1985
DOI: 10.1128/jvi.53.2.495-500.1985
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Physical locus of the DNA polymerase gene and genetic maps of bacteriophage T5 mutants

Abstract: Segments of DNA that contained the DNA polymerase gene of bacteriophage T5 were isolated. The physical locus of the gene was identified by transforming Escherichia coli with purified DNA fragments generated by restriction enzyme digestions, and the transformed cells were used to rescue amber mutants of T5 with mutations in the gene for DNA polymerase. The method is applicable to any other gene that has mutations with low reversion frequencies. We studied the following mutations of the T5 DNA polymerase gene, r… Show more

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Cited by 6 publications
(6 citation statements)
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“…The relative distance traveled by the polypeptides coded by amber mutants T5am6 and T5D9 was somewhat contrary to what was expected from genetic recombination experiments (10). The values for the percentage of recombination for the T5am6 and T5D9 mutants in relation to the other mutants corresponded to D9 being at the extreme right end.…”
Section: Resultscontrasting
confidence: 71%
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“…The relative distance traveled by the polypeptides coded by amber mutants T5am6 and T5D9 was somewhat contrary to what was expected from genetic recombination experiments (10). The values for the percentage of recombination for the T5am6 and T5D9 mutants in relation to the other mutants corresponded to D9 being at the extreme right end.…”
Section: Resultscontrasting
confidence: 71%
“…Our intragenic map shows that the ts53 locus is very close to D9 and am6 (10). The temperature-sensitive polymerase isolated from T5ts53-infected cells was shown to be defective in the polymerization function without much effect on 3'->5' exonuclease activity (7).…”
Section: Resultsmentioning
confidence: 82%
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