Physical mapping and molecular-cytogenetic analysis of substitutions and translocations involving chromosome 1D in synthetic hexaploid triticale

Hohmann, U.; Zoller, Jutta; Herrmann, Reinhold G.; Kazman, M. E.

doi:10.1007/s001220051116

Cited by 7 publications

(6 citation statements)

References 18 publications

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“…This finding is corroboration with Yu et al (2001), TW 5 lines are derived from the cross TL 2908 x HS 396 and the lines TW-5-41, TW-5-6 and TW-5-31 were carrying the 4,4 and 5 pairs of rye chromosome respectively and phenotypically they looked like triticale. Similar results were observed by Hohmann, et al (1999), in wheat and rye cross that one 1D addition, six 1D (1R) substitutions and nine 1D (1B) substitutions line in the study undertaken by them. The rye chromosomes are predominantly recognized by their large heterochromatic regions of the telomeres.…”

Section: Tw 4-tw6 Linessupporting

confidence: 87%

Identification of Alien Chromosome/Chromatin Introgressions in Triticale × Wheat Derived Stable Lines Through Molecular Cytogenetic Analysis

Jeberson,

Chaudhary,

Chahota

et al. 2021

J. Current Opinion Crop Sci.,

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Molecular cytogenetic study was conducted for identifying rye chromosome/chromatin translocation, substitution and addition in 43 triticale x wheat derived stable lines (BC1F7 and F7 generations) six different crosses using FISH (Fluorescence in situ hybridization) and GISH (Genomic in situ hybridization) analyses with different probes. Rye chromosomes/chromatin was identified in 17 BC1F7 and 12 F7 lines by these two analyses. The GISH and FISH techniques worked satisfactorily for identifying the presence of rye chromatin at metaphase. All the lines were associated with alien chromosome translocation, addition or substitution except a few lines. Most of the recombinants present in TW 1, TW 2 and TW 6 crosses were observed to be associated with important alien chromatin translocation like 1BL/1RS, substitutions 1R (1D), 5R (5D) and combination of both i.e., 1BL/1RS + 5R (5D) and in some cases presence of more than 4 rye chromosomes. The crosses TW 3 and TW 5 possessed 8 to 14 rye chromosomes in their chromosome constitution. So, their morphological expression was similar to triticale. These reconstituted lines will be of immense application for the future wheat breeding programmes.

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Section: Tw 4-tw6 Linessupporting

confidence: 87%

Identification of Alien Chromosome/Chromatin Introgressions in Triticale × Wheat Derived Stable Lines Through Molecular Cytogenetic Analysis

Jeberson,

Chaudhary,

Chahota

et al. 2021

J. Current Opinion Crop Sci.,

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“…PCR amplification with microsatellites mapped in all wheat D genome chromosomes, but 1D and 2D, may be associated with the presence of wheat-rye (Lukaszewski & Gustafson, 1983) or wheat-wheat translocation (Hohmann et al, 1999;Leonova et al 2005). However, studies have shown that alterations on the expression of certain genes in triticale compared to the parental wheat lines would result from the effect of rye chromosomes due to the change of triticale genomic composition, rather than being associated with the presence of wheat-rye translocation (Leonova et al, 2005).…”

Section: Resultsmentioning

confidence: 99%

Genetic diversity of Brazilian triticales evaluated with genomic wheat microsatellites

Costa

Albuquerque

Nascimento

et al. 2007

Pesq. agropec. bras.

