enzyme-substrate coupling; a small pore size or inhomogeneous pore size allows only the smallest substrates to penetrate.…”

Section: Enzymatic Transesterification Reaction Undermentioning

confidence: 99%

Cochineal Waxy Residues as Source of Policosanol: Chemical Hydrolysis and Enzymatic Transesterification

Ramos-Zambrano

Herrera-Serrano

García-Dávila

et al. 2019

Journal of Chemistry

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The aim of this study was to obtain and characterise the long-chain alcohols present in policosanol derived from waste from the production of carminic acid, a natural colouring agent widely used in the food industry. The effectiveness of different methods designed for extraction of policosanol from waxy waste was investigated and its content and composition was determined. Triacontanol was the main component in policosanol produced by chemical processes, and it yields up to 13% by alkaline hydrolysis in water and chloroform extraction. Regarding enzymatic transesterification, policosanol was obtained using lipase Candida antarctica recombinant in Aspergillus niger (CAL-Bn) in a reaction medium with toluene. To improve the reaction, different acyl receptors, propanol, butanol, and isopropanol, were tested and molecular sieves were employed to maintain an anhydrous reaction medium. In this case, the policosanol was made up of other long-chain alcohols, but triacontanol was obtained in yields of up to 19% using isopropanol as an acyl receptor. Triacontanol has a great commercial value due to its effect as a promoter of plant growth, and these results contribute to the use and application of this agroindustrial waste in obtaining value-added products.

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Physical-Chemical Properties of the Support Immobead 150 Before and After the Immobilization Process of Lipase

Cited by 11 publications

References 31 publications

Adsorption Kinetics of Cellulase and Xylanase Immobilized on Magnetic Mesoporous Silica

Adsorption Kinetics of Cellulase and Xylanase Immobilized on Magnetic Mesoporous Silica

Mesoporous Materials Prepared Using Cashew Nut Shell Liquid and Castor Oil as Surfactants

Cochineal Waxy Residues as Source of Policosanol: Chemical Hydrolysis and Enzymatic Transesterification

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