Physical mapping of 14 new DNA markers isolated from the human distal Xp region

Wapenaar, Martin C.; Petit, Christine; Basler, Elizabeth; Ballabio, Andrea; Henke, Anja; Rappold, Gudrun; Paassen, H.M.B. van; Blonden, Lau; Ommen, G.J.B. van

doi:10.1016/0888-7543(92)90217-g

Cited by 10 publications

(1 citation statement)

References 45 publications

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“…On the basis of these findings, further mapping of the breakpoint region was achieved for seven patients (LWD1, LWD6, LWD7, LWD10, LWD15, LWD16, and ISS30) by use of SNP analysis. Cosmid P0117 contains the microsatellite marker P117 (DXYS417) (Wapenaar et al 1992), and further SNP markers were selected in the vicinity of P117 (three distal and three proximal markers [see fig. 3]).…”

Section: Resultsmentioning

confidence: 99%

Identification of a Major Recombination Hotspot in Patients with Short Stature and SHOX Deficiency

Schneider

Sabherwal

Jantz

et al. 2005

The American Journal of Human Genetics

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Human growth is influenced not only by environmental and internal factors but also by a large number of different genes. One of these genes, SHOX, is believed to play a major role in growth, since defects in this homeobox-containing gene on the sex chromosomes lead to syndromal short stature (Leri-Weill dyschondrosteosis, Langer mesomelic dysplasia, and Turner syndrome) as well as to idiopathic short stature. We have analyzed 118 unrelated patients with Leri-Weill dyschondrosteosis and >1,500 patients with idiopathic short stature for deletions encompassing SHOX. Deletions were detected in 34% of the patients with Leri-Weill dyschondrosteosis and in 2% of the patients with idiopathic short stature. For 27 patients with Leri-Weill dyschondrosteosis and for 6 with idiopathic short stature, detailed deletion mapping was performed. Analysis was performed by polymerase chain reaction with the use of pseudoautosomal polymorphic markers and by fluorescence in situ hybridization with the use of cosmid clones. Here, we show that, although the identified deletions vary in size, the vast majority (73%) of patients tested share a distinct proximal deletion breakpoint. We propose that the sequence present within this proximal deletion breakpoint "hotspot" region predisposes to recurrent breaks.

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Section: Resultsmentioning

confidence: 99%