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-The objective of this work was to determine the genetic variability available for triticale (X Triticosecale Wittmack) crop improvement in Brazil. Forty-two wheat genomic microsatellites were used to estimate the molecular diversity of 54 genotypes, which constitute the base of one of the major triticale breeding programs in the country. Average heterozygosity was 0.06 and average and effective number of alleles per locus were 2.13 and 1.61, respectively, with average allelic frequency of 0.34. The set of genomic wheat microsatellites used clustered the genotypes into seven groups, even when the germplasm was originated primarily from only two triticale breeding programs, a fact reflected on the average polymorphic information content value estimated for the germplasm (0.36). The 71.42% transferability achieved for the tested microsatellites indicates the possibility of exploiting these transferable markers in further triticale genetic and breeding studies, even those mapped on the D genome of wheat, when analyzing hexaploid triticales.Index terms: X Triticosecale, polymorphism information content, transferability, heterozygosity, number of alleles, frequency of alleles. Diversidade genética de triticales brasileiros avaliada com microssatélites genômicos de trigoResumo -O objetivo deste trabalho foi determinar a variabilidade disponível para o melhoramento de triticale (X Triticosecale Wittmack) no Brasil. Quarenta e dois microssatélites de trigo foram empregados para estimar a diversidade molecular de 54 genótipos, que constituem a base de um dos principais programas de melhoramento da espécie no país. A heterozigosidade média foi 0,06, e os números médio e efetivo de alelos por lócus foram de 2,13 e 1,61, respectivamente, com freqüência alélica média de 0,34. O conjunto de microssatélites de trigo possibilitou reunir os genótipos em sete grupos, mesmo que o germoplasma utilizado seja originado de apenas duas instituições de pesquisa, o que refletiu em baixo índice de polimorfismo médio (0,36). A taxa de transferência dos marcadores testados (71,42%) indica a possibilidade de uso desses microssatélites de trigo, até mesmo os mapeados no genoma D da espécie, na análise de triticales hexaplóides em futuros trabalhos de genética e melhoramento de triticale.Termos para indexação: X Triticosecale, índice de polimorfismo, transferabilidade, heterozigosidade, número efetivo de alelos, freqüência alélica.

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“…Hohmann et al [28] have revealed the existence of a wide spectrum of 1A/1D and 1B/1D recombinations and translocations upon the development of substituted hexaploid tirticale lines containing 1D chromosome. Amplification of the fragments specific to microsatellite markers of D genome was observed by Tams et al [18], who showed that three out of thirty-nine D genome markers produced PCR products in hexaploid triticale species.…”

Section: Discussionmentioning

confidence: 98%

Molecular Analysis of the Triticale Lines with Different Vrn Gene Systems Using Microsatellite Markers and Hybridization In Situ

Леонова

et al. 2005

Russ J Genet

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Hexaploid triticale ( × Triticosecale Wittmack) lines were examined using molecular markers and the hybridization in situ technique. Triticale lines were generated based on wheat varieties differing by the Vrn gene systems and the earing times. Molecular analysis was performed using Xgwm and Xrms microsatellite markers with the known chromosomal localization in the common wheat Triticum aestivum , and rye Secale cereale genomes. Comparative molecular analysis of triticale lines and their parental forms showed that all lines contained A and B genomes of common wheat and also rye homoeologous chromosomes. In the three lines the presence of D genome markers, mapped to the chromosomes 2D and 7D, was demonstrated. This was probably the consequence of the translocations of homoeologous chromosomes from wheat genomes, which took part during the process of triticale formation. The data obtained by use of genomic in situ hybridization supported the data of molecular genetic analysis. In none of the lines wheat-rye translocations or recombinations were observed. These findings suggest that the change of the period between the seedling appearance and earing time in triticale lines compared to the initial wheat lines, resulted from the inhibitory effect of rye genome on wheat vernalization genes. PLANT GENETICS

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Physical mapping and molecular-cytogenetic analysis of substitutions and translocations involving chromosome 1D in synthetic hexaploid triticale

Cited by 7 publications

References 18 publications

Identification of Alien Chromosome/Chromatin Introgressions in Triticale × Wheat Derived Stable Lines Through Molecular Cytogenetic Analysis

Identification of Alien Chromosome/Chromatin Introgressions in Triticale × Wheat Derived Stable Lines Through Molecular Cytogenetic Analysis

Genetic diversity of Brazilian triticales evaluated with genomic wheat microsatellites

Molecular Analysis of the Triticale Lines with Different Vrn Gene Systems Using Microsatellite Markers and Hybridization In Situ

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