Physical Map and Organization of
            <i>Arabidopsis thaliana</i>
            Chromosome 4

Schmidt, Renate; West, Joanne; Love, Karina; Lenehan, Zoe; Lister, Clare; Thompson, H L; Bouchez, David; Dean, Caroline

doi:10.1126/science.270.5235.480

Cited by 203 publications

(131 citation statements)

References 43 publications

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“…The Arabidopsis thaliana Columbia ecotype possesses 5S rDNA loci in the pericentromeric region of chromosomes 3, 4, and 5 (Murata et al 1997;Camilleri et al 1998;Fransz et al 1998;Schmidt et al 1995;Tutois et al 1999 and Fig. 1) for a total of ∼1000 5S rDNA genes.…”

Section: Resultsmentioning

confidence: 99%

“…The genome of A. thaliana has been sequenced (Arabidopsis Genome Initiative, 2000a), and physical maps using bacterial artificial chromosomes (BACs), transformation-competent artificial chromosomes (TACs), phage P1, and cosmids realized for each chromosome. 5S rDNA loci have been mapped using BACs on chromosome 3 (AGI, 2000b), YACs on chromosome 4 (Schmidt et al, 1995), YACs on the chromosome 5 left arm (Tutois et al 1999), and BACs on the chromosome 5 right arm (Kotani et al 1999;AGI, 2000c).…”

Section: Resultsmentioning

confidence: 99%

“…CIC YACs were mapped specifically for each 5S locus. YACs 9A5, 7E7, 7G3, and 9D3 were used for chromosome 3 loci 1, 2, and 3 and chromosome 4, respectively (this paper and Schmidt et al 1995). The major 5S locus of the left arm of chromosome 5 is contained in YACs 6A1-4E4 (Tutois et al 1999;Kotani et al 1999).…”

Section: Pcr-based Isolation Of Transcribed 5s Rdna Unitsmentioning

confidence: 99%

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Analysis of the 5S RNA Pool in Arabidopsis thaliana: RNAs Are Heterogeneous and Only Two of the Genomic 5S Loci Produce Mature 5S RNA

Cloix¹,

Tutois²,

Yukawa³

et al. 2001

Genome Res.

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One major 5S RNA, 120 bases long, was revealed by an analysis of mature 5S RNA from tissues, developmental stages, and polysomes in Arabidopsis thaliana. Minor 5S RNA were also found, varying from the major one by one or two base substitutions; 5S rDNA units from each 5S array of the Arabidopsis genome were isolated by PCR using CIC yeast artificial chromosomes (YACs) mapped on the different loci. By using a comparison of the 5S DNA and RNA sequences, we could show that both major and minor 5S transcripts come from only two of the genomic 5S loci: chromosome 4 and chromosome 5 major block. Other 5S loci are either not transcribed or produce rapidly degraded 5S transcripts. Analysis of the 5Ј-and 3Ј-DNA flanking sequence has permitted the definition of specific signatures for each 5S rDNA array.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Pcr-based Isolation Of Transcribed 5s Rdna Unitsmentioning

confidence: 99%

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Analysis of the 5S RNA Pool in Arabidopsis thaliana: RNAs Are Heterogeneous and Only Two of the Genomic 5S Loci Produce Mature 5S RNA

Cloix¹,

Tutois²,

Yukawa³

et al. 2001

Genome Res.

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“…AtHXKI is located on chromosome 4 (Schmidt et al, 1995) and is flanked by mi232 and g8300. AtHXK2 is located on chromosome 2 (Zachgo et al, 1996) and is flanked by mil48 and mi238 ( Figure 3).…”

Section: Molecular Characterization Of the Arabidopsis Hxk-encoding Gmentioning

confidence: 99%

Hexokinase as a sugar sensor in higher plants.

et al. 1997

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“…Meiotic recombination events in Arabidopsis thaliana (1) and maize (2) are unequally distributed along chromosomes, resulting in silent or hot spots of recombination, as in mammalian cells or yeast (3). In mammalian cells and yeast, high recombination frequency is confined to transcriptionally active regions, and this also has been concluded for maize (4).…”

mentioning

confidence: 92%

Arabidopsis thaliana mutants altered in homologous recombination

Masson¹,

Paszkowski²

1997

Proc. Natl. Acad. Sci. U.S.A.

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Homologous recombination contributes both to the generation of allelic diversity and to the preservation of genetic information. In plants, a lack of suitable experimental material has prevented studies of the regulatory and enzymatic aspects of recombination in somatic and meiotic cells. We have isolated nine Arabidopsis thaliana mutants hypersensitive to x-ray irradiation (xrs) and examined their recombination properties. For the three xrs loci described here, single recessive mutations were found to confer simultaneous hypersensitivities to the DNA-damaging chemicals mitomycin C (MMC s ) and͞or methyl methanesulfonate (MMS s ) and alterations in homologous recombination. Mutant xrs9 (Xray s , MMS s ) is reduced in both somatic and meiotic recombination and resembles yeast mutants of the rad52 epistatic group. xrs11 (Xray s , MMC s ) is deficient in the x-ray-mediated stimulation of homologous recombination in somatic cells in a manner suggesting a specific signaling defect. xrs4 (Xray s , MMS s , MMC s ) has a significant deficiency in somatic recombination, but this is accompanied by meiotic hyper-recombination. A corresponding phenotype has not been reported in other systems and thus this indicates a novel, plant-specific regulatory circuit linking mitotic and meiotic recombination.Many similarities in DNA repair and recombination have been noted between plants, yeast, and higher eukaryotes. Meiotic recombination events in Arabidopsis thaliana (1) and maize (2) are unequally distributed along chromosomes, resulting in silent or hot spots of recombination, as in mammalian cells or yeast (3). In mammalian cells and yeast, high recombination frequency is confined to transcriptionally active regions, and this also has been concluded for maize (4). As in other organisms (5), the frequency of meiotic recombination in plants is influenced by environmental factors (6), sequence diversity in interspecific crosses (7), and the direction of crossing (8). In somatic cells, rates of homologous recombination can be stimulated by DNA-damaging treatments (9-11). This also has been observed in bacteria, yeast, and mammalian cells (12). However, a closer comparison reveals that plant cells are highly resistant to DNA-damaging treatments and are significantly more prone to the induction of increased recombination levels (9-11) than animal cells (13). This feature may be relevant for the late differentiation of germ cells in plants and the potential transmission of somatic recombination events to the germ-line (14, 15). Interestingly, genomic changes are influenced by environmental stresses (16,17), and specific induction of intrachromosomal homologous recombination by heat and salt stress has been documented (10, 11). Thus, regulation of genome stability during plant development is especially important. To help decipher enzymatic and regulatory aspects of DNA metabolism in plants, we isolated A. thaliana mutants hypersensitive to x-ray irradiation (xrs for x-ray sensitive), which also showed elevated sensitivity to DN...

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Physical Map and Organization of Arabidopsis thaliana Chromosome 4

Cited by 203 publications

References 43 publications

Analysis of the 5S RNA Pool in Arabidopsis thaliana: RNAs Are Heterogeneous and Only Two of the Genomic 5S Loci Produce Mature 5S RNA

Analysis of the 5S RNA Pool in Arabidopsis thaliana: RNAs Are Heterogeneous and Only Two of the Genomic 5S Loci Produce Mature 5S RNA

Hexokinase as a sugar sensor in higher plants.

Arabidopsis thaliana mutants altered in homologous recombination

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