Physical immobilization of laccase on an electrode by means of poly(ethyleneimine) microcapsules

Rochefort, Dominic; Kouisni, Lamfeddal; Gendron, Karine

doi:10.1016/j.jelechem.2008.01.027

Cited by 45 publications

(32 citation statements)

References 43 publications

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“…The microcapsules were rinsed with 50 mL of cyclohexane followed by 300 mL of water, recuperated by filtration and washed with three additional volumes of water. For the adsorption procedure, the microcapsules synthesized were suspended in 95% EtOH solution at 5 • C before deposition on the electrode surface (17,23).…”

Section: Apparatus and Methodsmentioning

confidence: 99%

Investigation of Microencapsulated Laccase as Enzyme Immobilization Template for Application in Bio-fuel Cell

Hébert

Rochefort

2008

ECS Trans.

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The electrochemical behavior of microencapsulated laccase with a non-conductive polymer (poly(ethyleneimine), PEI) adsorbed on a glassy carbon electrode (GC) had been investigated. A K m value of 76 µM and a sensitivity of 16.6 ± 0.1 nA/µM with a response time of 7.5 s had been determined for a laccase microencapsulated with PEI that was deposited on a bare GC electrode in a McIlvaine buffer at pH = 5.75 with p-phenylenediamine (PPD). The bioelectrode has found to have a permeability, P m , of 4.9 E-4 cm s -1 for PPD and too strong interactions with 2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonate) acid to observe any diffusion processes. The electrochemical and enzymatic oxidation of PPD was studied under various conditions to evaluate its reversibility and stability as a suitable redox mediator. RDE experiments showed a passivation for a glassy carbon electrode modified with empty poly(ethyleneimine) microcapsules under static conditions. Under hydrodynamic conditions however, the passivation was slowed down.

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Section: Apparatus and Methodsmentioning

confidence: 99%

Investigation of Microencapsulated Laccase as Enzyme Immobilization Template for Application in Bio-fuel Cell

Hébert

Rochefort

2008

ECS Trans.

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“…The stabilizing effect of these polymers having Fe(CN) 6 3À counterions on bilirubin oxidase immobilized on a membrane covered electrode was found [60]. In a more study Mo(CN) 8 4À anions were used as mediators for laccase entrapped in microcapsules deposited on the electrode surface [137]. It is also important to say that buffer solution of Fe(CN) 6 3À and bilirubin oxidase were used in cathodic compartment of membrane glucose-dioxygen biofuel cell developed for Sony Corp. [211,212].…”

Section: Biocathodes Employing Mediated Electrocatalysismentioning

confidence: 99%

“…Laccase was incorporated into crosslinked poly(ethylene imine) microcapsules, and this material was adsorbed on rough GC [137].…”

Section: Immobilization Of the Enzyme On The Biocathodementioning

confidence: 99%

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Biocathodes for Dioxygen Reduction in Biofuel Cells

Bilewicz

Opałło

2010

Fuel Cell Science

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“…Furthermore, the ABTS interaction with the PEI capsule core is a crucial drawback when applications involving shuttling of ABTS between the enzyme's active center and an electrode are considered (e.g., biofuel cells). 21 However, in this study, the diffusion of ABTS back to bulk solution is not required for activity measurement since the assay is based on oxygen consumption, and as a neutral molecule oxygen is not expected to interact with the capsule wall. The same applies to applications where diffusion of ABTS is not required yet induces either the color change of the substrate or the oxygen consumption due to the enzymatic reaction.…”

Section: Articlementioning

confidence: 99%

Printing of Polymer Microcapsules for Enzyme Immobilization on Paper Substrate

et al. 2011

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Poly(ethyleneimine) (PEI) microcapsules containing laccase from Trametes hirsuta (ThL) and Trametes versicolor (TvL) were printed onto paper substrate by three different methods: screen printing, rod coating, and flexo printing. Microcapsules were fabricated via interfacial polycondensation of PEI with the cross-linker sebacoyl chloride, incorporated into an ink, and printed or coated on the paper substrate. The same ink components were used for three printing methods, and it was found that laccase microcapsules were compatible with the ink. Enzymatic activity of microencapsulated TvL was maintained constant in polymer-based ink for at least eight weeks. Thick layers with high enzymatic activity were obtained when laccase-containing microcapsules were screen printed on paper substrate. Flexo printed bioactive paper showed very low activity, since by using this printing method the paper surface was not fully covered by enzyme microcapsules. Finally, screen printing provided a bioactive paper with high water-resistance and the highest enzyme lifetime.

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Physical immobilization of laccase on an electrode by means of poly(ethyleneimine) microcapsules

Cited by 45 publications

References 43 publications

Investigation of Microencapsulated Laccase as Enzyme Immobilization Template for Application in Bio-fuel Cell

Investigation of Microencapsulated Laccase as Enzyme Immobilization Template for Application in Bio-fuel Cell

Biocathodes for Dioxygen Reduction in Biofuel Cells

Printing of Polymer Microcapsules for Enzyme Immobilization on Paper Substrate

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